Conformation Variation and Tunable Protein Adsorption through Combination of Poly(acrylic acid) and Antifouling Poly(N-(2-hydroxyethyl) acrylamide) Diblock on a Particle Surface

Abstract

Adsorption and desorption of proteins on biomaterial surfaces play a critical role in numerous biomedical applications. Spherical diblock polymer brushes (polystyrene with photoiniferter (PSV) as the core) with different block sequence, poly(acrylic acid)-b-poly(N-(2-hydroxyethyl) acrylamide) (PSV@PAA-b-PHEAA) and poly(N-(2-hydroxyethyl) acrylamide)-b-poly(acrylic acid) (PSV@PHEAA-b-PAA) were prepared via surface-initiated photoiniferter-mediated polymerization (SI-PIMP) and confirmed by a series of characterizations including TEM, Fourier transform infrared (FTIR) and elemental analysis. Both diblock polymer brushes show typical pH-dependent properties measured by dynamic light scattering (DLS) and Zeta potential. It is interesting to find out that conformation of PSV@PAA-b-PHEAA uniquely change with pH values, which is due to cooperation of electrostatic repulsion and steric hindrance. High-resolution turbidimetric titration was applied to explore the behavior of bovine serum albumin (BSA) binding to diblock polymer brushes, and the protein adsorption could be tuned by the existence of PHEAA as well as apparent PAA density. These studies laid a theoretical foundation for design of diblock polymer brushes and a possible application in biomedical fields.

Sequence Effects on the Salt-Enhancement Behavior of Polyelectrolytes Adsorption

“…Polyelectrolytes (PEs) are charged polymers, which are ubiquitous in materials science, environmental science, bioscience, industrial processing, and so on. In particular, the PEs play an important role in biological systems because of the complicated structure formation, which results in functionalization, derived from the electrostatic interactions through specific ways such as adsorbing on an oppositely charged surface. − In fact, the adsorption properties of PEs on an oppositely charged surface are governed by not only electrostatic interactions but also a complex interplay from different origins such as ion–ion correlations and steric effect. Furthermore, those complicated interactions are regulated by plenty of factors such as salt concentration, pH, surface charge density, solvent quality, chain length, and monomer sequence, just to name a few. , Among these factors, the salt effect on the adsorption of PEs has been extensively investigated through theories, − simulations, − and experiments. − …”

Transparent and photochromic poly(hydroxyethyl acrylate–acrylamide)/WO3 hydrogel with antibacterial properties against bacterial keratitis in contact lens

“…However, p(HEA‐AAm)/WO 3 and p(HEA‐AAm)/b‐WO 3 hydrogels revealed spherical‐like particles that are uniformly dispersed with large particle size distribution bound within the polymer matrix (Figure 4). 58,59 The DLS was used to determine the particle sizes and distribution of the hydrogels (Figure S5(a–c)). The hydrogels displayed uniformity in particle distribution with polydispersity index <0.08.…”

Graphene composites with hydrogel

“…PHEAA and P (HEAA/AA) nanogels were prepared by the inverse microemulsion polymerization method [63]. Wang et al [64] synthesized different types of branched polymers, spherical diblock polymer brushes. Like core, they use polystyrene with photoinifer (PSV).…”