Conformal polymer coatings for pancreatic islets transplantation

Blasi, Paolo; Luca, Giovanni; Mancuso, Francesca; Schoubben, Aurelie Marie Madeleine; Calvitti, Mario; Giovagnoli, Stefano; Basta, Giuseppe; Becchetti, Ennio; Ricci, Maurizio; Calafiore, Riccardo

doi:10.1016/j.ijpharm.2012.10.010

Cited by 18 publications

(7 citation statements)

References 45 publications

(66 reference statements)

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“…Probiotics could benefit the health of the host when sufficient numbers of them colonize in the intestine (Blasi and others ). Therefore, it is important to protect the probiotics from damage by the acidic environment of the human stomach.…”

Section: Resultsmentioning

confidence: 99%

“…Studies by Heidebach and others. reported that sodium alginate was one of the most viable encapsulation materials, noted for its safety and low toxicity (Albertini and others ; Heidebach and others ; Blasi and others ). Sodium alginate is a natural polysaccharide derived from brown algae comprising of a linear chain of 1,4‐linked‐β‐D‐mannuronic acid (M) and α‐L‐glucuronic acid (G) residues.…”

Section: Introductionmentioning

confidence: 99%

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Preparation of Acid‐Resistant Microcapsules with Shell‐Matrix Structure to Enhance Stability of Streptococcus Thermophilus IFFI 6038

Zhou

Chen

et al. 2017

Journal of Food Science

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Microencapsulation is an effective technology used to protect probiotics against harsh conditions. Extrusion is a commonly used microencapsulation method utilized to prepare probiotics microcapsules that is regarded as economical and simple to operate. This research aims to prepare acid-resistant probiotic microcapsules with high viability after freeze-drying and optimized storage stability. Streptococcus thermophilus IFFI 6038 (IFFI 6038) cells were mixed with trehalose and alginate to fabricate microcapsules using extrusion. These capsules were subsequently coated with chitosan to obtain chitosan-trehalose-alginate microcapsules with shell-matrix structure. Chitosan-alginate microcapsules (without trehalose) were also prepared using the same method. The characteristics of the microcapsules were observed by measuring the freeze-dried viability, acid resistance, and long-term storage stability of the cells. The viable count of IFFI 6038 in the chitosan-trehalose-alginate microcapsules was 8.34 ± 0.30 log CFU g after freeze-drying (lyophilization), which was nearly 1 log units g greater than the chitosan-alginate microcapsules. The viability of IFFI 6038 in the chitosan-trehalose-alginate microcapsules was 6.45 ± 0.09 log CFU g after 120 min of treatment in simulated gastric juices, while the chitosan-alginate microcapsules only measured 4.82 ± 0.22 log CFU g . The results of the long-term storage stability assay indicated that the viability of IFFI 6038 in chitosan-trehalose-alginate microcapsules was higher than in chitosan-alginate microcapsules after storage at 25 °C. Trehalose played an important role in the stability of IFFI 6038 during storage. The novel shell-matrix chitosan-trehalose-alginate microcapsules showed optimal stability and acid resistance, demonstrating their potential as a delivery vehicle to transport probiotics.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Preparation of Acid‐Resistant Microcapsules with Shell‐Matrix Structure to Enhance Stability of Streptococcus Thermophilus IFFI 6038

Zhou

Chen

et al. 2017

Journal of Food Science

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“…In this regard, microencapsulation might be superior to any kind of macro device. Indeed, microencapsulated islets from various sources have generally shown appropriate competence for insulin release, but the kinetics are often compromised with delayed insulin response in vitro and in vivo [75,[85][86][87][88]. This might be related to the size of the capsules (layer-by-layer encoated nanocapsules show improvement in insulin kinetics [59]), the composition of encapsulation material, and possibly pericapsular fibrotic overgrowth.…”

Section: Kinetics Of Insulin Release and Transplantation Sitesmentioning

confidence: 99%

Transplantable bioartificial pancreas devices: current status and future prospects

Ludwig

2015

Langenbecks Arch Surg

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“…Is there any published evidence suggesting that this age is optimal? Most studies of pig NICC transplantation into mice have used donors of 1 to 7 days of age .…”

Section: Is There An Optimal Age For Isolation Of Iccs From Neonatal mentioning

confidence: 99%

Islet xenotransplantation: what is the optimal age of the islet‐source pig?

Nagaraju

Bottino

Wijkstrom

et al. 2014

Xenotransplantation

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Conformal polymer coatings for pancreatic islets transplantation

Cited by 18 publications

References 45 publications

Preparation of Acid‐Resistant Microcapsules with Shell‐Matrix Structure to Enhance Stability of Streptococcus Thermophilus IFFI 6038

Preparation of Acid‐Resistant Microcapsules with Shell‐Matrix Structure to Enhance Stability of Streptococcus Thermophilus IFFI 6038

Transplantable bioartificial pancreas devices: current status and future prospects

Islet xenotransplantation: what is the optimal age of the islet‐source pig?

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