Confocal scanning laser microscopy of morphometric human sperm parameters: correlation with acrosin profiles and fertilizing capacity

Sofikitis, Nikolaos; Miyagawa, Ikuo; Zavos, Panayiotis M.; Toda, Toshiko; Iino, Akihiro; Terakawa, Naoki

doi:10.1016/s0015-0282(16)56894-0

Cited by 44 publications

(39 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Reduced midpiece length could also negatively affect fertilization success or competitiveness, as it results in a decrease in the quantity or size of mitochondria contained in each spermatozoon [38], and hence in reduced power output and swimming velocity [35,39]. A positive correlation between midpiece length and fertilization success has indeed been found both in vitro [40] and in vivo [41]. Midpiece length and abnormalities in midpiece structure have been found to be highly heritable in both mammals and birds [38,42].…”

Section: (C) Sperm Qualitymentioning

confidence: 99%

The genetic rescue of two bottlenecked South Island robin populations using translocations of inbred donors

et al. 2013

View full text Add to dashboard Cite

Populations forced through bottlenecks typically lose genetic variation and exhibit inbreeding depression. 'Genetic rescue' techniques that introduce individuals from outbred populations can be highly effective in reversing the deleterious effects of inbreeding, but have limited application for the majority of endangered species, which survive only in a few bottlenecked populations. We tested the effectiveness of using highly inbred populations as donors to rescue two isolated and bottlenecked populations of the South Island robin (Petroica australis). Reciprocal translocations significantly increased heterozygosity and allelic diversity. Increased genetic diversity was accompanied by increased juvenile survival and recruitment, sperm quality, and immunocompetence of hybrid individuals (crosses between the two populations) compared with inbred control individuals (crosses within each population). Our results confirm that the implementation of 'genetic rescue' using bottlenecked populations as donors provides a way of preserving endangered species and restoring their viability when outbred donor populations no longer exist.

show abstract

Section: (C) Sperm Qualitymentioning

confidence: 99%

The genetic rescue of two bottlenecked South Island robin populations using translocations of inbred donors

et al. 2013

View full text Add to dashboard Cite

show abstract

“…When ultrasonographical control demonstrated that the tip of the needle had reached the hyperdense line of the rete testis negative pressure was applied and cells from the rete testis were aspirated. All aspirated cellular samples were observed via confocal scanning laser microscope‐computer assisted system (CSLM‐CAS; Sofikitis et al ., 1994a,b, 1996) and some of them via an epi‐fluorescent microscope [fluorescent in situ hybridization (FISH) procedures were also performed]. The main rete testis‐cellular populations of each man of group A or B after observation via CSLM‐CAS were processed for cryopreservation.…”

Section: Participantsmentioning

confidence: 99%

“…In addition, minor subfractions from each rete testis sample of the NOA men were processed for FISH procedures after the observation via CSLM‐CAS. The latter two microscopical modalities (CSLM‐CAS and FISH techniques) have been proven in our laboratory to be sufficient to differentiate among round germ cells (spermatogonia/primary spermatocytes, secondary spermatocytes and round spermatids) (Sofikitis et al ., 1994b, 1998a,b,c; Yamanaka et al ., 1997; Yamamoto et al ., 1999a, 2002a,b).…”

Section: Participantsmentioning

confidence: 99%

See 1 more Smart Citation

The role of ultrasonographically guided puncture of the human rete testis in the therapeutic management of nonobstructive azoospermia*

Kaponis¹,

Yiannakis²,

Tsoukanelis³

et al. 2003

Andrologia

View full text Add to dashboard Cite

We attempted to characterize the cells collected from the rete testis via ultrasonographically guided puncture. Unilateral puncture of the rete testis was performed in nine men with obstructive azoospermia and 51 men with nonobstructive azoospermia. All the aspirated samples from the rete testis were observed via confocal scanning laser microscope and some of them after fluorescent in situ hybridization techniques. Then therapeutic testicular biopsy was performed in the punctured testis of each man. Spermatozoa were found in all rete testis samples and all biopsy samples from obstructed men. Twenty-two nonobstructed men demonstrated absence of spermatozoa in biopsy samples. Twenty-nine nonobstructed men showed spermatozoa in biopsy material and 24 of these men (82%) had demonstrated spermatozoa in rete testis samples. There were no significant differences in fertilization and cleavage rate between intracytoplasmic sperm injection trials using biopsy spermatozoa and rete testis spermatozoa both in obstructed and nonobstructed men. Considering that puncture of the rete testis does not reduce the volume of testicular parenchyma, is less invasive and apparently causes less detrimental effect on testicular vasculature than biopsy, puncture of rete testis is recommended as first line approach for the treatment of azoospermic men. If puncture is negative for spermatozoa in nonobstructed men, biopsy is indicated.

show abstract

“…Details of ovarian stimulation and oocyte recovery in our facilities have been published previously (7). After treatment with 0.1% bovine testicular hyaluronidase (type VIII; Sigma Co., St. Louis, MO) for 2 rain, the oocytes were freed from cumulus or coronal cells and transferred into Human Tubal Fluid medium (Irvine Scientific, Santa Ana, CA) supplemented with 15% patients serum (modified HTF medium) and incubated at 37°C under 5% CO2 for 3 to 5 hr.…”

Section: Oocyte Preparationmentioning

confidence: 99%

Ooplasmic round spermatid nuclear injection procedures as an experimental treatment for nonobstructive azoospermia

Yamanaka

Sofikitis

Miyagawa

et al. 1997

J Assist Reprod Genet

Self Cite

View full text Add to dashboard Cite

Confocal scanning laser microscopy of morphometric human sperm parameters: correlation with acrosin profiles and fertilizing capacity

Cited by 44 publications

References 12 publications

The genetic rescue of two bottlenecked South Island robin populations using translocations of inbred donors

The genetic rescue of two bottlenecked South Island robin populations using translocations of inbred donors

The role of ultrasonographically guided puncture of the human rete testis in the therapeutic management of nonobstructive azoospermia*

Ooplasmic round spermatid nuclear injection procedures as an experimental treatment for nonobstructive azoospermia

Contact Info

Product

Resources

About