Confocal Raman spectroscopy: Evaluation of a non-invasive technique for the detection of topically applied ketorolac tromethamine in vitro and in vivo

Bertens, Christian J F; Zhang, Shuo; Erckens, Roel J.; Biggelaar, Frank J H M van den; Berendschot, Tos T. J. M.; Webers, Carroll A.B.; Nuijts, Rudy M.M.A.; Gijs, Marlies

doi:10.1016/j.ijpharm.2019.118641

Cited by 13 publications

(10 citation statements)

References 35 publications

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“…This peak intensity ratio approach already has been reported and validated for ex vivo Raman investigation in human aqueous humor [33]. In a previous study, we developed a tailored MATLAB (Version 2017b, The Mathworks Inc., Natick, MA, United States) data processing program for in vitro and in vivo Raman detection of ocular drug topically delivered to the animal eyes [34,35] and demonstrated that with proper data processing, the molecular information can be extracted non-invasively from biological fluids in-situ and ex vivo.…”

Section: Discussionmentioning

confidence: 99%

Raman spectroscopic detection of interleukin-10 and angiotensin converting enzyme

Zhang

Mee

Erckens

et al. 2021

J. Eur. Opt. Soc.-Rapid Publ.

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Section: Discussionmentioning

confidence: 99%

Raman spectroscopic detection of interleukin-10 and angiotensin converting enzyme

Zhang

Mee

Erckens

et al. 2021

J. Eur. Opt. Soc.-Rapid Publ.

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“…For instance, Raman spectroscopy, although still requiring optimization, holds promise to detect aqueous humor content. [66][67][68] With specific protocols this technique was also able to detect miRNAs in vitro. 69 In conclusion, small RNA sequencing revealed seven miRNAs with differential expression in AH of POAG patients compared to cataract controls, while no differences were observed in plasma.…”

Section: Differentially Expressed Mirnas As Diagnostic Biomarkersmentioning

confidence: 99%

Small RNA Sequencing of Aqueous Humor and Plasma in Patients With Primary Open-Angle Glaucoma

Hubens

Krauskopf

Beckers

et al. 2021

Invest. Ophthalmol. Vis. Sci.

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Identify differentially expressed microRNAs (miRNAs) in aqueous humor (AH) and blood of primary open-angle glaucoma (POAG) patients by using small RNA sequencing. These may provide insight into POAG pathophysiology or serve as diagnostic biomarker.METHODS. AH and plasma of nine POAG patients and 10 cataract control patients were small RNA sequenced on Illumina NovaSeq 6000. Identification of gene transcripts targeted by differentially expressed miRNAs was done with miRWalk and MirPath. These targets were used for pathway analysis and Gene Ontology enrichment. Diagnostic potential was evaluated by receiver operating characteristics analysis. RESULTS.We identified 715 miRNAs in plasma and 62 miRNAs in AH. Plasma miRNA profile did not differ between POAG and control. In contrast, in AH, seven miRNAs were differentially expressed. Hsa-miR-30a-3p, hsa-miR-143-3p, hsa-miR-211-5p, and hsa-miR-221-3p were upregulated, whereas hsa-miR-92a-3p, hsa-miR-451a, and hsa-miR-486-5p were downregulated in POAG. Compared to previous studies, hsa-mir-143-3p, hsa-miR-211-5p, and hsa-miR-221-3p were reported previously, strengthening their involvement in POAG whereas hsa-miR-30a-3p, hsa-miR-92a-3p, and hsa-miR-486-5p are implicated in POAG for the first time. Identified gene transcripts were involved in several pathways, some implicated in glaucoma before (e.g., TGF-β and neurotrophin signaling), whereas others are new (e.g., prolactin and apelin signaling). In respect to diagnostics, AH concentration of hsa-mir-143-3p had an area under the curve (AUC) of 0.889. Combined with hsa-miR-221-3p, AUC improved to 0.96. CONCLUSIONS.Small RNA sequencing identified seven differentially expressed miRNAs in AH of POAG patients. The differentially expressed miRNAs may be useful as POAG biomarkers or could become targets for new therapeutic strategies.

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“…All Raman spectra were processed by a self-developed MATLAB program (Version 2017b, The Mathworks Inc., Natick). 23…”

Section: Confocal Raman Spectroscopymentioning

confidence: 99%

Dark-field illumination in conjunction with confocal Raman spectroscopy for real-time noninvasive aqueous humor investigation

et al. 2020

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Confocal Raman spectroscopy: Evaluation of a non-invasive technique for the detection of topically applied ketorolac tromethamine in vitro and in vivo

Cited by 13 publications

References 35 publications

Raman spectroscopic detection of interleukin-10 and angiotensin converting enzyme

Raman spectroscopic detection of interleukin-10 and angiotensin converting enzyme

Small RNA Sequencing of Aqueous Humor and Plasma in Patients With Primary Open-Angle Glaucoma

Dark-field illumination in conjunction with confocal Raman spectroscopy for real-time noninvasive aqueous humor investigation

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