Confocal microscopy with a volume holographic filter

Barbastathis, George; Balberg, Michal; Brady, David J.

doi:10.1364/ol.24.000811

Cited by 98 publications

(34 citation statements)

References 8 publications

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“…Similarly, various point-of-care diagnostic devices have been developed and among them optical imaging and sensing techniques are highly advantageous as they can provide real-time, highresolution and highly sensitive quantitative information, potentially assisting rapid and accurate diagnosis. [30][31][32][33][34][35][36][37][38][39][40] To date, a number of optical techniques have been proposed for point-of-care diagnostics such as in vitro optical devices, [41][42][43][44][45][46][47][48][49][50][51][52][53] including portable optical imaging systems, optical microscopes integrated to cell phones or in vivo optical devices, [54][55][56][57][58][59][60][61][62][63] involving confocal microscopy, microendoscopy and optical coherence tomography techniques. Among these approaches, lens-free computational on-chip imaging 64 has been an emerging technique that can eliminate the need for bulky and costly optical components while also preserving (or even enhancing in certain cases) the image resolution, field of view and sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Handheld high-throughput plasmonic biosensor using computational on-chip imaging

et al. 2014

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We demonstrate a handheld on-chip biosensing technology that employs plasmonic microarrays coupled with a lens-free computational imaging system towards multiplexed and high-throughput screening of biomolecular interactions for point-of-care applications and resource-limited settings. This lightweight and field-portable biosensing device, weighing 60 g and 7.5 cm tall, utilizes a compact optoelectronic sensor array to record the diffraction patterns of plasmonic nanostructures under uniform illumination by a single-light emitting diode tuned to the plasmonic mode of the nanoapertures. Employing a sensitive plasmonic array design that is combined with lens-free computational imaging, we demonstrate label-free and quantitative detection of biomolecules with a protein layer thickness down to 3 nm. Integrating large-scale plasmonic microarrays, our on-chip imaging platform enables simultaneous detection of protein mono-and bilayers on the same platform over a wide range of biomolecule concentrations. In this handheld device, we also employ an iterative phase retrieval-based image reconstruction method, which offers the ability to digitally image a highly multiplexed array of sensors on the same plasmonic chip, making this approach especially suitable for high-throughput diagnostic applications in field settings.

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Section: Introductionmentioning

confidence: 99%

Handheld high-throughput plasmonic biosensor using computational on-chip imaging

et al. 2014

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“…In effect, the pinhole method performs the same operation as a classical confocal microscope, while the correlation method acts more like a matched filter [36] measuring the amount of backscattered light bearing the same signature as the excitation light. The correlation method has a lower computational cost, because we do not need to transform the proximal field and reconstruct the distal field.…”

Section: Comparison Of the Digital Confocal And The Correlation Methodsmentioning

confidence: 99%

Digital confocal microscopy through a multimode fiber

Loterie¹,

Farahi²,

Papadopoulos³

et al. 2015

Opt. Express

146

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Acquiring high-contrast optical images deep inside biological tissues is still a challenging problem. Confocal microscopy is an important tool for biomedical imaging since it improves image quality by rejecting background signals. However, it suffers from low sensitivity in deep tissues due to light scattering. Recently, multimode fibers have provided a new paradigm for minimally invasive endoscopic imaging by controlling light propagation through them. Here we introduce a combined imaging technique where confocal images are acquired through a multimode fiber. We achieve this by digitally engineering the excitation wavefront and then applying a virtual digital pinhole on the collected signal. In this way, we are able to acquire images through the fiber with significantly increased contrast. With a fiber of numerical aperture 0.22, we achieve a lateral resolution of 1.5µm, and an axial resolution of 12.7µm. The point-scanning rate is currently limited by our spatial light modulator (20Hz). wide-field endoscopic imaging by using a single multimode optical fiber," Phys.

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“…8 The excitation Fe 2ϩ →(Fe 2ϩ )* takes place at about 1200 nm. In addition, LiNbO 3 shows fundamental band-to-band absorption at short wavelengths, starting with a weak tail at about 600 nm, and becoming strong below 400 nm.…”

Section: Absorption Mechanisms In Linbo 3 :Fementioning

confidence: 99%

“…3 Inhomogeneous illumination excites electrons from high intensity areas. They migrate and are trapped in low intensity regions.…”

Section: Introductionmentioning

confidence: 99%

Temperature dependence of absorption in photorefractive iron-doped lithium niobate crystals

Panotopoulos

Luennemann

Buse

2002

Journal of Applied Physics

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We present experimental data showing a significant dependence of light absorption on temperature in photorefractive LiNbO 3 :Fe crystals. The results are successfully explained by assuming that the widths of the Fe 2ϩ absorption bands in the visible and in the infrared spectral region depend on temperature. The findings are of relevance for thermal fixing of holograms. Furthermore, a temperature-induced increase of the infrared absorption is promising for improved infrared recording.

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Confocal microscopy with a volume holographic filter

Cited by 98 publications

References 8 publications

Handheld high-throughput plasmonic biosensor using computational on-chip imaging

Handheld high-throughput plasmonic biosensor using computational on-chip imaging

Digital confocal microscopy through a multimode fiber

Temperature dependence of absorption in photorefractive iron-doped lithium niobate crystals

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