Confocal Microscopy Of The Eye.

Masters, Barry R.

doi:10.1117/12.962718

Cited by 3 publications

(2 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1). The paraxial method was used in the design of the objective (Masters 1990). The matrix method allows the determination of the front and back focal lengths of the system and the image location and magnification for different locations of the object.…”

Section: Retinal Objectivementioning

confidence: 99%

“…The increase in illumination of the object by increasing hole size comes at the expense of the contrast and optical sectioning capabilities of the microscope. With larger holes, the optical sections become thicker and more light is allowed through from the "out of focus" planes, thus reducing the contrast of the system (Masters 1990).…”

Section: Scanning Disc Pinhole Sizementioning

confidence: 99%

See 1 more Smart Citation

Observation of the retina using the tandem scanning confocal microscope

et al. 1996

View full text Add to dashboard Cite

Summary:A tandem scanning confocal microscope (TSCM) is currently being used to obtain high-resolution images of the human cornea in vivo. Advantages of confocal microscopy for in vivo imaging include optical sectioning and increased contrast through removal of scattered light. We have adapted the TSCM to view the retina in vivo by constructing an applanating lens and fitting the microscope with an imaging-intensifying camera of increased sensitivity. The microscope uses a spinning disc with 40,000 holes, each of 30 microns diameter, and a 100 W mercury arc lamp light source with a 455 nm long pass filter. The applanating lens is composed of three elements, two of which are movable for focusing. Images of a rabbit retina were obtained in vivo revealing the nerve fiber layer and blood vessels around the optic disc. The power density at the retina was calculated to be 3 mW/cm 2 , which is well below the power levels of a direct or indirect ophthalmoscope. Magnification of the retinal image was approximately 60× and a 1 mm wide area of retina was in view. This prototype TSCM system demonstrates that images of a retina in vivo are obtainable with confocal microscopy and that the sharpness is comparable to standard fundus camera photography. Further modifications to improve the light level and alterations in the design of the objective should improve the quality of the images obtained and achieve the enhanced resolution of which, in theory, the confocal microscope is capable.

show abstract

Section: Retinal Objectivementioning

confidence: 99%

Section: Scanning Disc Pinhole Sizementioning

confidence: 99%

Observation of the retina using the tandem scanning confocal microscope

et al. 1996

View full text Add to dashboard Cite

show abstract

Two- and three-dimensional visualization of the living cornea and ocular lens

Masters

1991

Machine Vis. Apps.

Self Cite

View full text Add to dashboard Cite

This paper describes the visualization of the living cornea and the in-situ ocular lens. Laser scanning confocal microscopy of a freshly enucleated rabbit eye was performed to obtain two-dimensional optical sections. These optical sections were used to reconstruct the threedimensional views of the cornea and ocular lens using the volume-rendering method. In the reflected light mode the cornea and the ocular lens are almost transparent and have extremely low contrast when observed in a normal light microscope. The images obtained with the confocal light microscope system shows submicron resolution in the image plane.The confocal light microscope provides high resolution, high contrast images of living ocular tissue. The image quality of the resulting confocal images rivals that obtained from electron microscope of fixed, stained, and coated tissue specimens. This paper demonstrates the quality of confocal microscope images and the feasibility of their three-dimensional reconstruction using computer volume-rendering techniques.

show abstract

Recombinant Protein-Stabilized Monodisperse Microbubbles with Tunable Size Using a Valve-Based Microfluidic Device

Angilè¹,

Vargo²,

Sehgal

et al. 2014

Langmuir

View full text Add to dashboard Cite

Microbubbles are used as contrast enhancing agents in ultrasound sonography and more recently have shown great potential as theranostic agents that enable both diagnostics and therapy. Conventional production methods lead to highly polydisperse microbubbles, which compromise the effectiveness of ultrasound imaging and therapy. Stabilizing microbubbles with surfactant molecules that can impart functionality and properties that are desirable for specific applications would enhance the utility of microbubbles. Here we generate monodisperse microbubbles with a large potential for functionalization by combining a microfluidic method and recombinant protein technology. Our microfluidic device uses an air-actuated membrane valve that enables production of monodisperse microbubbles with narrow size distribution. The size of microbubbles can be precisely tuned by dynamically changing the dimension of the channel using the valve. The microbubbles are stabilized by an amphiphilic protein, oleosin, which provides versatility in controlling the functionalization of microbubbles through recombinant biotechnology. We show that it is critical to control the composition of the stabilizing agents to enable formation of highly stable and monodisperse microbubbles that are echogenic under ultrasound insonation. Our protein-shelled microbubbles based on the combination of microfluidic generation and recombinant protein technology provide a promising platform for ultrasound-related applications.

show abstract

Confocal Microscopy Of The Eye.

Cited by 3 publications

References 58 publications

Observation of the retina using the tandem scanning confocal microscope

Observation of the retina using the tandem scanning confocal microscope

Two- and three-dimensional visualization of the living cornea and ocular lens

Recombinant Protein-Stabilized Monodisperse Microbubbles with Tunable Size Using a Valve-Based Microfluidic Device

Contact Info

Product

Resources

About