Confocal Microscopic Images of a Compost Particle

Chalmers, Jeffrey J.; Mauras, Cesar; Vir, Rahul

doi:10.1021/bp970107z

Cited by 6 publications

(2 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This principle was first used in cell physiology for morphological and biochemical studies of living cells (8,9). The potential of CLSM investigations has subsequently been applied in environmental sciences, e.g., for compost (10) and biofilm analyses (11), and in engineering, e.g., for the study of adsorption onto ionexchange resins (12).…”

Section: Introductionmentioning

confidence: 99%

Direct Measurement of pH Profiles in Immobilized Enzyme Carriers during Kinetically Controlled Synthesis Using CLSM

Spieß

Kasche

2001

Biotechnology Progress

View full text Add to dashboard Cite

Confocal laser scanning microscopy was applied to measure the pH value in the carrier of immobilized enzymes during the enzyme-catalyzed synthesis. pH profiles with a high resolution are shown, with the pH increasing in the core of the particles. Significant differences occur for different carrier material, particle size, porosity and surface modification. The increased pH value is identified as one of the reasons leading to reduced enzyme selectivity in the penicillin amidase catalyzed synthesis of cephalosporins and penicillins.

show abstract

Section: Introductionmentioning

confidence: 99%

Direct Measurement of pH Profiles in Immobilized Enzyme Carriers during Kinetically Controlled Synthesis Using CLSM

Spieß

Kasche

2001

Biotechnology Progress

View full text Add to dashboard Cite

show abstract

“…Confocal scanning laser microscopy was used to observe a partially degraded compost particle (Chalmers et al, 1997). When compared to typical epifluorescence microscopy, significant heterogeneity in the biofilm was observed.…”

Section: Industrial Wastesmentioning

confidence: 99%

Agricultural Wastes

Poggi‐Varaldo¹

1999

Water Environment Research

View full text Add to dashboard Cite

Use of confocal scanning laser microscopy to measure the concentrations of aerial and penetrative hyphae during growth of Rhizopus oligosporus on a solid surface

Nopharatana

Mitchell

Howes

2003

Biotech & Bioengineering

View full text Add to dashboard Cite

In order to develop a method for use in investigations of spatial biomass distribution in solid-state fermentation systems, confocal scanning laser microscopy was used to determine the concentrations of aerial and penetrative biomass against height and depth above and below the substrate surface, during growth of Rhizopus oligosporus on potato dextrose agar. Penetrative hyphae had penetrated to a depth of 0.445 cm by 64 h and showed rhizoid morphology, in which the maximum biomass concentration, of 4.45 mg dry wt cm(-3), occurred at a depth of 0.075 cm. For aerial biomass the maximum density of 39.54 mg dry wt (-3) occurred at the substrate surface. For both aerial and penetrative biomass, there were two distinct regions in which the biomass concentration decayed exponentially with distance from the surface. For aerial biomass, the first exponential decay region was up to 0.1 cm height. The second region above the height of 0.1 cm corresponded to that in which sporangiophores dominated. This work lays the foundation for deeper studies into what controls the growth of fungal hyphae above and below the surfaces of solid substrates.

show abstract

Confocal Microscopic Images of a Compost Particle

Cited by 6 publications

References 20 publications

Direct Measurement of pH Profiles in Immobilized Enzyme Carriers during Kinetically Controlled Synthesis Using CLSM

Direct Measurement of pH Profiles in Immobilized Enzyme Carriers during Kinetically Controlled Synthesis Using CLSM

Agricultural Wastes

Use of confocal scanning laser microscopy to measure the concentrations of aerial and penetrative hyphae during growth of Rhizopus oligosporus on a solid surface

Contact Info

Product

Resources

About