Confocal laser scanning microscopy of porcine skin: implications for human wound healing studies

Vardaxis, Nicholas J.; Brans, Toon; Boon, Mathilde E.; Kreis, R.W.; Marres, L. M.

doi:10.1046/j.1469-7580.1997.19040601.x

Cited by 147 publications

(99 citation statements)

References 16 publications

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“…In 1997, Boderke et al used CLSM to show that the amino peptidase activity was evenly distributed throughout the viable part of the epidermis, with enhanced fluorescence in the upper layers of the stratum granulosum, while dermis and SC showed considerably less amino peptidase activity (Boderke et al, 1997 demonstrate that vesicles made of native human SC lipids rapidly interact with phosphatidylserine liposomes, weakly with human stratum corneum lipid liposomes and have no effect on PC liposomes (Zellmer et al, 1998). Vardaxis et al (1997) employed CLSM to examine the structure of porcine skin and concluded that it provides valuable additional morphological information of material examined by conventional microscopy for wound healing studies (Vrhovnik et al, 1998). Zellmer et al (1995) reported that neither the vesicles nor the fluorophore N-(lissamine rhodamine B sulfonyl) diacylphophatidylethanolamine (Rho-PE) penetrate into the human skin in a detectable amount.…”

Section: Resultsmentioning

confidence: 99%

Particle size of liposomes influences dermal delivery of substances into skin

Verma

Blume

et al. 2003

International Journal of Pharmaceutics

545

257

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Section: Resultsmentioning

confidence: 99%

Particle size of liposomes influences dermal delivery of substances into skin

Verma

Blume

et al. 2003

International Journal of Pharmaceutics

545

257

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“…snout) and haired regions with a lesser variation between animal species [68]. The value chosen here is more reflective of the haired regions, where published epidermal thicknesses include 60 mm in cattle [33], 30 -100 mm and 70-140 mm in swine [30], and 50 mm in sheep [34]. The nominal value used in this study includes both the living and non-living portions of the epidermis.…”

Section: Discussionmentioning

confidence: 99%

Skin as a potential source of infectious foot and mouth disease aerosols

Dillon

2011

Proc. R. Soc. B.

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This review examines whether exfoliated, virus-infected animal skin cells could be an important source of infectious foot and mouth disease virus (FMDV) aerosols. Infectious material rafting on skin cell aerosols is an established means of transmitting other diseases. The evidence for a similar mechanism for FMDV is: (i) FMDV is trophic for animal skin and FMDV epidermis titres are high, even in macroscopically normal skin; (ii) estimates for FMDV skin cell aerosol emissions appear consistent with measured aerosol emission rates and are orders of magnitude larger than the minimum infectious dose; (iii) the timing of infectious FMDV aerosol emissions is consistent with the timing of high FMDV skin concentrations; (iv) measured FMDV aerosol sizes are consistent with skin cell aerosols; and (v) FMDV stability in natural aerosols is consistent with that expected for skin cell aerosols. While these findings support the hypothesis, this review is insufficient, in and of itself, to prove the hypothesis and specific follow-on experiments are proposed. If this hypothesis is validated, (i) new FMDV detection, management and decontamination approaches could be developed and (ii) the relevance of skin cells to the spread of viral disease may need to be reassessed as skin cells may protect viruses against otherwise adverse environmental conditions.

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“…The vasculature of porcine skin resembles that of humans for the presence of a lower, a mid-dermal and a sub-epidermal plexus (Forbes 1967), although the sub-epidermal vascular plexus is significantly less dense in porcine than in human skin (Vardaxis et al 1997). Porcine skin also has less vasculature around the hair follicles and sebaceous glands vs. human skin (Montagna and Yun 1964).…”

Section: Increased Vascularizationmentioning

confidence: 99%

Cellular mechanisms of skin repair in humans and other mammals

Rittié

2016

J. Cell Commun. Signal.

227

167

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The increased incidence of non-healing skin wounds in developed societies has prompted tremendous research efforts on the complex process known as Bwound healing^. Unfortunately, the weak relevance of modern wound healing research to human health continues to be a matter of concern. This review summarizes the current knowledge of the cellular mechanisms that mediate wound closure in the skin of humans and laboratory animals. The author highlights the anatomical singularities of human skin vs. the skin of other mammals commonly used for wound healing research (i.e. as mice, rats, rabbits, and pigs), and discusses the roles of stem cells, myofibroblasts, and the matrix environment in the repair process. The majority of this review focuses on reepithelialization and wound closure. Other aspects of wound healing (e.g. inflammation, fibrous healing) are referred to when relevant to the main topic. This review aims at providing the reader with a clear understanding of the similarities and differences that have been reported over the past 100 years between the healing of human wounds and that of other mammals.

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Confocal laser scanning microscopy of porcine skin: implications for human wound healing studies

Cited by 147 publications

References 16 publications

Particle size of liposomes influences dermal delivery of substances into skin

Particle size of liposomes influences dermal delivery of substances into skin

Skin as a potential source of infectious foot and mouth disease aerosols

Cellular mechanisms of skin repair in humans and other mammals

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