Confocal laser scanning microscopy in orthopaedic research

Jones, Christopher W.; Smoliński, Dariusz Jan; Keogh, Angus; Kirk, T.B.; Zheng, Minghao

doi:10.1016/j.proghi.2005.02.001

Cited by 58 publications

(50 citation statements)

References 116 publications

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“…We proposed the kinetic model to evaluate the spatial growth of chondrocyte cells in Atelocollagen gels, and demonstrated that the heterogeneity of cell distribution developed due to formation of cell aggregate during the culture (Kino-oka et al 2005b, c). Recently, a confocal laser scanning microscope (CLMS) has enabled to display 3D architecture of tissues with high resolution of spatial imaging (Jadin et al 2005;Jones et al 2005;Xu et al 2006). Therefore, the CLSM technique was applied to the culture performances of chondrocytes to clarify the space-and time-dependent process of cell aggregation in Atelocollagen gels.…”

Section: Methodological Development For Evaluation Of Spatial Cell DImentioning

confidence: 99%

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Quality control of cultured tissues requires tools for quantitative analyses of heterogeneous features developed in manufacturing process

2008

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Tissue engineering and related technology have attracted a great deal of medical attention as promising fields for curing defective tissues in vivo. Nowadays, many companies have been established for supplying the reconstructed grafts of cultured tissues for transplantation. The manufacturing processes generally deals with the handlings of starter cells offered by patients (or donors) as raw materials to cultured tissues as products, requiring the construction of novel ex vivo methodologies based on principles different from conventional processes for chemical and pharmaceutical productions. In addition, the raw materials have heterogeneity depending on the state of patients and location of cell harvests, and the products possess spatial cell distribution in the three dimensional structure. These features request a unique strategy in manufacturing process accompanied with the quality control for raw materials and products. This review article describes the contribution of tissue bankers and biochemical engineers to the quality control of cultured tissues during manufacturing, introducing the advances in methodologies to evaluate spatial heterogeneity of cells (or aggregates) and matrices in cultured tissues.

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Section: Methodological Development For Evaluation Of Spatial Cell DImentioning

confidence: 99%

“…The CLSM, as a tool to observe 3D architecture of cultured tissues, can realize the high resolution imaging with an aid of fluorescence staining (Jones et al 2005). Xu et al (2006) visualized chondrocyte morphology in agarose and alginate gels by preparing 3D images of living cells.…”

Section: Methodological Development For Evaluation Of Spatial Cell DImentioning

confidence: 99%

Quality control of cultured tissues requires tools for quantitative analyses of heterogeneous features developed in manufacturing process

2008

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“…In addition, it has the capability of quantitative image analysis. 23) CLSM has mainly been used for image analysis, including cell recognition 24) and tissue imaging, 25) but quantitative CLSM for pharmacokinetics is rarely reported. In this study, we determined the local concentration of drug in a tumor through the quantitative image analysis of CLSM.…”

Section: Doxorubicin-loaded Zein In Situ Gel For Interstitial Chemothmentioning

confidence: 99%

Doxorubicin-Loaded Zein <i>in Situ</i> Gel for Interstitial Chemotherapy

Cao

Geng

et al. 2012

CHEMICAL & PHARMACEUTICAL BULLETIN

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A novel drug delivery system of doxorubicin (DOX)-loaded Zein in situ gel for interstitial chemotherapy was investigated in this study. The possible mechanisms of drug release were described according to morphological analysis by optical microscopy and scanning electronic microscope (SEM). In vitro and in vivo anti-tumor activity studies showed that DOX-loaded Zein in situ gel was superior to DOX solution. Local pharmacokinetics in tumor tissue was studied by quantitative analysis with confocal laser scanning microscopy (CLSM) combined with microdialysis technology. A pharmacokinetics mathematical model of DOX-loaded Zein in situ gel in tumors was then built.

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“…Once a series of sequential 2D slices is captured at different heights in the specimen, 3D images can be complied providing 3D surface and volume information. LSCM has a number of advantages including having a higher resolution than a standard stylus profiler, requiring minimal sample preparation and being a fast, non-intrusive and versatile instrument for quantitative surface measurement [25][26][27][28][29]. Owning to its advantages, LSCM has been used to capture 3D surface information of specimens in engineering and bio-engineering field [30][31][32].…”

Section: Introductionmentioning

confidence: 99%

A new approach to numerical characterisation of wear particle surfaces in three-dimensions for wear study

Tian

Wang

Peng

et al. 2012

Wear

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In the wear and tear process of synovial joints, wear particles generated and released from articular cartilage within the joints have different surface topology and mechanical property. Three-dimensional (3D) particle images acquired using laser scanning confocal microscopy (LSCM) contain appropriate surface information for quantitatively characterizing the surface topology and changes to seek a further understanding of the wear process and wear features. This paper presents a new attempt on the 3D numerical characterisation of wear particle surfaces using the field and feature parameter sets which are defined in ISO/FDIS 25178-2. Based on the innovative pattern recognition capability, the feature parameters are, for the first time, employed for quantitative analysis of wear debris surface textures. Through performing parameter classification, ANOVA analysis and correlation analysis, typical changing trends of the surface transformation of the wear particles along with the severity of wear conditions and Osteoarthritis (OA) have been observed. Moreover, the feature parameters have shown a significant sensitivity with the wear particle surfaces texture evolution under OA development. A correlation analysis of the numerical analysis results of cartilage surface texture variations and that of their wear particles has been conducted in this study. Key surface descriptors have been determined. Further research is needed to verify the above outcomes using clinic samples.

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Confocal laser scanning microscopy in orthopaedic research

Cited by 58 publications

References 116 publications

Quality control of cultured tissues requires tools for quantitative analyses of heterogeneous features developed in manufacturing process

Quality control of cultured tissues requires tools for quantitative analyses of heterogeneous features developed in manufacturing process

Doxorubicin-Loaded Zein <i>in Situ</i> Gel for Interstitial Chemotherapy

A new approach to numerical characterisation of wear particle surfaces in three-dimensions for wear study

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