Confocal laser microscopic observation of glycocalyx production by Staphylococcus aureus in vitro

Akiyama, Hisanori; Huh, Wook Kang; Fujii, Kazuyasu; Oono, Takashi; Iwatsuki, Keiji

doi:10.1016/s0923-1811(02)00007-5

Cited by 35 publications

(41 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…De Bruyne et al (1999) evaluated the antimicrobial activity of a series of proanthocyanidin dimers and veryfied that the minimum inhibitory concentration was >100 µg/ml for Escherichia coli, P. aeruginosa, Salmonella paratyphi, Enterobacter cloacae, Mycobacterium fortuitum, S. aureus and Candida albicans. Djipa et al (2000) correlated the antibacterial activity demonstrated by Syzygium jambos (Myrtaceae) to its high tannin content, and Akiyama et al (2001) iden-tified tannic acid as a possible adjuvant to antibiotics in the treatment of S. aureus infections. Panizzi et al (2002) observed that fractions of tannins and other phenols ex-tracted from Rubus ulmifolius showed high antimicrobial activity.…”

Section: Discussionmentioning

confidence: 97%

“…Plants rich in tannins are employed in traditional medicine to treat a number of diseases (Haslam, 1996;De Bruyne et al, 1999). Extensive analyses have revealed diverse biological activities of this class of substances (Matsuo et al, 1994;Haslam, 1996;Lee et al, 2000), including their ability to inhibit the growth of microorganisms (Scalbert, 1991;Akiyama et al, 2001;Ferreira et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antibacterial activity of tannins from Psidium guineense Sw. (Myrtaceae)

Rodrigues¹,

Ferreira²,

Mendes³

et al. 2014

J. Med. Plants Res.

View full text Add to dashboard Cite

The aim of this study was to quantify and evaluate the antibacterial activity of tannins extracted from leaves of Psidium guineense, obtained using two different isolation methods, against Staphylococcus aureus and Pseudomonas aeruginosa. The first extraction method was used to isolate condensed tannins (Method A) and the second to isolate mixtures rich in esters of gallic acid and glucose (Method B). Both extraction methods yielded high concentrations of tannins, with 312 and 257 mg/g of dry material obtained using Methods A and B, respectively. These compounds formed halos of growth inhibition in S. aureus and P. aeruginosa cultures. Tannins isolated by method B gave rise to larger inhibition halos than those isolated by method A. Analysis of the antibacterial activity of tannins isolated by method B revealed a minimum inhibitory and bactericidal concentration of 256 μg/ml for S. aureus and 512 μg/ml for P. aeruginosa. These results demonstrate that P. guineense is a promising source of pharmacologically active tannin molecules, and further studies are therefore necessary to determine the toxicity of the plant and ensure its safe use for animal and human health.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Antibacterial activity of tannins from Psidium guineense Sw. (Myrtaceae)

Rodrigues¹,

Ferreira²,

Mendes³

et al. 2014

J. Med. Plants Res.

View full text Add to dashboard Cite

show abstract

“…Cultivation experiments for confocal laser scanning microscope (CLSM) observation: Organisms were grown in the culture medium reported by Akiyama et al [1], that was soybean-casein digest broth (Nihon Pharm., Tokyo, Japan) supplemented with the same amount of normal rabbit plasma (Denka Seiken, Tokyo, Japan). Cultivation experiments were performed following the previous reports [1,9,11,27,30].…”

Section: Methodsmentioning

confidence: 99%

“…Cultivation experiments were performed following the previous reports [1,9,11,27,30]. Briefly, the specimen was placed on a 35-mm culture dish, and 2.0 ml of the culture medium containing bacteria was added.…”

Section: Methodsmentioning

confidence: 99%

Prevention of Biofilm Formation with a Coating of 2-Methacryloyloxyethyl Phosphorylcholine Polymer

et al. 2008

View full text Add to dashboard Cite

ABSTRACT. Device-associated infections are serious complications, and their prevention is an issue of considerable importance. Since biofilms are responsible for these refractory infections, effective methods to inhibit biofilm formation are required. In this investigation, stainless steel plates with and without 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer, i.e., poly (MPC-co-n-butyl methacrylate) (PMB) coating, were incubated in a medium containing bacteria. In the course of incubation, half of the specimens received antibiotics. The specimens were stained for nucleic acid and polysaccharides, and then examined with a confocal laser scanning microscope. The numbers of bacteria on the specimen surfaces were evaluated by an ATP assay. On the surfaces of the specimens without PMB coating, the formation of a biofilm enveloping bacteria was confirmed. The addition of antibiotics did not effectively decrease the number of bacteria. On the other hand, on the surfaces of the specimens with PMB coating, no biofilm formation was observed, and the number of bacteria was significantly decreased. The addition of potent antibiotics further decreased the number of bacteria by 1/100 to 1/1000 times. The PMB coating combined with the validated use of antibiotics might provide a method for the simultaneous achievement of biocompatible surfaces of devices and the prevention of device-associated infections. KEY WORDS: biofilm, biomaterials, device-associated infection, MPC, PMB coating.Device-associated infections are serious complications in patients receiving indwelling catheters. These complications, however, do not limited in such cases; device-associated infections are consistent risk accompanying the usage of any artificial devices. For instance, applications of bone fixation plates to open fractures are contraindicated. Actually, the rate of the infections was reported to be as high as 15% to 18% [23] if an aseptic condition was not maintained during the treatment. Although the microorganisms responsible for these infections are usually indigenous bacteria such as Staphylococcus epidermidis, a difficulty arises as antibacterial agents are not effective against these infections. Once the infections are established, persistent inflammations continue as long as the devices remain in the body.Device-associated infections are caused by bacteria that adhere to the surfaces of internal prosthetic devices and are thought to develop through the following sequence of events [3,7,10,18,22]. Immediately after the implantation of devices into patients, various molecules such as peptides and proteins are adsorbed onto the surfaces of the materials. These molecules mediate the attachment of bacteria. Once the bacteria, e.g., those belonging to the species S. epidermidis, are attached to the surfaces of devices, they proliferate and aggregate there to form biofilms in which the bacteria are enveloped in a thin layer of polysaccharides. The bacteria in the biofilms are protected from the biological defense mechanism, including ph...

show abstract

“…However, more than half of the infectious diseases that affect mildly compromised individuals involve bacterial species that are commensals and are common in our natural environment. [31]. Another study conducted by James and his co-worker, they microscopic evaluated specimens from 50 chronic wounds and 16 acute wounds.…”

Section: Clinical Importance Of Biofilms In Chronic Infectionsmentioning

confidence: 99%

Bacterial Biofilms: A Confrontation to Medical Science

2017

IJSR

View full text Add to dashboard Cite

Biofilms is a consortia of microorganisms in which microorganism adhere to a substratum in an irreversible manner and are enclosed in self -produced matrix of extracellular polymeric substance. Common example of biofilm are dental plaque, device associated infection. Mostly biofilms are infective in nature and can be a source of Nosocomial infection. According to a report published by national institute of Health about 65% of all microbial infections and 80% of all chronic infections are associated with biofilms. The structural anatomy of biofilms protects microbial cells from antimicrobial agents, UV rays. These biofilms act as a barrier; antibiotics cannot reach to these microbes hindering the killing of the microbes thus posing a serious threat to public health. Management of these bacterial biofilms requires an early detection method and development of novel and efficient control measures for treating patients improving patient care and timely management.

show abstract

Confocal laser microscopic observation of glycocalyx production by Staphylococcus aureus in vitro

Cited by 35 publications

References 19 publications

Antibacterial activity of tannins from Psidium guineense Sw. (Myrtaceae)

Antibacterial activity of tannins from Psidium guineense Sw. (Myrtaceae)

Prevention of Biofilm Formation with a Coating of 2-Methacryloyloxyethyl Phosphorylcholine Polymer

Bacterial Biofilms: A Confrontation to Medical Science

Contact Info

Product

Resources

About