Confluent and reticulated papillomatosis (Gougerot-Carteaud) successfully treated with tacalcitol

Ginarte, Manuel; Fabeiro, J. M.; Toribio, Jaime

doi:10.1080/09546630252775216

Cited by 30 publications

(19 citation statements)

References 20 publications

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“…Malassezia species are also involved in the pathogenesis of various dermatoses with global distribution, such as seborrheic dermatitis (SD), atopic dermatitis (AD), and, more recently, psoriasis (PS) [3-6]. A growing number of reports demonstrate the implication of Malassezia yeasts in other skin disorders, including folliculitis, onychomycosis, confluent and reticulated papillomatosis, and neonatal cephalic pustulosis [7-10]. Finally, Malassezia yeasts have been associated with systemic infections and outbreaks in neonatal and immunocompromised adults intensive care units [11,12].…”

Section: Introductionmentioning

confidence: 99%

Distribution of Malassezia species on the skin of patients with atopic dermatitis, psoriasis, and healthy volunteers assessed by conventional and molecular identification methods

et al. 2014

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BackgroundThe Malassezia yeasts which belong to the physiological microflora of human skin have also been implicated in several dermatological disorders, including pityriasis versicolor (PV), atopic dermatitis (AD), and psoriasis (PS). The Malassezia genus has repeatedly been revised and it now accommodates 14 species, all but one being lipid-dependent species. The traditional, phenotype-based identification schemes of Malassezia species are fraught with interpretative ambiguities and inconsistencies, and are thus increasingly being supplemented or replaced by DNA typing methods. The aim of this study was to explore the species composition of Malassezia microflora on the skin of healthy volunteers and patients with AD and PS.MethodsSpecies characterization was performed by conventional, culture-based methods and subsequently molecular techniques: PCR-RFLP and sequencing of the internal transcribed spacer (ITS) 1/2 regions and the D1/D2 domains of the 26S rRNA gene. The Chi-square test and Fisher’s exact test were used for statistical analysis.ResultsMalassezia sympodialis was the predominant species, having been cultured from 29 (82.9%) skin samples collected from 17 out of 18 subjects under the study. Whereas AD patients yielded exclusively M. sympodialis isolates, M. furfur isolates were observed only in PS patients. The isolation of M. sympodialis was statistically more frequent among AD patients and healthy volunteers than among PS patients (P < 0.03). Whether this mirrors any predilection of particular Malassezia species for certain clinical conditions needs to be further evaluated. The overall concordance between phenotypic and molecular methods was quite high (65%), with the discordant results being rather due to the presence of multiple species in a single culture (co-colonization) than true misidentification. All Malassezia isolates were susceptible to cyclopiroxolamine and azole drugs, with M. furfur isolates being somewhat more drug tolerant than other Malassezia species.ConclusionsThis study provides an important insight into the species composition of Malassezia microbiota in human skin. The predominance of M. sympodialis in both normal and pathologic skin, contrasts with other European countries, reporting M. globosa and M. restricta as the most frequently isolated Malassezia species.

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Section: Introductionmentioning

confidence: 99%

Distribution of Malassezia species on the skin of patients with atopic dermatitis, psoriasis, and healthy volunteers assessed by conventional and molecular identification methods

et al. 2014

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“…Malassezia yeasts, since first reported in 1889, are known to be implicated in various diseases, including pityriasis versicolor, seborrheic dermatitis, and Malassezia folliculitis. Recently, there have been a growing number of reports which show the implication of Malassezia yeasts in atopic dermatitis, confluent and reticulated papillomatosis (Gougerot-Carteaud), and Malassezia onychomycosis [3][4][5][6][7][8] . Furthermore the patho- 11 on the basis of molecular biology and by employing interdisciplinary approach of morphology, microstructure analysis, and physiology, and many researches have been done on the yeasts species mainly cultured in Malassezia associated skin diseases.…”

Section: Introductionmentioning

confidence: 99%

The Investigation on the Distribution ofMalasseziaYeasts on the Normal Korean Skin by 26S rDNA PCR-RFLP

Jang

Lim

et al. 2009

Ann Dermatol

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Background: Malassezia yeasts are normal flora of the skin that are discovered in 75∼98% of health subjects, but since its association with various skin disorders have been known, many studies have been conducted in the distribution of the yeasts. Objective: To isolate, identify, and classify Malassezia yeasts from the normal human skin of Koreans by using the rapid and accurate molecular biology method (26S rDNA PCR-RFLP) which overcome the limits of morphological and biochemical methods, and to gather a basic database that will show its relation to various skin diseases. Methods: Malassezia yeasts were cultured from clinically healthy human skin using scrub-wash technique at five sites (forehead, cheek, chest, upper arm, and thigh) and swabbing technique at scalp in 160 participants comprised of 80 males and 80 females aged from 0 to 80. Identification of obtained strains were placed into the one of the 11 species by 26S rDNA PCR-RFLP. Results: An overall positive culture rate was 62.4% (599/960). As shown in the experiment groups by their age, the positive culture rate was the highest (74.2%) in the age 21∼30 and 31∼40 (89/120). In the experiment groups by different body areas, the scalp showed the highest positive culture rate of 90% (144/160). On analysis of 26S rDNA PCR-RFLP, M. globosa was the most predominant species in the age 0∼10 (32.8%), 11∼20 (28.9%), 21∼30 (32.3%). M. restricta was identified as predominant species in the age 41∼50 (27.9%), 61∼70 (31.5%) and 71∼80 (24.0%). In the age 31∼40 years, M. sympodialis was found to be the most common species (24.6%). According to body site, M. restricta was more frequently recovered in the scalp (56.8%), forehead (39.8%) and cheek (24.0%) and while M. globosa was more frequently recovered in the chest (36.8%). Higher positive culture rates of Malassezia yeasts were shown in male subjects than female counterparts in all body areas except scalp (p＜0.05). Especially in this study, M. dermatis, newly isolated Malassezia species from atopic dermatitis patient in Japan, was isolated and identified in 19 cases (1.9%) in healthy subjects. Conclusion: The key is to recognize the existence of a difference in the type of Malassezia species in different ages as well as body areas, which reflects differing skin lipid levels in various ages and different body areas. Moreover, 26S rDNA PCR-RFLP analysis which was opted in this study could provide a sensitive and rapid identification system for Malassezia species, which may be applied to epidemiological surveys and clinical practice.

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“…The yeasts, since being first introduced in 1889, have been linked to various skin conditions such as pityriasis versicolor, seborrheic dermatitis, and Malassezia folliculitis, and most recently, atopic dermatitis3,4. Its pathogenic ability is drawing attention more than ever as cases of confluent and reticulated papillomatosis5,6 and Malassezia onychomycosis, as well as systemic Malassezia infection in immunocompromised adults and neonates receiving intravenous fluid replacement7,8 have recently been reported9,10.…”

Section: Introductionmentioning

confidence: 99%

Molecular Biological Identification ofMalasseziaYeasts Using Pyrosequencing

et al. 2013

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BackgroundA Pyrosequencing assay has been used in identification of fungal species such as Candida or Aspergillus and diagnosis of pathogenic bacteria such as Helicobacter pylori but there has been no report on successful isolation and identification of Malassezia yeasts using the pyrosequencing method.ObjectiveExamine the applicability and plausibility of the pyrosequencing method in identification of the Malassezia species.MethodsAt internal transcribed spacer (ITS) sites 1 and 2, three primers were developed using Pyrosequencing Assay Design Software (Biotage AB). Pyrosequencing was performed on 11 standard strains and 83 genomic DNA samples obtained from 66 healthy controls aged from 1 to 80.ResultsThe eleven Malassezia standard species and 83 genomic DNA samples were successfully identified using the pyrosequencing assay.ConclusionThe pyrosequencing method is a new tool for analysis of Malassezia yeasts, and its precision and rapidity suggests its clinical applicability.

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Confluent and reticulated papillomatosis (Gougerot-Carteaud) successfully treated with tacalcitol

Cited by 30 publications

References 20 publications

Distribution of Malassezia species on the skin of patients with atopic dermatitis, psoriasis, and healthy volunteers assessed by conventional and molecular identification methods

Distribution of Malassezia species on the skin of patients with atopic dermatitis, psoriasis, and healthy volunteers assessed by conventional and molecular identification methods

The Investigation on the Distribution ofMalasseziaYeasts on the Normal Korean Skin by 26S rDNA PCR-RFLP

Molecular Biological Identification ofMalasseziaYeasts Using Pyrosequencing

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