Conflicting mitochondrial and nuclear phylogeographic signals and evolution of host-plant shifts in the boreo-montane leaf beetle Chrysomela lapponica

Mardulyn, Patrick; Othmezouri, Nicolas; Mikhailov, Yu. E.; Pasteels, Jacques

doi:10.1016/j.ympev.2011.09.001

Cited by 24 publications

(20 citation statements)

References 48 publications

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“…Variability in the host chemistry existing among species within the genus Salix (Nyman & Julkunen‐Tiitto, ) could contribute to the differences in the gene expression among WFPs. In combination with the beetles’ genetic distinctiveness over wide geographic distances (Mardulyn et al., ), it is not surprising that the expression of a few chemosensory genes differs between populations over such a large geographic distance, even if they are adapted to the same host genus (but not species). Therefore, future studies are encouraged to construct large‐scale phylogeographic analyses of chemosensory gene expression in relation to host chemistry.…”

Section: Discussionmentioning

confidence: 99%

A subset of chemosensory genes differs between two populations of a specialized leaf beetle after host plant shift

Wang

Pentzold

Kunert

et al. 2018

Ecology and Evolution

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Due to its fundamental role in shaping host selection behavior, we have analyzed the chemosensory repertoire of Chrysomela lapponica. This specialized leaf beetle evolved distinct populations which shifted from the ancestral host plant, willow (Salix sp., Salicaceae), to birch (Betula rotundifolia, Betulaceae). We identified 114 chemosensory candidate genes in adult C. lapponica: 41 olfactory receptors (ORs), eight gustatory receptors, 17 ionotropic receptors, four sensory neuron membrane proteins, 32 odorant binding proteins (OBPs), and 12 chemosensory proteins (CSP) by RNA‐seq. Differential expression analyses in the antennae revealed significant upregulation of one minus‐C OBP (Clap OBP27) and one CSP (Clap CSP12) in the willow feeders. In contrast, one OR (Clap OR17), four minus‐C OBPs (Clap OBP02, 07, 13, 20), and one plus‐C OBP (Clap OBP32) were significantly upregulated in birch feeders. The differential expression pattern in the legs was more complex. To narrow down putative ligands acting as cues for host discrimination, the relative abundance and diversity of volatiles of the two host plant species were analyzed. In addition to salicylaldehyde (willow‐specific), both plant species differed mainly in their emission rate of terpenoids such as (E,E)‐α‐farnesene (high in willow) or 4,8‐dimethylnona‐1,3,7‐triene (high in birch). Qualitatively, the volatiles were similar between willow and birch leaves constituting an “olfactory bridge” for the beetles. Subsequent structural modeling of the three most differentially expressed OBPs and docking studies using 22 host volatiles indicated that ligands bind with varying affinity. We suggest that the evolution of particularly minus‐C OBPs and ORs in C. lapponica facilitated its host plant shift via chemosensation of the phytochemicals from birch as novel host plant.

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Section: Discussionmentioning

confidence: 99%

A subset of chemosensory genes differs between two populations of a specialized leaf beetle after host plant shift

Wang

Pentzold

Kunert

et al. 2018

Ecology and Evolution

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“…This species is adapted to cold temperatures and is currently restricted to fragmented populations at high altitudes or latitudes. DNA sequence variation for two of the gene fragments sequenced in the current study were included in a phylogeographic study of C. lapponica (Mardulyn et al ). A visual inspection of allele networks inferred in both species highlights common features: strong phylogeographic structure is visible among regions (i.e., mountain ranges), suggesting a long period of isolation among them.…”

Section: Discussionmentioning

confidence: 99%

“…The aims of our study were as follows: (1) to characterize phylogeographic structure within C. aeneicollis ; (2) to illustrate the process of deriving spatially explicit coalescence models from distribution models to test historical hypotheses; (3) to test whether gene flow currently occurs among the main regions of the contemporary range of C. aeneicollis , given its current fragmented distribution and to test whether this gene flow occurred during the last ice age or was a more ancient phenomenon. To avoid the inference of a general pattern from a single case study, we also compared the phylogeographic pattern uncovered for C. aeneicollis to that of another cold‐adapted leaf beetle, C. lapponica (Mardulyn et al ), a closely related species sharing many ecological traits with C. aeneicollis (similar life cycle and feeding exclusively on willow and birch) and characterized by a similarly fragmented distribution in Eurasia.…”

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confidence: 99%

Inferring the Past and Present Connectivity Across the Range of a North American Leaf Beetle: Combining Ecological Niche Modeling and a Geographically Explicit Model of Coalescence

et al. 2014

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The leaf beetle Chrysomela aeneicollis occurs across Western North America, either at high elevation or in small, isolated populations along the coast, and thus has a highly fragmented distribution. DNA sequence data (three loci) were collected from five regions across the species range. Population connectivity was examined using traditional ecological niche modeling, which suggested that gene flow could occur among regions now and in the past. We developed geographically explicit coalescence models of sequence evolution that incorporated a two-dimensional representation of the hypothesized ranges suggested by the niche-modeling estimates. We simulated sequence data according to these models and compared them to observed sequences to identify most probable scenarios regarding the migration history of C. aeneicollis. Our results disagreed with initial niche-modeling estimates by clearly rejecting recent connectivity among regions, and were instead most consistent with a long period of range fragmentation, extending well beyond the last glacial maximum. This application of geographically explicit models of coalescence has highlighted some limitations of the use of climatic variables for predicting the present and past range of a species and has explained aspects of the Pleistocene evolutionary history of a cold-adapted organism in Western North America. K E Y W O R D S :Chrysomela aeneicollis, coalescent simulations, leaf beetle, phylogeography, PHYLOGEOSIM, range fragmentation.

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“…The primers used were COI-F (5′-ATAATTTTTTTTATAGTTATACC-3′) and COI2-2 [6] for COI , and 5′-TAATATGGCAGATTAGTGCATTGGA-3′ and 5′-GAGACCATTACTTGCTTTCAGTCATCT-3′ ([56], modified from [57]; see [14] for details) for tRNA Leu –COII . Nuclear EF-1α fragment was amplified using primers 5′-GGTATCACCATTGATATTGCHTTDTGGAA-3′ and 5′-ACCAGCAACATAACCACGACG-3′ [58]. Two nuclear 28S rRNA gene fragments were amplified using the primers, 28S-01 (5′-GACTACCCCCTGAATTTAAGCAT-3′) in combination with 28S-R01 (5′-GACTCCTTGGTCCGTGTTTCAAG-3′) for D2–D3 fragment [59] and 28SD4-5-F in combination with 28SD4-5-R for D4–D5 fragment [60].…”

Section: Methodsmentioning

confidence: 99%

Global Genetic Differentiation in a Cosmopolitan Pest of Stored Beans: Effects of Geography, Host-Plant Usage and Anthropogenic Factors

et al. 2014

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Genetic differentiation can be promoted allopatrically by geographic isolation of populations due to limited dispersal ability and diversification over time or sympatrically through, for example, host-race formation. In crop pests, the trading of crops across the world can lead to intermixing of genetically distinct pest populations. However, our understanding of the importance of allopatric and sympatric genetic differentiation in the face of anthropogenic genetic intermixing is limited. Here, we examined global sequence variation in two mitochondrial and one nuclear genes in the seed beetle Callosobruchus maculatus that uses different legumes as hosts. We analyzed 180 samples from 42 populations of this stored bean pest from tropical and subtropical continents and archipelagos: Africa, the Middle East, South and Southeast Asia, Oceania and South America. For the mitochondrial genes, there was weak but significant genetic differentiation across continents/archipelagos. Further, we found pronounced differentiation among subregions within continents/archipelagos both globally and within Africa but not within Asia. We suggest that multiple introductions into Asia and subsequent intermixing within Asia have generated this pattern. The isolation by distance hypothesis was supported globally (with or without continents controlled) but not when host species was restricted to cowpeas Vigna unguiculata, the ancestral host of C. maculatus. We also document significant among-host differentiation both globally and within Asia, but not within Africa. We failed to reject a scenario of a constant population size in the recent past combined with selective neutrality for the mitochondrial genes. We conclude that mitochondrial DNA differentiation is primarily due to geographic isolation within Africa and to multiple invasions by different alleles, followed by host shifts, within Asia. The weak inter-continental differentiation is most likely due to frequent inter-continental gene flow mediated by human crop trade.

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Conflicting mitochondrial and nuclear phylogeographic signals and evolution of host-plant shifts in the boreo-montane leaf beetle Chrysomela lapponica

Cited by 24 publications

References 48 publications

A subset of chemosensory genes differs between two populations of a specialized leaf beetle after host plant shift

A subset of chemosensory genes differs between two populations of a specialized leaf beetle after host plant shift

Inferring the Past and Present Connectivity Across the Range of a North American Leaf Beetle: Combining Ecological Niche Modeling and a Geographically Explicit Model of Coalescence

Global Genetic Differentiation in a Cosmopolitan Pest of Stored Beans: Effects of Geography, Host-Plant Usage and Anthropogenic Factors

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