Confirming Rickettsia rickettsii as the etiological agent of lethal spotted fever group rickettsiosis in human patients from Espírito Santo state, Brazil

Faccini-Martínez, Álvaro A.; Muñoz-Léal, Sebastián; Acosta, Igor da Cunha Lima; Oliveira, Stefan Vilges de; Duré, Ana Íris de Lima; Cerutti, Crispim; Labruna, Marcelo Bahia

doi:10.1016/j.ttbdis.2018.01.005

Cited by 12 publications

(11 citation statements)

References 21 publications

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“…Previously published reports, based on serological, immunohistochemical, and epidemiological methods, have suggested R. rickettsii as the probable etiological agent of an outbreak in the 1990s (COREY et al, 1993;SPOLIDORIO et al, 2010a). Consequently, we recently confirmed R. rickettsii as the etiological agent of fatal SFG rickettsiosis in human patients from Espírito Santo state between 2015 and 2017 (FACCINI-MARTÍNEZ et al, 2018). The aim to determine the identity of other pathogenic SFG rickettsiae circulating in Espírito Santo prompted the current study.…”

mentioning

confidence: 70%

First molecular detection of Rickettsia sp. strain Atlantic rainforest in Amblyomma ovale ticks from Espírito Santo state, Brazil

Acosta

Luz

Faccini-Martínez

et al. 2018

Rev. Bras. Parasitol. Vet.

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Espírito Santo state (southeastern Brazil) is considered an endemic area for spotted fever group rickettsioses. In February 2017, we received in our laboratory seven unfed Amblyomma ovale adult ticks collected by a farmer from his clothes and body (not attached) during a working day in the rural area of Ibiraçu municipality, Espírito Santo state. By polymerase chain reaction (PCR) analyses, targeting gltA and ompA rickettsial genes, the DNA of Rickettsia was detected in 6/7 (85.7%) A. ovale. In all cases, DNA sequencing of PCR products revealed that consensus sequences of both genes were 100% identical to gltA and ompA corresponding sequences of Rickettsia sp. strain Atlantic rainforest retrieved from GenBank. This study reports the first molecular detection of Rickettsia sp. strain Atlantic rainforest in A. ovale ticks from Espírito Santo state. Our findings indicate a new Brazilian state in the southeast region at risk of human infection with this tick-borne emerging rickettsial agent.

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confidence: 70%

First molecular detection of Rickettsia sp. strain Atlantic rainforest in Amblyomma ovale ticks from Espírito Santo state, Brazil

Acosta

Luz

Faccini-Martínez

et al. 2018

Rev. Bras. Parasitol. Vet.

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“…13 This study adds to the limited literature on TBDs in South America. [12][13][14][15][16][17] Further work is needed to determine carriage of specific TBD agents in tick vectors in Ecuador. Importantly, these findings highlight the need for surveillance studies to detect TBDs and their vectors, as well as the development of novel low-cost diagnostics for various TBDs.…”

Section: Discussionmentioning

confidence: 86%

Detection of Antibodies to Spotted Fever Group Rickettsiae and Arboviral Coinfections in Febrile Individuals in 2014–2015 in Southern Coastal Ecuador

Farovitch

Sippy

Beltrán-Ayala

et al. 2019

The American Journal of Tropical Medicine and Hygiene

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Tick-borne diseases (TBDs) are a growing public health threat and are increasingly identified as the cause of undifferentiated febrile illness. There is a significant gap in our understanding of ticks and their associated pathogens in Ecuador. An arboviral surveillance study allowed us to explore potential exposure to TBDs in febrile subjects. We tested plasma samples from 222 febrile subjects for spotted fever group rickettsial (SFGR) antibodies from southern coastal Ecuador in 2014-2015 via ELISA. Fifty-five (25%) subjects had evidence of anti-SFRG IgG or IgM antibodies. Although attempts to detect Rickettsia species in plasma by polymerase chain reaction were unsuccessful, these preliminary data suggest the possibility of endemic SFGR transmission in Ecuador. To better understand the burden and entomological risk for TBDs in Ecuador, future studies should expand TBD surveillance in humans, document common human-biting ticks, and measure pathogen carriage rates in questing ticks.

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“…Both cases were confirmed as RMSF by molecular methods. ompA gene, as previously described (6). Rickettsial DNA from the samples generated sequences with 100% identity to the corresponding ompA gene fragment of R. rickettsii (GenBank accession no.…”

Section: Research Lettersmentioning

confidence: 99%

“…We tested serum samples collected on July 22 from case-patient A and on August 2 from case-patient B. We used a Rickettsia genus-specific quantitative PCR to amplify rickettsial gltA gene from the patients' serum samples (6). Case-patient A had a cycle threshold value of 25.9 and case-patient B 35.3.…”

mentioning

confidence: 99%

Needlestick-Associated Rocky Mountain Spotted Fever, Brazil

Oliveira¹,

Faccini-Martínez²,

Adelino³

et al. 2020

Emerg. Infect. Dis.

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Confirming Rickettsia rickettsii as the etiological agent of lethal spotted fever group rickettsiosis in human patients from Espírito Santo state, Brazil

Cited by 12 publications

References 21 publications

First molecular detection of Rickettsia sp. strain Atlantic rainforest in Amblyomma ovale ticks from Espírito Santo state, Brazil

First molecular detection of Rickettsia sp. strain Atlantic rainforest in Amblyomma ovale ticks from Espírito Santo state, Brazil

Detection of Antibodies to Spotted Fever Group Rickettsiae and Arboviral Coinfections in Febrile Individuals in 2014–2015 in Southern Coastal Ecuador

Needlestick-Associated Rocky Mountain Spotted Fever, Brazil

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