Confirmation rates of array-CGH in day-3 embryo and blastocyst biopsies for preimplantation genetic screening

Mir, Pere; Mateu, Emilia; Mercader, Amparo; Herrer, R.; Rodrigo, Lorena; Vera, M.; Peinado, Vanessa; Milán-Sánchez, Miguel; Campos‐Galindo, Inmaculada; García-Herrero, Sandra; Simón, Carlos; Rubio, Carmen

doi:10.1007/s10815-015-0605-0

Cited by 16 publications

(9 citation statements)

References 25 publications

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“…Embryo biopsy was performed on d3, although we had previously demonstrated a high correlation between the results obtained on d3 or d5 after biopsy even though the array-CGH protocol for PGS is used [32]. It should also be noted that nowadays our donors are stimulated according to a GnRH antagonist protocol and undergo GnRH triggering in all cases to avoid ovarian hyperstimulation syndrome (OHSS).…”

Section: Discussionmentioning

confidence: 99%

A Higher Ovarian Response after Stimulation for IVF Is Related to a Higher Number of Euploid Embryos

Labarta

Bosch

Mercader

et al. 2017

BioMed Research International

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This study has analysed the relationship between ovarian response and the number of euploid embryos. This is a post hoc analysis of a subset of data generated during a prospective cohort study previously published. Forty-six oocyte donors were subjected to ovarian stimulation with 150 IU of rFSH and 75 IU of hp-hMG in a GnRH agonist long protocol. Preimplantation genetic screening was performed in all viable embryos. We observed a positive relationship between ovarian response and the number of euploid embryos. When ovarian response was above the median (≥17 oocytes), the mean number of euploid embryos per donor was 5.0 ± 2.4, while when <17 oocytes were obtained the mean number of euploid embryos was 2.7 ± 1.4 (p = 0.000). Aneuploidy rate did not increase with ovarian response or gonadotropin doses. Also, the number of euploid embryos was inversely related to the amount of gonadotropins needed per oocyte obtained (ovarian sensitivity index). These results suggest that the number of euploid embryos available for embryo transfer increases as the number of oocytes obtained does. Considering the total number of euploid embryos seems more relevant than the aneuploidy rate.

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Section: Discussionmentioning

confidence: 99%

A Higher Ovarian Response after Stimulation for IVF Is Related to a Higher Number of Euploid Embryos

Labarta

Bosch

Mercader

et al. 2017

BioMed Research International

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“…Such studies have been carried out previously during the evaluation of aCGH. These investigations have demonstrated a good correlation, with little impact of mosaicism on diagnostic accuracy (20)(21)(22)(23).…”

Section: Validation Of Mosaicism Detection With the Use Of Hr-ngsmentioning

confidence: 95%

Detection of mosaicism at blastocyst stage with the use of high-resolution next-generation sequencing

Munné

Wells

2017

Fertility and Sterility

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103

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A significant proportion of human preimplantation embryos produced during the course of in vitro fertilization (IVF) treatments contain two or more cytogenetically distinct cell lines. This phenomenon, known as chromosomal mosaicism, can involve the presence of cells with different types of aneuploidy in the absence of any normal cells or a mixture of euploid and abnormal cells. Although a high prevalence of mosaicism at the cleavage and blastocyst stages has been appreciated for two decades, the precise frequency of the phenomenon and its consequences for embryo viability have been difficult to quantify. Recent advances in genetic technologies, such as high-resolution next-generation sequencing, have allowed mosaicism to be detected with much greater sensitivity than earlier methods. The application of these techniques to trophectoderm biopsies, taken from embryos before transfer to the uterus, has provided insight into the clinical impact of mosaicism. Data from recent studies show that blastocysts associated with mosaic trophectoderm biopsy specimens implant less often than embryos with a chromosomally normal biopsy. In addition, the mosaic embryos that succeed in establishing a pregnancy are at a significantly higher risk of miscarriage. Because mosaic embryos are less likely to produce a viable pregnancy than their euploid counterparts, we suggest that they are given a lower priority for transfer to the uterus. However, because these embryos can sometimes produce successful pregnancies, it is important that they can be considered for transfer in the absence of fully euploid embryos and after appropriate patient counseling. Unlike aneuploidy of meiotic origin, mosaicism, which is caused by mitotic errors occurring after fertilization, does not increase with advancing maternal age. There may, however, be clinical, treatment, or patient-related factors that contribute to the risk of mosaicism occurring. This review discusses the validation of methods that permit the detection of chromosomal mosaicism in IVF embryos and findings of clinical relevance.

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“…Nevertheless, the current knowledge about chromosomal defects in preimplantation embryos explains that meiotic errors imply that all the cells of the embryo carry the same chromosomal error while mitotic errors imply that some cells will have different chromosomal content (mosaic embryos). [ 15 16 17 18 ]…”

Section: Discussionmentioning

confidence: 99%

Morphokinetic behavior of euploid and aneuploid embryos analyzed by time-lapse in embryoscope

Patel¹,

Shah²,

Kotdawala³

et al. 2016

J Hum Reprod Sci

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BACKGROUND:Embryonic aneuploidy may result in miscarriage, implantation failure, or birth defects. Thus, it is clinically necessary to avoid the selection of aneuploid embryos during in vitro fertilization treatment.AIM:The aim of this study was to identify the morphokinetic differences by analyzing the development of euploid and aneuploid embryos using a time-lapse technology. We also checked the accuracy of a previously described model for selection of euploid embryos based on morphokinetics in our study population.MATERIALS AND METHODS:It is a retrospective study of 29 cycles undergoing preimplantation genetic screening from October 2013 to April 2015 at our center. Of 253 embryos, 167 suitable for biopsy embryos were analyzed for their chromosomal status using array-comparative genome hybridization (CGH). The morphokinetic behavior of these embryos was further analyzed in embryoscope using time-lapse technology.RESULTS:Among the analyzed embryos, 41 had normal and 126 had abnormal chromosome content. No significant difference in morphokinetics was found between euploid and aneuploid embryos. The percentage of embryos with blastulation was similar in the euploid (65.85%, 27/41) and aneuploid (60.31%, 76/126) embryos (P = 0.76). Although hard to define, majority of the chromosomal defects might be due to meiotic errors. On applying embryo selection model from Basile et al., embryos falling within optimal ranges for time to division to 5 cells (t5), time period of the third cell cycle (CC3), and time from 2 cell division to 5 cell division (t5-t2) exhibited greater proportion of normal embryos than those falling outside the optimal ranges (28.6%, 25.9%, and 26.7% vs. 17.5%, 20.8%, and 14.3%).CONCLUSION:Keeping a track of time interval between two stages can help us recognize aneuploid embryos at an earlier stage and prevent their selection of transfer. However, it cannot be used as a substitute for array CGH to select euploid embryos for transfer.

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Confirmation rates of array-CGH in day-3 embryo and blastocyst biopsies for preimplantation genetic screening

Cited by 16 publications

References 25 publications

A Higher Ovarian Response after Stimulation for IVF Is Related to a Higher Number of Euploid Embryos

A Higher Ovarian Response after Stimulation for IVF Is Related to a Higher Number of Euploid Embryos

Detection of mosaicism at blastocyst stage with the use of high-resolution next-generation sequencing

Morphokinetic behavior of euploid and aneuploid embryos analyzed by time-lapse in embryoscope

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