Confirmation of QTLs controlling Ascochyta fabae resistance in different generations of faba bean (Vicia faba L.)

Díaz-Ruíz, Ramón; Šatović, Zlatko; Ávila, Carmen M.; Alfaro, Carmen; Gutiérrez, María Victoria; Torres, A. M.; Román, Belén

doi:10.1071/cp08190

Cited by 37 publications

(33 citation statements)

References 41 publications

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“…Typically biparental RIL or F 2 populations generally consisting of no more than 200 progeny lines were genotyped with non-sequence based markers, chiefly RAPD. The highlights of this phase of faba bean genetics were a series of RAPD-based QTL studies targeting resistance to pathogens and parasites—for instance, to ascochyta (Avila et al, 2004 ; Diaz-Ruiz et al, 2009 ), rust (Avila et al, 2003 ), and Orobanche (Diaz-Ruiz et al, 2010 ; Gutierrez et al, 2013 ). This early period of integration of molecular marker technology in genetic mapping studies has been reviewed by Torres et al ( 2010 ) and Gnanasambandam et al ( 2012 ).…”

Section: Geneticsmentioning

confidence: 99%

Advances in Faba Bean Genetics and Genomics

O’Sullivan

Angra

2016

Front. Genet.

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Vicia faba L, is a globally important grain legume whose main centers of diversity are the Fertile Crescent and Mediterranean basin. Because of its small number (six) of exceptionally large and easily observed chromosomes it became a model species for plant cytogenetics the 70s and 80s. It is somewhat ironic therefore, that the emergence of more genomically tractable model plant species such as Arabidopsis and Medicago coincided with a marked decline in genome research on the formerly favored plant cytogenetic model. Thus, as ever higher density molecular marker coverage and dense genetic and even complete genome sequence maps of key crop and model species emerged through the 1990s and early 2000s, genetic and genome knowledge of Vicia faba lagged far behind other grain legumes such as soybean, common bean and pea. However, cheap sequencing technologies have stimulated the production of deep transcriptome coverage from several tissue types and numerous distinct cultivars in recent years. This has permitted the reconstruction of the faba bean meta-transcriptome and has fueled development of extensive sets of Simple Sequence Repeat and Single Nucleotide Polymorphism (SNP) markers. Genetics of faba bean stretches back to the 1930s, but it was not until 1993 that DNA markers were used to construct genetic maps. A series of Random Amplified Polymorphic DNA-based genetic studies mainly targeted at quantitative loci underlying resistance to a series of biotic and abiotic stresses were conducted during the 1990's and early 2000s. More recently, SNP-based genetic maps have permitted chromosome intervals of interest to be aligned to collinear segments of sequenced legume genomes such as the model legume Medicago truncatula, which in turn opens up the possibility for hypotheses on gene content, order and function to be translated from model to crop. Some examples of where knowledge of gene content and function have already been productively exploited are discussed. The bottleneck in associating genes and their functions has therefore moved from locating gene candidates to validating their function and the last part of this review covers mutagenesis and genetic transformation, two complementary routes to validating gene function and unlocking novel trait variation for the improvement of this important grain legume.

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Section: Geneticsmentioning

confidence: 99%

Advances in Faba Bean Genetics and Genomics

O’Sullivan

Angra

2016

Front. Genet.

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“…A large number of intron-targeted primer pairs (ITAPs), developed within the Grain Legumes Integrated Project-GLIP ( http://www.pcgin.org/GLIP/pubrep.pdf ), was tested and mapped in two faba bean inbred populations (Vf6 × Vf136, 29H × Vf136). These were used to validate QTLs underlying broomrape and Ascochyta resistance in different environments and genetic backgrounds [ 15 , 24 - 26 ]. A third RIL population derived from cross Vf6 × Vf27 was used to construct the first exclusively gene-based genetic map in faba bean.…”

Section: Introductionmentioning

confidence: 99%

A reference consensus genetic map for molecular markers and economically important traits in faba bean (Vicia fabaL.)

et al. 2013

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BackgroundFaba bean (Vicia faba L.) is among the earliest domesticated crops from the Near East. Today this legume is a key protein feed and food worldwide and continues to serve an important role in culinary traditions throughout Middle East, Mediterranean region, China and Ethiopia. Adapted to a wide range of soil types, the main faba bean breeding objectives are to improve yield, resistance to biotic and abiotic stresses, seed quality and other agronomic traits. Genomic approaches aimed at enhancing faba bean breeding programs require high-quality genetic linkage maps to facilitate quantitative trait locus analysis and gene tagging for use in a marker-assisted selection. The objective of this study was to construct a reference consensus map in faba bean by joining the information from the most relevant maps reported so far in this crop.ResultsA combination of two approaches, increasing the number of anchor loci in diverse mapping populations and joining the corresponding genetic maps, was used to develop a reference consensus map in faba bean. The map was constructed from three main recombinant inbreed populations derived from four parental lines, incorporates 729 markers and is based on 69 common loci. It spans 4,602 cM with a range from 323 to 1041 loci in six main linkage groups or chromosomes, and an average marker density of one locus every 6 cM. Locus order is generally well maintained between the consensus map and the individual maps.ConclusionWe have constructed a reliable and fairly dense consensus genetic linkage map that will serve as a basis for genomic approaches in faba bean research and breeding. The core map contains a larger number of markers than any previous individual map, covers existing gaps and achieves a wider coverage of the large faba bean genome as a whole. This tool can be used as a reference resource for studies in different genetic backgrounds, and provides a framework for transferring genetic information when using different marker technologies. Combined with syntenic approaches, the consensus map will increase marker density in selected genomic regions and will be useful for future faba bean molecular breeding applications.

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“…The high content in transposable elements 11 complexifies the faba bean genome assembly and map-based cloning. Most of the linkage maps generated so far have a low to medium saturation and are based on morphological, isozyme, restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), sequence characterized amplified region (SCAR), intron targeted amplified polymorphism (ITAP), simple sequence repeat (SSR) and low-density single-nucleotide polymorphism (SNP) markers [12][13][14][15][16][17][18][19][20][21] . SNP-based genetic maps have been recently developed 21 .…”

mentioning

confidence: 99%

Development of new genetic resources for faba bean (Vicia faba L.) breeding through the discovery of gene-based SNP markers and the construction of a high-density consensus map

Carrillo‐Perdomo

Vidal

Kreplak

et al. 2020

Sci Rep

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faba bean (Vicia faba L.) is a pulse crop of high nutritional value and high importance for sustainable agriculture and soil protection. With the objective of identifying gene-based Snps, transcriptome sequencing was performed in order to reduce faba bean genome complexity. A set of 1,819 genebased Snp markers polymorphic in three recombinant line populations was selected to enable the construction of a high-density consensus genetic map encompassing 1,728 markers well distributed in six linkage groups and spanning 1,547.71 cM with an average inter-marker distance of 0.89 cM. orthology-based comparison of the faba bean consensus map with legume genome assemblies highlighted synteny patterns that partly reflected the phylogenetic relationships among species. Solid blocks of macrosynteny were observed between faba bean and the most closely-related sequenced legume species such as pea, barrel medic or chickpea. Numerous blocks could also be identified in more divergent species such as common bean or cowpea. the genetic tools developed in this work can be used in association mapping, genetic diversity, linkage disequilibrium or comparative genomics and provide a backbone for map-based cloning. This will make the identification of candidate genes of interest more efficient and will accelerate marker-assisted selection (MAS) and genomic-assisted breeding (GAB) in faba bean.Legume crops serve as a source of food and feed. They also play an important role in sustainable agriculture because of their ability to improve soil fertility by fixing atmospheric nitrogen and increasing crop yield when used in crop rotation with cereals or intercropping 1 . In particular, faba bean (Vicia faba L.; Vf) is a primary ingredient of daily meals in both developing and industrialized countries due to its high content in proteins, carbohydrates, dietary fibers and micronutrients 2,3 . It is the most yielding pulse crop after field pea. However, its yield is still about half that of wheat, indicating that great breeding efforts are still needed 4 . Faba bean yield is greatly affected by environmental conditions, especially extreme temperatures, drought and acidity 5,6 . In addition, diseases such as chocolate spot (Botrytis fabae S. or B. cinerea P.) or ascochyta blight (Ascochyta fabae S.), viruses such as faba bean necrotic viruses, parasitic weeds of Orobanche genus and pests such as leaf weevil (Sitona lineatus L.), aphids (Aphis fabae S., A. craccivora K., Acyrthosiphon pisum H., Myzus persicae S.) or seed weevils (Bruchus rufimanus B.) considerably reduce its yield and affect the commercialization of the grains 5,7 . Other factors limiting the production of faba bean include the overproduction of flowers resulting in a variable fertilization

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Confirmation of QTLs controlling Ascochyta fabae resistance in different generations of faba bean (Vicia faba L.)

Cited by 37 publications

References 41 publications

Advances in Faba Bean Genetics and Genomics

Advances in Faba Bean Genetics and Genomics

A reference consensus genetic map for molecular markers and economically important traits in faba bean (Vicia fabaL.)

Development of new genetic resources for faba bean (Vicia faba L.) breeding through the discovery of gene-based SNP markers and the construction of a high-density consensus map

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