Confirmation of QTL mapping and marker validation for partial seedling resistance to crown rot in wheat line '2-49'

Collard, B. C. Y.; Jolley, R.; Bovill, William D.; Grams, R. A.; Wildermuth, G. B.; Sutherland, Mark W.

doi:10.1071/ar05419

Cited by 29 publications

(18 citation statements)

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“…Collard et al (2006) have previously validated the 1D QTL (QCr.usq-1D.1) in a Gluyas Early x Janz doubled haploid population. The detection of QCr.usq-1D.1 in two further populations in this current study indicates the consistent seedling expression of this QTL across a range of backgrounds.…”

Section: Resultsmentioning

confidence: 99%

“…Partial resistance to crown rot in wheat is a quantitatively inherited trait showing a continuous distribution in all of the segregating populations that have been examined thus far Collard et al, 2005;Bovill et al, 2006;Collard et al, 2006). Quantitative traits are not easily grouped into distinct categories, as the range of appearance of one genotype often overlaps that of others, creating the appearance of a continuous distribution (Kearsey and Pooni, 1996).…”

Section: Introductionmentioning

confidence: 99%

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Pyramiding QTL increases seedling resistance to crown rot (Fusarium pseudograminearum) of wheat (Triticum aestivum)

et al. 2010

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Crown rot of wheat (Triticum aestivum), predominantly caused by the fungus Fusarium pseudograminearum, has become an increasingly important disease constraint in many winter cereal production regions in Australia. Our group has previously identified a range of quantitative trait loci (QTL) for partial resistance to crown rot in various bread wheat sources. Here we report on work that has assessed the effectiveness of pyramiding QTL to improve resistance to crown rot. Two doubled haploid populations were analysed -one from a cross between two previously characterised sources of partial seedling resistance (2-49 and W21MMT70; n = 208) and one from a cross between 2-49 and the commercial variety Sunco, a source of adult field resistance (n = 134). Both populations were phenotyped for seedling resistance to crown rot. Microsatellite and DArT markers were used to construct whole genome linkage maps for use in composite interval mapping (CIM) to identify QTL. Three QTL were detected in both trials conducted on the 2-49/W21MMT70 population. These were located on chromosomes 1D (QCr.usq-1D.1), 3B (QCr.usq-3B.1) and 7A. QCr.usq-1D.1 and the previously undetected 7A QTL were inherited from 2-49. QCr.usq-3B.1, inherited from W21MMT70, was the most significant of the QTL, explaining up to 40.5 % of the phenotypic variance. Three QTL were identified in multiple trials of the Sunco/2-49 population. These were located on chromosomes 1D (QCr.usq-1D.1), 2B (QCr.usq-2B.2) and 4B (QCr.usq-4B.1). Only QCr.usq-2B.2 was inherited from Sunco. QCr.usq-4B.1 was the most significant of these QTL, explaining up to 19.1 % of the phenotypic variance.In the 2-49/W21MMT70 population several DH lines performed significantly better than either parent, with the best recording an average disease severity rating of only 3.8 % of that scored by the susceptible check cultivar Puseas. These lines represent a new level of seedling crown rot resistance in wheat.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pyramiding QTL increases seedling resistance to crown rot (Fusarium pseudograminearum) of wheat (Triticum aestivum)

et al. 2010

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“…Janz, Kukri, 2-49, Lang, Sunco, Ernie and EGA-Wylie) and US (e.g. Macon) wheat cultivars Chen et al, 2013a,b;Collard et al, 2005Collard et al, , 2006Ma et al, 2010;Martin et al, 2015;Poole et al, 2012;Wallwork et al, 2004; reviewed by Liu and Ogbonnaya, 2015). Qcrs.cpi.3B, one of the strongest FCR QTLs, is derived from Triticum spelta, a wild relative of hexaploid or bread wheat .…”

Section: Qtls and Marker-assisted Selectionmentioning

confidence: 99%

“…However, no cultivar fully resistant or immune to this pathogen is currently available. Partial host resistance identified against F. pseudograminearum behaves as a quantitative trait controlled by many genes that have relatively small effects (Collard et al ., ; Martin et al ., ; reviewed by Liu and Ogbonnaya, ). Environmental factors, such as water stress, also strongly affect the expression of disease symptoms (Cook, ; Papendick and Cook, ).…”

Section: Introductionmentioning

confidence: 98%

Fusarium crown rot caused by Fusarium pseudograminearum in cereal crops: recent progress and future prospects

Kazan

Gardiner

2018

Molecular Plant Pathology

201

158

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Diseases caused by Fusarium pathogens inflict major yield and quality losses on many economically important plant species worldwide, including cereals. Fusarium crown rot (FCR), caused by Fusarium pseudograminearum, is a cereal disease that occurs in many arid and semi-arid cropping regions of the world. In recent years, this disease has become more prevalent, in part as a result of the adoption of moisture-preserving cultural practices, such as minimum tillage and stubble retention. In this pathogen profile, we present a brief overview of recent research efforts that have not only advanced our understanding of the interactions between F. pseudograminearum and cereal hosts, but have also provided new disease management options. For instance, significant progress has been made in the genetic characterization of pathogen populations, the development of new tools for disease prediction, and the identification and pyramiding of loci that confer quantitative resistance to FCR in wheat and barley. In addition, transcriptome analyses have revealed new insights into the processes involved in host defence. Significant progress has also been made in understanding the mechanistic details of the F. pseudograminearum infection process. The sequencing and comparative analyses of the F. pseudograminearum genome have revealed novel virulence factors, possibly acquired through horizontal gene transfer. In addition, a conserved pathogen gene cluster involved in the degradation of wheat defence compounds has been identified, and a role for the trichothecene toxin deoxynivalenol (DON) in pathogen virulence has been reported. Overall, a better understanding of cereal host-F. pseudograminearum interactions will lead to the development of new control options for this increasingly important disease problem. Taxonomy: Fusarium pseudograminearum O'Donnell & Aoki; Kingdom Fungi; Phylum Ascomycota; Subphylum Pezizomycotina; Class Sordariomycetes; Subclass Hypocreomycetidae; Order Hypocreales; Family Nectriaceae; Genus Fusarium. Disease symptoms: Fusarium crown rot caused by F. pseudograminearum is also known as crown rot, foot rot and root rot. Infected seedlings can die before or after emergence. If infected seedlings survive, typical disease symptoms are browning of the coleoptile, subcrown internode, lower leaf sheaths and adjacent stems and nodal tissues; this browning can become evident within a few weeks after planting or throughout plant development. Infected plants may develop white heads with no or shrivelled grains. Disease symptoms are exacerbated under water limitation. Identification and detection: Fusarium pseudograminearum macroconidia usually contain three to five septa (22-60.5 × 2.5-5.5 μm). On potato dextrose agar (PDA), aerial mycelia appear floccose and reddish white, with red or reddish-brown reverse pigmentation. Diagnostic polymerase chain reaction (PCR) tests based on the amplification of the gene encoding translation elongation factor-1a (TEF-1a) have been developed for molecular identification. Host range: All maj...

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“…No completely resistant cultivars currently exist. The older bread wheat cultivars 'Sunco', 'Baxter', 'Lang', 'Gluyas Early', 'Kukri' and '2-49 wheat line' (Gala/Gluyas Early) offer effective partial resistance to CR under Australian field conditions (Wildermuth et al, 1997;Wallwork et al, 2004;Collard et al, 2005a;Bovill et al, 2006;Collard et al, 2006;Bovill et al, 2010). However, while these varieties are both tolerant to CR and produce high grain quality, they are relatively low yielding.…”

Section: Genetic Resistancementioning

confidence: 99%

Accelerating the development of durum wheat adapted to drought and crown rot conditions

Alahmad¹

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Global demand for durum wheat (Triticum turgidum L. ssp. durum) will increase with our dramatic continuous growing population. However, production is facing climatic fluctuations and evolving pests and diseases. In Australia, the industry has encountered many challenges surrounding stable and profitable production. These include the lack of reliable rainfall throughout the production regions resulting in severe droughts, and the susceptibility of durum wheat to crown rot (CR; caused predominantly by Fusarium pseudograminearum). Damage to durum wheat crops caused by CR is exacerbated by water stress. Thus far, due to the limited protection attainable by fungicide application and lack of genetic resistance in elite durum breeding germplasm, it has been recognised that breeding varieties incorporating reduced susceptibility to CR would be the most effective control measure when effectively combined with other management practices. For example, improved root system architecture holds some promise for enhancing access to water in arid and semi-arid production areas, particularly in deep soils with high water-holding capacity. This may be advantageous in CR-affected environments if it enables improved access to water that could simultaneously help to avoid drought and so reduce the impact of CR on yield. Hence, this research aimed to identify and combine multiple desirable physiological traits, or adaptive features, to provide some tolerance to CR. Firstly, to identify genetic regions influencing root growth angle (RGA) and CR tolerance, an integrated screening method was developed for phenotyping root variation and CR tolerance, along with other important traits such as leaf rust and plant height in durum wheat. The method was adapted to speed breeding and provided plant breeders with protocols and tools to apply selection in early generations of population development. Therefore, the selection will result in enriching recombinant inbred lines (RILs) with desirable alleles and reduce the number of years required to combine these traits in elite breeding populations. Secondly, a nested association mapping (NAM) population was developed within 18 months using speed breeding technology, including an F4 generation in the field. NAM population were developed by crossing eight elite lines imported from the International Centre for Agricultural Research in the Dry Areas (ICARDA) with two Australian cultivars. This population generated a significant variation for above-and below-ground traits that could be harnessed and explored using genetic mapping and breeding approaches. To identify key genomic regions associated with RGA, a subset of 393 NAM lines were phenotyped using the 'clear pot' method. Combining RGA data with 2,541 polymorphic DArTseq markers information, a major iii independent QTL (qSRA-6A) on 6A chromosome was identified. Furthermore, haplotype analysis for this QTL identified two major haplotype groups in the population, highlighting the opportunity for combining root traits with above-ground traits to better ...

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Confirmation of QTL mapping and marker validation for partial seedling resistance to crown rot in wheat line '2-49'

Cited by 29 publications

References 28 publications

Pyramiding QTL increases seedling resistance to crown rot (Fusarium pseudograminearum) of wheat (Triticum aestivum)

Pyramiding QTL increases seedling resistance to crown rot (Fusarium pseudograminearum) of wheat (Triticum aestivum)

Fusarium crown rot caused by Fusarium pseudograminearum in cereal crops: recent progress and future prospects

Accelerating the development of durum wheat adapted to drought and crown rot conditions

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