Confirmation of adherence Protein Hemagglutinin sub-unit Pili with MW 49.6 kDa Helicobacter pylori on Mice Gastric Epithelial Cell

Suharsono, Hamong; Hendrayana, Made Agus; Mantik, I Nyoman; Prawiro, Sumarno Reto

doi:10.9790/3008-09275966

Cited by 2 publications

(7 citation statements)

References 8 publications

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“…In other bacteria, it was reported that a sub unit pili protein of Shigella dysentriae and Salmonella typhi with molecular weight of 49, 8 kDA and 48 kDa respectively were hemaglutinin with adhesion properties 15 . With H. pylori it selves, it has just recently published that a sub unit pili 49, 6 kDa protein of this microorganism was found to be immunogenic and immune dominant in vitro 11 . For this, we reasoned that this protein may be a potential candidate for protection against natural H. pylori infections.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, the in vivo biological properties of the sub unit pili 49,6 kDa protein of H. pylori were investigated. It was previously published that this protein could be purified for the in vitro studies which showed its immunogenicity 11 . In animal models, application of this protein mixed with a commercial cholera toxin adjuvant, revealed a significant inhibition against live H. pylori challenge in the mucosal gastric.…”

Section: Discussionmentioning

confidence: 99%

“…A very recent study has demonstrated that this protein was found to be adherence on mice gastric epithelia cells in vitro. Moreover, the attachment of intact H. pylori cells on the purified mice gastric epithelial cells could be protected by the presence of polyclonal antibodies produced against the homologous protein, indicating the protein was dominant and immunogenic 11 .…”

Section: Introductionmentioning

confidence: 99%

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2015

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Peptic ulcers is one of the major gastrointestinal disorder in human being that generally associated with the infection of Helicobacter pylori, a gram-negative microaerophilic bacterium in stomach. It is also linked to the development of the stomach cancer. The aim of this study was to investigate the in vivo properties of the sub-unit pili proteins with molecular weight of about 49,6 kDa in mice. The bacterium was firstly cultured on the plate of TSA-B (Trypticase Soy Agar with 5% Sheep Blood) to prepare the protein of interes using bacterial cutter and SDS-PAGE. The purified protein was used for a vaccine emulsified with commercial cholera toxin and give orally. The immunized mice showed a significant protection against challenge with live H. pylori cells. In contrast, animals that received the 49, 6 kDa protein without adjuvant as well as the negative control with PBS failed to inhibit adherence of the bacteria, as indicated by a severe damages of gastric tissues. This study has indicated that the sub-unit pili proteins trigered the release of protective antibodies againts the microorganism if combined with cholere toxin adjuvant. Further study is required to investigate the biological functions of this protein as a vaccine candidate for protecting the infection by this microorganism in causing gastric ulcers. QUICK RESPONSE CODE

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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2015

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“…The pathogenic properties of these bacteria were associated with its fimbrial adhesion (pili) [11], a protein found on the bacterial cell surface that plays a role as a bacterial virulence factor [12]. In vitro studies using Western blotting analysis, hemagglutination test, adherence inhibition assays, and immunocytochemical staining revealed that the 49.6-kDa subunit pili protein of H. pylori was immunogenic [13]. The ability of this protein in agglutinating mouse erythrocytes indicates that it was hemagglutinin in nature [13].…”

Section: Introductionmentioning

confidence: 99%

“…In vitro studies using Western blotting analysis, hemagglutination test, adherence inhibition assays, and immunocytochemical staining revealed that the 49.6-kDa subunit pili protein of H. pylori was immunogenic [13]. The ability of this protein in agglutinating mouse erythrocytes indicates that it was hemagglutinin in nature [13]. It was also confirmed that the protein possessed adhesion molecules that play a crucial role in the early phase of the pathogenesis when H. pylor i infiltrate into the epithelial cells of gastric [14-16].…”

Section: Introductionmentioning

confidence: 99%

Antigen of 49.6-kDa subunit pili protein of Helicobacter pylori as a potential biomarker for early and rapid detection of the infection

Suharsono

Muttaqin²,

Tenaya³

et al. 2019

Vet World

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Background and Aim: Helicobacter pylori infection has been identified as a major cause of peptic ulcer diseases, including gastric and duodenal ulcers, gastritis, chronic and gastric carcinoma, and even gastric lymphoma. In vitro studies using Western blotting analysis, hemagglutination test, adherence inhibition assays, and immunocytochemical staining revealed that the 49.6-kDa subunit pili protein of H. pylori was considered an immunogenic protein. This study aimed to develop a serological diagnostic test using 49.6 kDa for detecting antibodies against H. pylori proteins in an early phase of the infection. Materials and Methods: An in-house immunochromatographic test (ICT) kit was developed and used to test a panel of sera sample obtained from a randomly selected symptomatic patient, in which 40 sera were H. pylori positive and 40 sera were H. pylori negative. Results: The results showed that ICT with 49.6 kDa as an antigen was highly sensitive and specific for detecting anti-H. pylori immunoglobulin G antibodies in human serum, with a high negative predictive value. Conclusion: The developed test could be used to exclude H. pylori infection in symptomatic patients.

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Confirmation of adherence Protein Hemagglutinin sub-unit Pili with MW 49.6 kDa Helicobacter pylori on Mice Gastric Epithelial Cell

Abstract: Helicobacter pylori (H.pylori) is a gram-negative microaerophilic bacterium that is

Cited by 2 publications

References 8 publications

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Antigen of 49.6-kDa subunit pili protein of Helicobacter pylori as a potential biomarker for early and rapid detection of the infection

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