Target skyrmion, characterized by a central skyrmion surrounded by a series of concentric cylinder domains known as kπ-skyrmions (k ≥ 2), holds promise as a novel storage state in next-generation memories. However, target skyrmions comprising one or more concentric cylindrical domains have not been observed in chiral magnets, particularly at room temperature. In this study, we experimentally achieved kπ-skyrmions (k = 2, 3, and 4) with diameters of ∼220, 320, and 410 nm, respectively, and room-temperature stability under zero magnetic field by tightly confining these topological spin textures in β-Mn-type Co 8 Zn 10 Mn 2 nanodisks. The magnetic configurations and their field-driven evolutions were simultaneously investigated by using in situ off-axis electron holography. In combination with numerical simulations, we further investigated the dependence of k max on the nanodisk diameter. These findings highlight the potential of kπ-skyrmions as information carriers and offer insights into manipulation of kπ-skyrmions in the future.