Configurable microfluidic platform for investigating therapeutic delivery from biomedical device coatings

Li, Zidong; Şeker, Erkin

doi:10.1039/c7lc00851a

Cited by 11 publications

(16 citation statements)

References 36 publications

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“…Still, PDMS presents other complications; the soft lithography processes used to manufacture PDMS-based MPSs are not suitable for mass production, because the elasticity of the PDMS microfluidic structures renders them difficult to handle [ 17 ], and PDMS-based MPSs cannot be stored for long durations because PDMS undergoes gradual crosslinking, resulting in structural shrinkage [ 13 ]. Alternatives to PDMS include glass [ 18 , 19 ] and silicon [ 20 ], which have long been used as structural materials for microfluidic devices. However, the fabrication of glass- and silicon-based MPSs often requires harsh reagents; glass-based microfluidic devices are very fragile and not suitable for mass production; and silicon-based microfluidic devices often use photoresist materials, which might have unexpected effects on cultured cells [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Effects of Solvents Used in the Fabrication of Microfluidic Devices on Cell Cultures

Wen

Takahashi²,

Hatakeyama³

et al. 2021

Micromachines

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Microfluidic microphysiological systems (MPSs) or “organs-on-a-chip” are a promising alternative to animal models for drug screening and toxicology tests. However, most microfluidic devices employ polydimethylsiloxane (PDMS) as the structural material; and this has several drawbacks. Cyclo-olefin polymers (COPs) are more advantageous than PDMS and other thermoplastic materials because of their low drug absorption and autofluorescence. However, most COP-based microfluidic devices are fabricated by solvent bonding of the constituent parts. Notably, the remnant solvent can affect the cultured cells. This study employed a photobonding process with vacuum ultraviolet (VUV) light to fabricate microfluidic devices without using any solvent and compared their performance with that of solvent-bonded systems (using cyclohexane, dichloromethane, or toluene as the solvent) to investigate the effects of residual solvent on cell cultures. Quantitative immunofluorescence assays indicated that the coating efficiencies of extracellular matrix proteins (e.g., Matrigel and collagen I) were lower in solvent-bonded COP devices than those in VUV-bonded devices. Furthermore, the cytotoxicity of the systems was evaluated using SH-SY5Y neuroblastoma cells, and increased apoptosis was observed in the solvent-processed devices. These results provide insights into the effects of solvents used during the fabrication of microfluidic devices and can help prevent undesirable reactions and establish good manufacturing practices.

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Section: Introductionmentioning

confidence: 99%

Evaluation of the Effects of Solvents Used in the Fabrication of Microfluidic Devices on Cell Cultures

Wen

Takahashi²,

Hatakeyama³

et al. 2021

Micromachines

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“…Fluid transport in pores a few nanometers across is of relevance in many natural and technological processes [1][2] [3][4] [5], ranging from water transport in soils [6] [7], plants [8] [9] and biomembranes [10] to water filtration, catalysis [11], print [12] and Lab-on-a-Chip [13] [14] technologies. It is also of increasing importance in the synthesis of hybrid materials [15], [16] [17] by meltinfiltration [18].…”

Section: Introductionmentioning

confidence: 99%

Capillarity-Driven Oil Flow in Nanopores: Darcy Scale Analysis of Lucas–Washburn Imbibition Dynamics

Gruener

Huber

2018

Transp Porous Med

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We present gravimetrical and optical imaging experiments on the capillarity-driven imbibition of silicone oils in monolithic silica glasses traversed by 3D networks of pores (mesoporous Vycor glass with 6.5 nm or 10 nm pore diameters). As evidenced by a robust square-root-of-time Lucas-Washburn (L-W) filling kinetics, the capillary rise is governed by a balance of capillarity and viscous drag forces in the absence of inertia and gravitational effects over the entire experimental times studied, ranging from a few seconds up to 10 days. A video on the infiltration process corroborates a collective pore filling as well as pronounced imbibition front broadening resulting from the capillarity and permeability disorder, typical of Vycor glasses. The transport process is analyzed within a Darcy scale description, considering a generalized pre-factor of the L-W law, termed Lucas-Washburn-Darcy imbibition ability. It assumes a Hagen-Poiseuille velocity profile in the pores and depends on the porosity, the mean pore diameter, the tortuosity and the velocity slip length and thus on the effective hydraulic pore diameter. For both matrices a reduced imbibition speed and thus reduced imbibition ability, compared to the one assuming the nominal pore diameter, bulk fluidity and bulk capillarity, can be quantitatively traced to an immobile, pore-wall adsorbed boundary layer of 1.4 nm thickness. Presumably, it consists of a monolayer of water molecules adsorbed on the hydrophilic pore walls covered by a monolayer of flat-laying silicone oil molecules. Our study highlights the importance of immobile nanoscopic boundary layers on the flow in tight oil reservoirs as well as the validity of the Darcy scale description for transport in mesoporous media.

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“…The np-Au MEAs ( Figure A2 B) were fabricated using a hybrid approach that merges rapid prototyping techniques [ 28 ] and corrosion-driven nanostructure self-assembly [ 29 ]. Briefly, glass microscope slides were piranha-cleaned followed by sputter-deposition of a stack of blanket metal layers (chrome adhesion layer, gold seed layer and gold-silver alloy layer) as described previously [ 16 ] and similar to how the samples for macro-scale electrochemical cell have been fabricated.…”

Section: Methodsmentioning

confidence: 99%

Electrically Guided DNA Immobilization and Multiplexed DNA Detection with Nanoporous Gold Electrodes

Veselinović

Daggumati

et al. 2018

Nanomaterials

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Molecular diagnostics have significantly advanced the early detection of diseases, where the electrochemical sensing of biomarkers (e.g., DNA, RNA, proteins) using multiple electrode arrays (MEAs) has shown considerable promise. Nanostructuring the electrode surface results in higher surface coverage of capture probes and more favorable orientation, as well as transport phenomena unique to nanoscale, ultimately leading to enhanced sensor performance. The central goal of this study is to investigate the influence of electrode nanostructure on electrically-guided immobilization of DNA probes for nucleic acid detection in a multiplexed format. To that end, we used nanoporous gold (np-Au) electrodes that reduced the limit of detection (LOD) for DNA targets by two orders of magnitude compared to their planar counterparts, where the LOD was further improved by an additional order of magnitude after reducing the electrode diameter. The reduced electrode diameter also made it possible to create a np-Au MEA encapsulated in a microfluidic channel. The electro-grafting reduced the necessary incubation time to immobilize DNA probes into the porous electrodes down to 10 min (25-fold reduction compared to passive immobilization) and allowed for grafting a different DNA probe sequence onto each electrode in the array. The resulting platform was successfully used for the multiplexed detection of three different biomarker genes relevant to breast cancer diagnosis.

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Configurable microfluidic platform for investigating therapeutic delivery from biomedical device coatings

Cited by 11 publications

References 36 publications

Evaluation of the Effects of Solvents Used in the Fabrication of Microfluidic Devices on Cell Cultures

Evaluation of the Effects of Solvents Used in the Fabrication of Microfluidic Devices on Cell Cultures

Capillarity-Driven Oil Flow in Nanopores: Darcy Scale Analysis of Lucas–Washburn Imbibition Dynamics

Electrically Guided DNA Immobilization and Multiplexed DNA Detection with Nanoporous Gold Electrodes

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