Conference Report: NIST WORKSHOP ON LUMINESCENCE STANDARDS FOR CHEMICAL ANALYSIS, Gaithersburg, MD, September 8-9, 1999

Characterization of Standard Reference Material 2940, Mn-ion-doped glass, spectral correction standard for fluorescence

“…Desirable characteristics of artifact standards for fluorescence have been specified [1,[12][13][14] and metal-ion-doped glasses have been suggested as fluorescence standards previously [12,15,16]. Many of the radiometric characteristics of glasses can influence their effectiveness as standards, including absorbance, fluorescence anisotropy, temperature dependence of fluorescence intensity, fluorescence lifetime and photostability [8].…”

“…The increasing use of fluorescence detection in quantitative assays has resulted in a greater need for fluorescence standards [1][2][3][4], particularly those suitable for day-to-day instrument performance validation and spectral correction of emission. NIST has certified Standard Reference Materials (SRMs) 2940 [5] and 2941 [6] for relative spectral correction of emission and recommended them for use as day-to-day intensity standards for instrument performance validation, due to their resistance to photodegradation.…”

Characterization of Standard Reference Material 2941, uranyl-ion-doped glass, spectral correction standard for fluorescence

“…Desirable characteristics of artifact standards for fluorescence have been specified previously [1,[8][9][10] and include that they be photostable, homogeneous, temperature and polarization independent, low concentration, traceable to a primary standard, supplied with certified values and uncertainties, have a large Stokes shift and a long shelf life. Uranium-containing materials and glasses have played important roles in the understanding of fluorescence and the development of measurement techniques based on fluorescence [11][12][13][14][15][16][17][18][19].…”

“…Many of these assays are being used in areas, such as clinical diagnostics, environmental monitoring, and drug development, where regulatory requirements are strict and may require standards for instrument qualification and method validation. The fluorescence community has expressed increasing interest in fluorescence standards at workshops [1][2][3][4] and through questionnaires, particularly for reference materials suitable for relative spectral correction of emission and for performance validation of fluorescence intensity. In response to these needs, National Institute of Standards and Technology (NIST) first qualified a fluorescence spectrometer [5] to make such measurements and is now employing it to certify the fluorescence spectra of Standard Reference Materials (SRMs).…”

Fluorescence standards: Classification, terminology, and recommendations on their selection, use, and production (IUPAC Technical Report)

“…The last of these is closely related to the dependence of the spectroscopic properties of most chromophores (such as absorption and emission spectra, molar absorption coefficient, luminescence quantum yield, luminescence lifetime, and luminescence polarization or anisotropy) on their microenvironment (in terms of temperature, viscosity, solvation, polarity, proticity, pH, ionic strength, presence of quenchers, and attachment to bio-or macromolecules). This situation is further complicated by the existence of very few guidelines, recommendations, and technical notes for the characterization and performance validation of photoluminescence measuring instruments [20][21][22][23][24][25][26][27][28] and for the performance of measurements of relevant photoluminescence quantities [29]. Moreover, concepts need to be developed, evaluated fluorescence standards need to be made available, and relevant fluorometric quantities (e.g., photoluminescence quantum yield) need to be determined to improve the reliability of quantitative fluorescence analyses [1,2,6,7].…”