Cone beam x-ray luminescence computed tomography reconstruction with a priori anatomical information

Lo, Pei An; Lin, Meng Lung; Jin, Shih Chun; Chen, Jyh Cheng; Lin, Syue Liang; Chang, C. Allen; Chiang, Huihua Kenny

doi:10.1117/12.2061321

Cited by 5 publications

(5 citation statements)

References 9 publications

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“…We are in the process of understanding the problem in more detail and will try to optimize the situation at a later stage for practical medical applications. For the initial X-ray-induced optical imaging study, a homemade fluorescence/computed tomography (FT–CT) instrument was used Figure b shows that the core–shell–shell ScNPs have luminescence properties similar to that of NaGdF 4 :Eu, and reasonably good imaging signals could be obtained within 30 s.…”

Section: Resultsmentioning

confidence: 99%

“…For the initial X-ray-induced optical imaging study, a homemade fluorescence/computed tomography (FT−CT) instrument was used. 41 Figure 5b shows that the core−shell−shell ScNPs have luminescence properties similar to that of NaGdF 4 :Eu, 16 and reasonably good imaging signals could be obtained within 30 s.…”

Section: T H Imentioning

confidence: 89%

“…Note that the Eu 3+ emission at 695 nm is closer to the NIR region and might be advantageous for optical imaging to improve the penetration depth. Thus, although the resolution and signal-to-noise ratios of our X-ray-excited FT−CT instrument and image reconstruction software need further improvement, 41 we believe that we have successfully demonstrated that it is possible to use a single X-ray excitation source and well-designed nanocomposites such as ours to perform both PDT (vide infra) and luminescence imaging at the proof-of-principle stage.…”

Section: T H Imentioning

confidence: 93%

See 2 more Smart Citations

Lanthanide-Doped Core–Shell–Shell Nanocomposite for Dual Photodynamic Therapy and Luminescence Imaging by a Single X-ray Excitation Source

Hsu

Lin²,

Chang

2018

ACS Appl. Mater. Interfaces

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Photodynamic therapy (PDT) could be highly selective and noninvasive, with low side effects as an adjuvant therapy for cancer treatment. Because excitation sources such as UV and visible lights for most of the photosensitizers do not penetrate deeply enough into biological tissues, PDT is useful only when the lesions are located within 10 mm below the skin. In addition, there is no prior example of theranostics capable of both PDT and imaging with a single deep-penetrating X-ray excitation source. Here we report a new theranostic scintillator nanoparticle (ScNP) composite in a core-shell-shell arrangement, that is, NaLuF:Gd(35%),Eu(15%)@NaLuF:Gd(40%)@NaLuF:Gd(35%),Tb(15%), which is capable of being excited by a single X-ray radiation source to allow potentially deep tissue PDT and optical imaging with a low dark cytotoxicity and effective photocytotoxicity. With the X-ray excitation, the ScNPs can emit visible light at 543 nm (from Tb) to stimulate the loaded rose bengal (RB) photosensitizer and cause death of efficient MDA-MB-231 and MCF-7 cancer cells. The ScNPs can also emit light at 614 and 695 nm (from Eu) for luminescence imaging. The middle shell in the core-shell-shell ScNPs is unique to separate the Eu in the core and the Tb in the outer shell to prevent resonance quenching between them and to result in good PDT efficiency. Also, it was demonstrated that although the addition of a mesoporous SiO layer resulted in the transfer of 82.7% fluorescence resonance energy between Tb and RB, the subsequent conversion of the energy from RB to generate O was hampered, although the loaded amount of the RB was almost twice that without the mSiO layer. A unique method to compare the wt % and mol % compositions calculated by using the morphological transmission electron microscope images and the inductively coupled plasma elemental analysis data of the core, core-shell, and core-shell-shell ScNPs is also introduced.

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Section: Resultsmentioning

confidence: 99%

Section: T H Imentioning

confidence: 89%

Section: T H Imentioning

confidence: 93%

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Lanthanide-Doped Core–Shell–Shell Nanocomposite for Dual Photodynamic Therapy and Luminescence Imaging by a Single X-ray Excitation Source

Hsu

Lin²,

Chang

2018

ACS Appl. Mater. Interfaces

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“…Through numerical simulations and physical model experiments, the authors demonstrated the effectiveness of the CB-XLCT method and its potential for molecular imaging. Since then, the development of XLCT systems has branched into two types: the first includes the narrow-beam PB-XLCT (Pratx et al 2010b, Pratx et al 2010a and NB (narrow beams)-XLCT (Liu et al 2013), while the second type is CB-XLCT [3, 10-12] (Chen et al 2013, Lo et al 2014. Based on the x-ray emission mode, PB-XLCT and NB-XLCT techniques can achieve high spatial resolution.…”

Section: Introductionmentioning

confidence: 99%

Cone-beam x-ray luminescence computed tomography (CB-XLCT) prototype development and performance evaluation

Wang,

Jin,

et al. 2024

Phys. Med. Biol.

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This study developed a prototype of a rotational cone-beam X-ray luminescence computed tomography (CB-XLCT) system, considering its potential application in pre-clinical theranostic imaging. A geometric calibration method applicable to both imaging chains (XL and CT) was also developed to enhance image quality. The results of systematic performance evaluations were presented to assess the feasibility of commercializing XLCT technology. In terms of development, we first used Monte Carlo GATE simulation to determine the optimal imaging conditions for nanophosphor particles (NPs). Based on the simulation results and the imaging conditions of mice, we acquired a low-dose transmission X-ray tube and designed a prone positioning platform and a rotating gantry, taking reference from commercial small animal μ-CT systems. We then employed image cross-correlation (ICC) automatic geometric calibration method to calibrate XL and CT images. The performance of the system was evaluated through a series of phantom experiments. The CB-XLCT prototype demonstrated a linearity performance of 0.99 in CT images, a contrast of 19.5, and a spatial resolution of 0.077 mm. The XL images of the CB-XLCT prototype achieved a Dice similarity coefficient (DICE) of 0.149 and a peak signal-noise-ratio (PSNR) of 46.897 for a distance of 1 mm between the two light sources. Finally, the final XLCT imaging results were demonstrated using letter phantoms with NPs. In summary, the CB-XLCT prototype developed in this study showed the potential to achieve high-quality imaging with acceptable radiation doses for small animals. The performance of CT images was comparable to current commercial machines, while the XL images exhibited promising results in phantom imaging, but further efforts are needed for biomedical applications.

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“…As the signal of XFCT is independent from background tissues, the concentration distribution of the target element obtained by XFCT has lower background noise and higher contrast than the traditional transmission CT [1,2]. Therefore, XFCT has been considered as a promising approach for applications of imaging of certain elements with low concentration, such as in vivo imaging of nanoparticle probes [3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Experimental Demonstration of X-Ray Fluorescence CT Using a Spatially Distributed Multi-Beam X-Ray Source

Chen

Zhang

2020

Front. Phys.

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X-ray fluorescence computed tomography (XFCT) is a high-sensitivity imaging modality for high-atomic elements such as gadolinium (Gd) or gold (Au). In order to improve the contrast of x-ray fluorescence (XRF) signals in the raw projection data, common XFCT systems use single-pixel x-ray spectrometers to record XRF photons stimulated by the pencil-beam x-ray source and achieve line-by-line scan of the whole object by translating the x-ray source or the object. However, this kind of design results in waiting time of the translation device. One improvement would be to replace the traditional x-ray tube with a spatially distributed multi-beam x-ray source and scan the whole object by switching the exposure of each focus. In this study, we present a design of the XFCT system using a spatially distributed multi-beam source, and a XFCT imaging experiment was performed to investigate its feasibility. Each cold cathode of the x-ray source used in this study was made of carbon nanotubes, and each focus was independently controlled by the electronic control system. The incident beam was collimated by a pinhole array to produce a pencil-beam source. The object scanned by the system was a polymethyl methacrylate (PMMA) cylinder (8 cm in diameter) with Gd (20 mg/ml) and I (100 mg/ml) insertions. Results show that the distribution of iodine (I) and Gd in a PMMA phantom was successfully reconstructed, but the imaging performance was limited by the collimation and exposure mode of the current distributed x-ray source. The practicality of the current distributed x-ray source used for XFCT scan and further optimization of the proposed system are discussed according to the experimental results.

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Cone beam x-ray luminescence computed tomography reconstruction with a priori anatomical information

Cited by 5 publications

References 9 publications

Lanthanide-Doped Core–Shell–Shell Nanocomposite for Dual Photodynamic Therapy and Luminescence Imaging by a Single X-ray Excitation Source

Lanthanide-Doped Core–Shell–Shell Nanocomposite for Dual Photodynamic Therapy and Luminescence Imaging by a Single X-ray Excitation Source

Cone-beam x-ray luminescence computed tomography (CB-XLCT) prototype development and performance evaluation

Experimental Demonstration of X-Ray Fluorescence CT Using a Spatially Distributed Multi-Beam X-Ray Source

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