Cone-beam computerized tomographic, radiographic, and histologic evaluation of periapical repair in dogs' post-endodontic treatment

Paula-Silva, Francisco Wanderley Garcia; Júnior, Milton Santamaria; Leonardo, Mário Roberto; Consolaro, Alberto; Silva, Léa Assed Bezerra da

doi:10.1016/j.tripleo.2009.06.016

Cited by 87 publications

(74 citation statements)

References 31 publications

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“…A model of this endodontic infection could be valuable for improving the understanding of disease etiology and progression and effective treatments. There are many reports regarding the induction of periapical lesions and their histological observation in animals including rats (23,24), dogs (25), cats (26), and monkeys (27). Rat models are the most frequently used models for such studies: the morphology of a rat molar is similar to that of a human molar, and the genetic background of these animals is clear, with practically no individual specificity (28).…”

Section: Discussionmentioning

confidence: 99%

Promotion of Endodontic Lesions in Rats by a Novel Extraradicular Biofilm Model Using Obturation Materials

Kuremoto

Noiri

Ishimoto

et al. 2014

Appl Environ Microbiol

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bAlthough extraradicular biofilm formation is related to refractory periapical periodontitis, the mechanism of extraradicular biofilm development, as well as its effect on periapical lesions, is unknown. Therefore, we aimed to develop an in vivo extraradicular biofilm model in rats and to identify and quantify extraradicular biofilm-forming bacteria while investigating the effect of extraradicular biofilms on periapical lesions. Periapical lesions were induced by exposing the pulpal tissue of the mandibular first molars of male Wistar rats to their oral environment. Four weeks later, gutta-percha points were excessively inserted into the mesial root canals of the right first molars (experimental sites) but not the left first molars (control sites). After 6 and 8 weeks of pulp exposure, the presence of extraradicular biofilms was confirmed histomorphologically, and biofilm-forming bacteria were identified by using classical culture methods. The biofilms were observed in the extraradicular area of the experimental sites. Similar species were detected both inside and outside the root canals. The bacterial count, quantified by real-time PCR assays, in the extraradicular area gradually increased in the experimental sites until 20 weeks after pulp exposure. After 8 weeks of pulp exposure, the periapical lesion volume that was measured by micro-computed tomography was significantly larger in the experimental sites than in the control sites (P < 0.05 by Welch's t test). These results suggest that we developed an extraradicular biofilm model in rats and that extraradicular biofilms affect developing periapical lesions. In clinical practice, we encounter patients in whom periapical periodontitis does not heal despite a root canal treatment following general dental procedures. The etiology is considered to be the result of residual bacteria in the lateral branch of the root canal, endodontic reinfection, or both. Recent studies have shown that biofilms that form outside the apical foramen, which are termed "extraradicular biofilm," are also involved in refractory periapical periodontitis (1-4). As the bacteria in biofilms are resistant to the host's immunoreaction and to antibiotics (5), dental biofilm diseases such as caries or periodontitis have been treated by mechanical removal. However, extraradicular biofilm is impossible to remove mechanically with nonsurgical endodontic treatments. As extraradicular biofilms cannot be accessed from the root canal for mechanical removal, the only treatment methods are endodontic surgery and tooth extraction.Current research is focused on developing methods to prevent or control biofilm formation (6, 7). We recently found three analogues of N-acyl homoserine lactone that participate in quorum sensing, a system of bacterial cell-to-cell communication, and inhibit Porphyromonas gingivalis biofilm formation (8). Furthermore, azithromycin, a 15-membered macrolide antibiotic, has an antibiofilm effect on P. gingivalis (9). For clinical applicability, these in vitro effects should be exami...

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Section: Discussionmentioning

confidence: 99%

Promotion of Endodontic Lesions in Rats by a Novel Extraradicular Biofilm Model Using Obturation Materials

Kuremoto

Noiri

Ishimoto

et al. 2014

Appl Environ Microbiol

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“…56,57 This lesion is commonly caused by bone resorption triggered by the host response to bacteria inside the root canal system. 58 A periapical lesion is defined as periapical radiolucency connected with the apical part of a root that exceeds at least twice the width of the periodontal ligament space. 59,60 Bender and Seltzer 61,62 and Schwarz and Foster 63 showed that the size of the periapical lesion is often underestimated using PRs.…”

Section: Evaluation Of Root Canal Anatomy and Complex Morphologymentioning

confidence: 99%

“…77,78 The most recent literature demonstrates that the detection of periapical lesions following root canal treatment using CBCT is more accurate than that using radiographic evaluation. 68,79,80,67 de Paula-Silva et al 58 evaluated periapical repair after root canal treatment in dogs' teeth using CBCTand PRs and compared these findings with the gold standard: microscopic evaluation. Six months after treatment, a favourable outcome was detected in 79% of teeth assessed with PRs, in comparison to 35% when CBCTwas used.…”

Section: Assessment Of the Outcome Of Root Canal Treatmentmentioning

confidence: 99%

Endodontic applications of cone beam computed tomography: case series and literature review

Abella

Morales

Garrido

et al. 2015

Giornale Italiano di Endodonzia

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Cone beam computed tomography (CBCT) is a relatively new method that produces three-dimensional (3D) information of the maxillofacial skeleton, including the teeth and their surrounding tissue, with a lower effective radiation dose than traditional CT scans. Specific endodontic applications for CBCTare being identified as the use of this technology becomes more common. CBCT has great potential to become a valuable tool for diagnosing and managing endodontic problems, as well as for assessing root fractures, apical periodontitis, resorptions, perforations, root canal anatomy and the nature of the alveolar bone topography around teeth. This article aims to review cone beam technology and its advantages over CT scans and conventional radiography, to illustrate current and future clinical applications in endodontic practice, and to highlight areas of further research of CBCT in endodontics. Specific case examples illustrate how treatment planning has changed with the images obtained with CBCT technology compared with only periapical radiography.

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“…Do ponto de vista tomográfico, estudo efetuado em animais realizado por de Paula- Silva et al, (2009a) evidenciou que a avaliação do reparo pós-tratamento endodôntico por TCFC apresenta resultados similares às informações obtidas por meio de análise histopatológica (padrão ouro), enquanto a análise radiográfica subestima o tamanho das lesões periapicais. No entanto, a comparação dos resultados do presente estudo com a literatura específica tornase impossibilitada pela ausência, até o momento, de trabalhos publicados comparando, em pacientes, a resposta pós-tratamento endodôntico em sessão única ou com o uso de curativo de demora entre sessões, empregando avaliação tomográfica.…”

Section: Discussionunclassified

“…A fim de minimizar as limitações na detecção de lesões intraósseas das radiografias periapicais, estudos recentes têm empregado a Tomografia Computadorizada de Feixe Cônico (TCFC) para aumentar a acurácia no diagnóstico de lesões periapicais (Huumonen et al, 2006;Stavropoulos e Wenzel, 2007;Aggarwal et al, 2008;de Paula-Silva et al, 2009a;Patel et al, 2012a;Patel et al, 2012b;Tsai et al, 2012;Ee et al, 2014;Liang et al, 2014 (Aggarwal et al, 2008;Estrela et al, 2008a;Jorge et al, 2008;Low et al, 2008).…”

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Tomografia computadorizada de feixe cônico como método de avaliação pós-tratamento endodôntico em sessão única ou em duas sessões

Maia¹

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, por sua dedicação à Endodontia, por sua contribuição à minha formação acadêmica e por ter realizado tanto pela nossa profissão.À Profa. Dra. Andiara De Rossi, por sua contribuição e observações na qualificação.À Profa. Dra. Raquel Assed Bezerra Segato, por sua contribuição na minha formação.Ao Prof. Dr. Idomeo Boneti Filho, por sua contribuição na minha formação acadêmica e profissional.À Profa. Dra. Angela Cristina Cilense Zuanon, por sua dedicação e motivação na minha formação profissional e pessoal.Ao Prof. Dr. Kleber Cortez Bonifácio, por sua amizade e incentivo para a realização do doutorado. AbstractIntroduction: Epidemiological studies have demonstrated a high prevalence of periapical lesion in different countries, stating that it is a health problem that affects great part of the world population. Aim: The aim of this randomized clinical study was to conduct a comparative evaluation after endodontic treatment of permanent teeth with chronic periapical lesion completed in a single session or in two sessions with 14-day calcium hydroxide interappointment intracanal medication, using cone beam computed tomography (CBCT). Methodology: Twenty-six pairs of teeth from 13 patients treated at the Endodontics Clinic of Ceuma University (UniCeuma), São Luís, MA, Brazil, were selected and randomly assigned to two groups (n=13/group) by simple random sampling (draw). In Group I, the teeth were instrumented and filled in the same session, while in Group II, after instrumentation, an intracanal medication (Calen ® paste) was used for 14 days between

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Cone-beam computerized tomographic, radiographic, and histologic evaluation of periapical repair in dogs' post-endodontic treatment

Abstract: CT Scan evaluation of periapical repair following root canal treatment provided similar information than that obtained by microscopic analysis, whereas radiographic evaluation underestimated the size do periapical lesion.

Cited by 87 publications

References 31 publications

Promotion of Endodontic Lesions in Rats by a Novel Extraradicular Biofilm Model Using Obturation Materials

Promotion of Endodontic Lesions in Rats by a Novel Extraradicular Biofilm Model Using Obturation Materials

Endodontic applications of cone beam computed tomography: case series and literature review

Tomografia computadorizada de feixe cônico como método de avaliação pós-tratamento endodôntico em sessão única ou em duas sessões

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