Conductive polymer-modified boron-doped diamond for DNA hybridization analysis

Recent advances in polyaniline based biosensors

“…DNA hybridization has been demonstrated onto ssDNA probe immobilized onto thin layer of PANI/PAA coated onto boron-doped diamond substrate using cyclic voltammetry (Gu et al, 2004). It has been reported that the ssDNA-modified PANI-intercalated graphite oxide nanocomposite onto carbon paste electrode can be used to monitor DNA hybridization with complementary ssDNA in the linear range of 275-551 g mL −1 and detection limit of 29.34 g mL −1 using square wave voltammetry (SWV) within 30 min (Wu et al, 2005).…”

Poly-l-lysine-modified boron-doped diamond electrodes for the amperometric detection of nucleic acid bases

“…Gu et al [45] demonstrated a one-step chemical modification of BDD by the thin polyaniline/poly (acrylic acid) (PANI/PAA) composite polymer film. The procedure did not show any non-specific DNA adsorption, and DNA probes immobilized on the BDD substrates were stable and selective to DNA sensing, while the oxidation peak potentials were lower than those reported for the oxidation of guanine and adenine on other carbon-based electrodes [22,23] and bare BDD [3,4].…”

Ultrasensitive DNA hybridization biosensor based on polyaniline

“…PANI nanowires have been fabricated onto Si substrates containing fully stretched DNA as growing templates using horseradish peroxidase enzyme (HRP) (Ma et al, 2004). DNA hybridization has been demonstrated onto single-stranded DNA probe immobilized onto thin layer of polyaniline/poly(acrylic acid) coated onto boron-doped diamond substrate using cyclic voltammetry (Gu et al, 2004). It has been reported that the single-stranded DNA-modified PANI-intercalated graphite oxide nanocomposite onto carbon paste electrode can be used to monitor DNA hybridization with complementary single-stranded DNA in the linear range of 275-551 g/ml and detection limit of 29.34 g/ml using square wave voltammetry within 30 min of hybridization time (Wu et al, 2005).…”