Conductive conduit based on electrospun poly (l-lactide-co-D, l-lactide) nanofibers containing 4-aminopyridine-loaded molecularly imprinted poly (methacrylic acid) nanoparticles used for peripheral nerve regeneration

Fallah-Darrehchi, Mahshid; Zahedi, Payam; Safarian, Shahrokh; Ghaffari-Bohlouli, Pejman; Aeinehvand, Robabeh

doi:10.1016/j.ijbiomac.2021.09.009

Cited by 12 publications

(7 citation statements)

References 33 publications

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“…In this study, the utilized LCE was based on a thermoresponsive mesogenic monomer in which T NI was more than the biological temperature, leading to the anisotropic mesomorph of samples in biological environments. As reported previously, PC12 cell line as neuroblasma cells is highly responsive to surface patterning and its performance in various developmental cell progressions can be simulated to peripheral nerve cells behavior. Optimized samples in terms of required properties, including LCE 3 film , LCE 3 fiber , neat PCL fiber , and PCL-LCE 3 fiber (2:1) nanofibers, were prepared.…”

Section: Resultsmentioning

confidence: 77%

Improvement of Intracellular Interactions through Liquid Crystalline Elastomer Scaffolds by the Alteration of Topology

Fallah-Darrehchi,

Zahedi

2023

ACS Omega

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Preparation of inherently bioactive scaffolds has become a challenging issue owing to their complicated synthesis and nonrobust modified cell-actuating property. Liquid crystalline elastomers (LCEs), due to their combined specialties of liquid crystals and elastomers as well as their ability to respond to various kinds of stimuli, have reversibly led to the design of a new class of stimuli-responsive tissue-engineered scaffolds. In this line, in the first stage of this research work, synthesis and evaluation of acrylate-based LCE films (LCE film ) encompassing mesogenic monomers are carried out. In the second step, the design of an affordable electrospinning technique for preparing LCE nanofibers (LCE fiber ) as a problematic topic, thanks to the low molecular weight of the mesogenic chains of LCEs, is investigated. For this purpose, two approaches are considered, including (1) photo-cross-linking of electrospun LCE fiber and (2) blending LCE with poly(ε-caprolactone) (PCL) to produce morphologically stable nanofibers (PCL-LCE fiber ). In the following, thermal, mechanical, and morphological evaluations show the optimized crosslinker (mol)/aliphatic spacer (mol) molar ratio of 50:50 for LCE film samples. On the other hand, for LCE fiber samples, the appropriate amounts of excessive mesogenic monomer and PCL/LCE (v/v) to fabricate the uniform nanofibers are determined to be 20% and 1:2, respectively. Eventually, PC12 cell compatibility and the impact of the liquid crystalline phase on the PC12 cell dynamic behavior of the samples are examined. The obtained results reveal that PC12 cells cultured on electrospun PCL-LCE fiber nanofibers with an average diameter of ∼659 nm per sample are alive and the scaffold has susceptibility for cell proliferation and actuation because of the rapid increase in cell density and number of singularity points formed in time-lapse cell imaging. Moreover, the PCL-LCE fiber nanofibrous scaffold exhibits a high performance for cell differentiation according to detailed biological evaluations such as gene expression level measurements. The time-lapse evaluation of PC12 cell flow fields confirms the significant influence of the reprogrammable liquid crystalline phase in the PCL-LCE fiber nanofibrous scaffold on topographical cue induction compared to the biodegradable PCL nanofibers.

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Section: Resultsmentioning

confidence: 77%

Improvement of Intracellular Interactions through Liquid Crystalline Elastomer Scaffolds by the Alteration of Topology

Fallah-Darrehchi,

Zahedi

2023

ACS Omega

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“…The degree of functional recovery of the injured nerve is assessed with the motor and sensory abilities of rats . To further evaluate the motor recovery, walking tracks were analyzed, and the sciatic function index (SFI) was calculated.…”

Section: Results and Discussionmentioning

confidence: 99%

“…The degree of functional recovery of the injured nerve is assessed with the motor and sensory abilities of rats. 45 To further evaluate the motor recovery, walking tracks were analyzed, and the sciatic function index (SFI) was calculated. Apparently, the footprint integrity of rats in S-SPH-0.3M and the autograft group is much higher than that of SPH-0.3M at 4 and 12 weeks (Figure 4b).…”

Section: Functional Recovery Of the Regenerated Sciatic Nervementioning

confidence: 99%

Electrospun Piezoelectric Scaffold with External Mechanical Stimulation for Promoting Regeneration of Peripheral Nerve Injury

Zhang,

Lan,

et al. 2023

Biomacromolecules

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Safe and efficient provision of electrical stimulation (ES) for nerve repair and regeneration is a problem that needs to be addressed. In this study, a silk fibroin/poly(vinylidene fluoride-co-hexafluoropropylene)/Ti3C2T x (SF/PVDF-HFP/MXene) composite scaffold with piezoelectricity was developed by electrospinning technology. MXene was loaded to the scaffold to enhance the piezoelectric properties (Output voltage reaches up to 100 mV), mechanical properties, and antibacterial activity. Cell experiments demonstrated piezoelectric stimulation under external ultrasonication for promoting the growth and proliferation of Schwann cells (SCs) cultured on this electrospun scaffold. Further in vivo study with rat sciatic nerve injury model revealed that the SF/PVDF-HFP/MXene nerve conduit could induce the proliferation of SCs, enhance the elongation of axon, and promote axonal myelination. Under the piezoelectric effect of this nerve scaffold, the rats with regenerative nerve exhibited a favorable recovery effect of motor and sensory function, indicating a safe and feasible method of using this SF/PVDF-HFP/MXene piezoelectric scaffold for ES provision in vivo.

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“…Encapsulated compounds are of interest to the food industry, as well as to the pharmaceutical industry, such as the polyphenols. Their antioxidant activities make them attractive to be encapsulated to maintain their bioactivity [ 53 , 54 ].…”

Section: Nanoparticles’ Preparation and Drug Loadingmentioning

confidence: 99%

“…In general, by minimizing the diameter of nanofibers, their specific surface area increases. Furthermore, the minimal size, together with a uniform morphology, of the non-beaded nanofibers leads to increased cell adhesion, improved drug loading, and controlled release [ 54 , 101 , 102 ].…”

Section: Materials Used In Hybrid Systemsmentioning

confidence: 99%

Hybrid Systems of Nanofibers and Polymeric Nanoparticles for Biological Application and Delivery Systems

et al. 2023

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Nanomedicine is a new discipline resulting from the combination of nanotechnology and biomedicine. Nanomedicine has contributed to the development of new and improved treatments, diagnoses, and therapies. In this field, nanoparticles have notable importance due to their unique properties and characteristics, which are useful in different applications, including tissue engineering, biomarkers, and drug delivery systems. Electrospinning is a versatile technique used to produce fibrous mats. The high surface area of the electrospun mats makes them suitable for applications in fields using nanoparticles. Electrospun mats are used for tissue engineering, wound dressing, water-treatment filters, biosensors, nanocomposites, medical implants, protective clothing materials, cosmetics, and drug delivery systems. The combination of nanoparticles with nanofibers creates hybrid systems that acquire properties that differ from their components’ characteristics. By utilizing nanoparticles and nanofibers composed of dissimilar polymers, the two synergize to improve the overall performance of electrospinning mats and nanoparticles. This review summarizes the hybrid systems of polymeric nanoparticles and polymeric nanofibers, critically analyzing how the combination improves the properties of the materials and contributes to the reduction of some disadvantages found in nanometric devices and systems.

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Conductive conduit based on electrospun poly (l-lactide-co-D, l-lactide) nanofibers containing 4-aminopyridine-loaded molecularly imprinted poly (methacrylic acid) nanoparticles used for peripheral nerve regeneration

Cited by 12 publications

References 33 publications

Improvement of Intracellular Interactions through Liquid Crystalline Elastomer Scaffolds by the Alteration of Topology

Improvement of Intracellular Interactions through Liquid Crystalline Elastomer Scaffolds by the Alteration of Topology

Electrospun Piezoelectric Scaffold with External Mechanical Stimulation for Promoting Regeneration of Peripheral Nerve Injury

Hybrid Systems of Nanofibers and Polymeric Nanoparticles for Biological Application and Delivery Systems

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