Conditions Affecting Sertoli Cells

Salameh, Wael A.; Swerdloff, Ronald S.

doi:10.1016/b978-012647751-1/50022-2

Cited by 4 publications

(2 citation statements)

References 297 publications

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“…Defects in these cells have been believed to contribute to abnormal spermatogenesis. 34 The possibility of beneficial hormonal effects of Leydig cell transplantation independent of their support of spermatogenesis also exits. Because Leydig cells are responsible for testosterone production, stem cell transplantations may replace the need of life-long testosterone supplementation in male hypogonadism or aging.…”

Section: The Gfp-positive Donor-derived Cells Colocalized With Cell-smentioning

confidence: 99%

Fate of Bone Marrow Stem Cells Transplanted into the Testis

Lue

Erkkilä

Liu

et al. 2007

The American Journal of Pathology

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105

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To assess adult stem cell differentiation in the testis, we injected bone marrow cells from adult green fluorescent protein (GFP) transgenic mice into the seminiferous tubules and the testicular interstitium of busulfan-treated wild-type or c-kit mutant (W/W v ) mice. Ten to 12 weeks after transplantation, we examined the fate of the transplanted bone marrow cells and found that they survived in recipient testes. In both the busulfan-treated and W/W v mice, some of the GFP-positive donor cells had a Sertoli cell appearance and expressed follicle-stimulating hormone receptor within the seminiferous tubules. In addition, GFP-positive donor cells were found in the interstitium of recipient testes, and they expressed the cytochrome P450 side chain cleavage enzyme (P450scc). In the seminiferous tubules of busulfan-treated mice, GFP-positive donor cells had the appearance of spermatogonia or spermatocytes and expressed VASA.

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Section: The Gfp-positive Donor-derived Cells Colocalized With Cell-smentioning

confidence: 99%

Fate of Bone Marrow Stem Cells Transplanted into the Testis

Lue

Erkkilä

Liu

et al. 2007

The American Journal of Pathology

Self Cite

105

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“…Because each SC can support only a fixed number of germ cells, the number of SCs is directly related to sperm counts. Thus, alterations in SC function could lead to impaired spermatogenesis, germ-cell loss and irreversible infertility (Salameh and Swerdloff 2005). Indeed, in our model of testicular HSV infection, SC death led to the atrophy of seminiferous epithelium and infertility; there were no signs of spermatogenic recovery even 100 days after infection .…”

Section: Introductionmentioning

confidence: 97%

Neonatal testicular cell transplantation restores murine spermatogenesis damaged in the course of herpes simplex virus-induced orchitis

Малолина

Kulibin

Кущ

2016

Reprod. Fertil. Dev.

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Genital tract infection and inflammation may affect male fertility, causing germ and Sertoli cell loss. We determined if testicular cell transplantation is effective at repairing testicular injury induced by herpes simplex virus (HSV) orchitis. ROSA26 mice were used as donors and the recipients were C57BL/6 mice after HSV testicular inoculation; some of the recipients were treated with the antiviral drug acyclovir (ACV). ACV reduced the amount of HSV antigen in testes on Day 3 after transplantation and enhanced the efficacy of transplantation at Day 30. In recipient testes, donor Sertoli cells formed new seminiferous tubules; significantly more new tubules were observed in the testes of ACV-treated mice compared with mice not treated with ACV (17.8% vs 3.6%). Over half (50.4%) of new tubules in ACV-treated testes contained germ cells and round spermatids were detected in 14.2% of new tubules compared with 15.9% and 5.3% in testes not treated with ACV, respectively. At Day 150 the seminiferous epithelium was completely recovered in some donor tubules and elongated spermatids were observed inside it. Thus, our findings reveal the effectiveness of the combination of antiviral therapy with neonatal testis-cell transplantation for the restoration of spermatogenesis damaged by viral infection.

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In vitro evaluation of cell viability and expression profile of growth factors in mouse Sertoli cells exposed to Delta-9-tetrahydrocannabinol: a mechanistic insight into the cannabinoid-induced testicular toxicity

Mohammadpour-Asl,

Roshan-Milani,

Abdollahzade Fard

et al. 2023

BMC Pharmacol Toxicol

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The potentially adverse effects of cannabis (marijuana), a common leisure compound, on male reproductive performance are a reason for concern. δ-9-tetrahydrocannabinol (THC), the primary active component of marijuana alters testicular cells’ proliferation and function which affects male fertility and causes testicular cells dysfunction and apoptosis. The main objective of this study was to investigate the possible mechanism underlying the toxic effects of THC with a mechanistic insight into Sertoli cell-based reproductive dysfunction. The Mus musculus Sertoli cell line (TM4) was cultured and exposed to different concentrations of THC and, MTT (3-(4, 5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was then performed for evaluating cell viability. The expression of caspase-3 gene and genes related to growth factors were analyzed by real-time RT-PCR. Western blotting was performed for evaluating protein expression level. THC concentration-dependently decreased the TM4 viability with a significant effect starting at concentration of 1 μM and reaching about 75% of the control level at the concentration of 50 μM (IC25). Moreover, caspase-3 mRNA expression levels significantly increased while growth factors mRNA levels decreased in THC-exposed cells compared to unexposed cells. There was also a significant reduction in related protein levels in THC group. Administration of the THC promotes cytotoxic and apoptotic effects on TM4 cells partly through down-regulation of growth factors expression. Increased apoptosis, over expression of caspase-3, and down-regulation of growth factors expression in Sertoli cells exposed to THC may be a reflection of THC-induced testicular toxicity, which may be partly involved in infertility associated with marijuana smoking or medical cannabis use.

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Conditions Affecting Sertoli Cells

Cited by 4 publications

References 297 publications

Fate of Bone Marrow Stem Cells Transplanted into the Testis

Fate of Bone Marrow Stem Cells Transplanted into the Testis

Neonatal testicular cell transplantation restores murine spermatogenesis damaged in the course of herpes simplex virus-induced orchitis

In vitro evaluation of cell viability and expression profile of growth factors in mouse Sertoli cells exposed to Delta-9-tetrahydrocannabinol: a mechanistic insight into the cannabinoid-induced testicular toxicity

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