Conditioning film formation and its influence on the initial adhesion and biofilm formation by a cyanobacterium on photobioreactor materials

Talluri, Suvarna; Winter, Robb M.; Salem, David R.

doi:10.1080/08927014.2020.1748186

Cited by 30 publications

(31 citation statements)

References 62 publications

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“…Considering the results observed in the short-term BSA adhesion tests, the above assumption is highly plausible. A recent study supports this point, in which proteins secreted by the cyanobacterium Annabaenasp were first adsorbed onto suitable PBR materials during the formation of the conditioning film (Talluri et al, 2020). Figure 4A demonstrates that protein adsorption on the coupon surface increased as protein concentration increased in the supernatant, as reported for BSA on surfaces immersed in seawater (Kirchman et al, 1989).…”

Section: Culture Experiments In the Vesselssupporting

confidence: 66%

“…These observations support the assumption that the initial event that initiates the biological response of an N. gaditana culture to artificial materials and coatings is the adsorption of proteins secreted by the microalgae into the culture medium. This is in accordance with results from studies on photobioreactor materials used in the cultivation of the cyanobacterium Anabaena sp., where proteins were present on the conditioning films formed on glass and PMMA (Talluri et al, 2020).…”

Section: Analysis In the Context Of The Baier And Vogler Biocompatibimentioning

confidence: 99%

“…A wide variety of anti-biofouling materials and coatings considered for use in PBRs has been studied (Talluri et al, 2020;Wang et al, 2017;Wang et al, 2020;Zeriouh et al, 2019a). All of them have failed to completely prevent long-term biofouling.…”

Section: Introductionmentioning

confidence: 99%

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Long-term biofouling formation mediated by extracellular proteins in Nannochloropsis gaditana microalga cultures at different medium N/P ratios

Soriano-Jerez¹,

López-Rosales²,

Cerón-García³

et al. 2020

Preprint

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Biofouling represents an important limitation in photobioreactor cultures. The biofouling propensity of different materials (polystyrene, borosilicate glass, polymethyl methacrylate and polyethylene terephthalate glycol-modified) and coatings (two spray-applied and nanoparticle-based superhydrophobic coatings and a hydrogel-based fouling release coating) was evaluated by means of a short-term protein test, using bovine serum albumin (BSA) as a model protein, and by the long-term culture of the marine microalga Nannochloropsis gaditana under practical conditions. The results from both methods were similar, confirming that the BSA test predicts microalgal biofouling on surfaces exposed to microalgae cultures; these secrete macromolecules, such as proteins, that have a high capacity for forming a conditioning film prior to cell adhesion. The hydrogel-based coating showed significantly reduced BSA and N. gaditana adhesion, whereas the other surfaces failed to control biofouling. Microalgal biofouling was associated with an increased concentration of sticky extracellular proteins at low N/P ratios (below 15).

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Section: Culture Experiments In the Vesselssupporting

confidence: 66%

Section: Analysis In the Context Of The Baier And Vogler Biocompatibimentioning

confidence: 99%

See 1 more Smart Citation

Long-term biofouling formation mediated by extracellular proteins in Nannochloropsis gaditana microalga cultures at different medium N/P ratios

Soriano-Jerez¹,

López-Rosales²,

Cerón-García³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…A recent study supports this point, in which proteins secreted by the cyanobacterium Annabaena sp. were first adsorbed onto suitable PBR materials during the formation of the conditioning film (Talluri et al, 2020). Figure 4a demonstrates that protein adsorption on the coupon surface increased as protein concentration increased in the supernatant, as reported for BSA on surfaces immersed in seawater (Kirchman et al, 1989).…”

Section: Resultsmentioning

confidence: 99%

“…A wide variety of anti‐biofouling materials and coatings considered for use in PBRs has been studied (Talluri et al, 2020; Wang et al, 2017, 2020; Zeriouh et al, 2019a). All of them have failed to completely prevent long‐term biofouling.…”

Section: Introductionmentioning

confidence: 99%

Long‐term biofouling formation mediated by extracellular proteins in Nannochloropsis gaditana microalga cultures at different medium N/P ratios

Soriano-Jerez

López-Rosales

Cerón-García

et al. 2020

Biotech & Bioengineering

View full text Add to dashboard Cite

Biofouling represents an important limitation in photobioreactor cultures. The biofouling propensity of different materials (polystyrene, borosilicate glass, polymethyl methacrylate, and polyethylene terephthalate glycol‐modified) and coatings (two spray‐applied and nanoparticle‐based superhydrophobic coatings and a hydrogel‐based fouling release coating) was evaluated by means of a short‐term protein test, using bovine serum albumin (BSA) as a model protein, and by the long‐term culture of the marine microalga Nannochloropsis gaditana under practical conditions. The results from both methods were similar, confirming that the BSA test predicts microalgal biofouling on surfaces exposed to microalgae cultures whose cells secrete macromolecules, such as proteins, with a high capacity for forming a conditioning film before cell adhesion. The hydrogel‐based coating showed significantly reduced BSA and N. gaditana adhesion, whereas the other surfaces failed to control biofouling. Microalgal biofouling was associated with an increased concentration of sticky extracellular proteins at low N/P ratios (below 15).

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Antimicrobial Studies of Black Silicon and Black Diamond Using Gram‐Positive Bacteria

Norouzi,

Woudstra,

Smith

et al. 2023

Adv Eng Mater

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Bactericidal surfaces are in high demand for biomedical as well as industrial applications. In this paper, we have investigated if black diamond is useful for this application. Black diamond is derived from black silicon, a silicon surface structured into nano‐sized needles. These needles can pierce through bacteria on contact. Black diamond is obtained by coating black silicon with a thin diamond film rendering the nanostructures more robust. We compared the bactericidal and anti‐bacterial properties of fluorine‐terminated and hydrogen‐terminated black diamonds with those for black silicon and for flat surfaces of diamond (on silicon) with the same terminations. In this study we evaluated the ability to repel and kill Gram‐positive S. aureus and S epidermidis, which have a thicker cell wall and are more mechanically robust than the bacteria that have been studied before. We evaluated the short‐term 1 h initial adhesion as well as long‐term 24 h biofilm formation. We found that the number of bacteria that initially adhered to the fluorine‐terminated black diamond surface was significantly reduced and had the highest dead bacterial ratio compared to fluorine‐terminated flat diamond, black silicon surfaces and hydrogen‐terminated diamond surfaces, respectively. Biofilm formation after 24 h showed that while all surfaces outperform glass over the long‐term (24 h), diamond‐coated surfaces with both fluorine and hydrogen termination have a significant inhibiting biofilm formation effect. In conclusion, fluorinated and hydrogenated diamond‐coated surfaces with and without nano‐needles have repelling, bactericidal and biofilm‐inhibiting effects on Gram‐positive bacterial strains and are promising antimicrobial surfaces.This article is protected by copyright. All rights reserved.

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Conditioning film formation and its influence on the initial adhesion and biofilm formation by a cyanobacterium on photobioreactor materials

Cited by 30 publications

References 62 publications

Long-term biofouling formation mediated by extracellular proteins in Nannochloropsis gaditana microalga cultures at different medium N/P ratios

Long-term biofouling formation mediated by extracellular proteins in Nannochloropsis gaditana microalga cultures at different medium N/P ratios

Long‐term biofouling formation mediated by extracellular proteins in Nannochloropsis gaditana microalga cultures at different medium N/P ratios

Antimicrobial Studies of Black Silicon and Black Diamond Using Gram‐Positive Bacteria

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