Conditioned Medium of Human Immortalized Mesenchymal Stem Cells as a Novel Therapeutic Tool to Repair Damaged Tissues

Ashiba, Keisuke; Terunuma, Atsushi; Deng, Xuewen; Watanabe, Kazuo

doi:10.1166/jnsne.2017.1107

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because of these properties, MSCs have attracted much interest in translational research as a therapeutic tool. MSCs, as well as stem cell-conditioned media and exosomes contained therein, have been used to treat diverse disorders [1] , including cerebral infarction [2] , spinal cord injury [3][4][5] , diabetes mellitus [6] , obesity [7], atopic dermatitis [8] , inflammatory bowel disease [9] and liver disorders [10] .…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptomic analysis of human mesenchymal stem cells derived from dental pulp and adipose tissues

Terunuma

Ashiba

Takane

et al. 2019

JSRM

View full text Add to dashboard Cite

Objective: Mesenchymal stem cells (MSCs) have been isolated from various human tissues. Although they share cardinal stem cell features of self-renewal and multi-potency, they also seem to possess distinct characteristics depending on the tissue types they originated from. When developing stem cell-based therapies, MSCs with the most desirable characteristics should be chosen. However, our knowledge on tissue typespecific characteristics of MSCs is limited. Here, we comparatively studied the gene expression profiles of MSCs from different tissue types, and predicted target diseases suitable for each type of MSCs. Methods: We harvested MSCs from human dental pulp and adipose tissue specimens and subjected them to gene expression microarray analysis. Characteristic gene expression signatures of the MSCs from each tissue type were identified using gene-annotation enrichment analysis.Results: Dental pulp-derived MSCs exhibited gene expression signatures of neuronal growth, while adipose tissue-derived MSCs exhibited signatures of angiogenesis and hair growth. MSCs from each tissue type expressed a discrete set of genes encoding secretory peptides, which may function as paracrine factors. Conclusions: MSCs derived from different tissue types demonstrated distinct gene expression signatures, which are suggestive of target diseases in clinical applications of the MSCs and stem cell-conditioned media. By expanding the analysis to MSCs from a wide range of tissue types, and by employing multiple omics approaches, a catalogue of MSCs and therapeutic targets can be generated. However, our understanding on such differences between MSCs from diverse tissues is limited. In this study, we compared human dental pulp-derived MSCs (DP-hMSCs) and adipose tissue-derived MSCs (AT-hMSCs) for their gene expression profiles to predict therapeutic targets suitable for each type of MSCs. Methods Cell sourcesExfoliated deciduous teeth (n=6), permanent tooth (n=1), and adipose tissue from abdomen (n=2), buccal pad (n=2) and orbit (n=2) were obtained from healthy donors with written informed consent. Tissues were enzymatically processed and cells were cultured using MesenCult-XF (STEMCELL Technologies, Vancouver, Canada). Fetal fibroblasts WI-38 were obtained from Cell Bank (JCRB Cell Bank, Osaka, Japan). Gene expression microarrayCultured cells were stored in RNAlater (Thermo Fisher Scientific, Tokyo, Japan) and total RNA was extracted using RNeasy Mini Kit (QIAGEN, Hilden, Germany). RNA samples were processed and applied to SurePrint G3 Human GE 8x60K v3 Microarray (Agilent, Santa Clara, USA) at Biomedical Center (Takara Bio, Yokka-ichi, Japan).

show abstract