Conditioned medium-electrospun fiber biomaterials for skin regeneration

Chen, Lu; Cheng, Liying; Wang, Zhen; Zhang, Jianming; Mao, Xiyuan; Liu, Zhimo; Zhang, Yuguang; Cui, Wenguo; Sun, Xiaoming

doi:10.1016/j.bioactmat.2020.08.022

Cited by 51 publications

(40 citation statements)

References 65 publications

(76 reference statements)

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“…The availability of tendon-inductive factors may have research and application impacts in both helping establish robust in vitro tendon differentiation protocols and facilitating the development of tendon tissue engineering approaches. The availability of bioactive CM with defined teno-inductive properties may be applied to either scaffold functionalisation (Burdette et al, 2018 ; Chen et al, 2021 ) or in nanoparticle generation (Felice et al, 2018 ), both of which may have a clinically relevant role in the development of a new therapies for tendon pathology (Daneshmandi et al, 2020 ; Rhatomy et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Amnion-Derived Teno-Inductive Secretomes: A Novel Approach to Foster Tendon Differentiation and Regeneration in an Ovine Model

Citeroni

Mauro

Ciardulli

et al. 2021

Front. Bioeng. Biotechnol.

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Regenerative medicine has greatly progressed, but tendon regeneration mechanisms and robust in vitro tendon differentiation protocols remain to be elucidated. Recently, tendon explant co-culture (CO) has been proposed as an in vitro model to recapitulate the microenvironment driving tendon development and regeneration. Here, we explored standardized protocols for production and storage of bioactive tendon-derived secretomes with an evaluation of their teno-inductive effects on ovine amniotic epithelial cells (AECs). Teno-inductive soluble factors were released in culture-conditioned media (CM) only in response to active communication between tendon explants and stem cells (CMCO). Unsuccessful tenogenic differentiation in AECs was noted when exposed to CM collected from tendon explants (CMFT) only, whereas CMCO upregulated SCXB, COL I and TNMD transcripts, in AECs, alongside stimulation of the development of mature 3D tendon-like structures enriched in TNMD and COL I extracellular matrix proteins. Furthermore, although the tenogenic effect on AECs was partially inhibited by freezing CMCO, this effect could be recovered by application of an in vivo-like physiological oxygen (2% O2) environment during AECs tenogenesis. Therefore, CMCO can be considered as a waste tissue product with the potential to be used for the development of regenerative bio-inspired devices to innovate tissue engineering application to tendon differentiation and healing.

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Section: Discussionmentioning

confidence: 99%

“…Moreover, lyophilisation improved the long-term stability of nanosized drug delivery, such as liposomes (Liu et al, 2015 ) and exosomes (Charoenviriyakul et al, 2018 ). It was also demonstrated as a solution to preserve the whole secretome (Fernandes-Cunha et al, 2019 ; Chen et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Amnion-Derived Teno-Inductive Secretomes: A Novel Approach to Foster Tendon Differentiation and Regeneration in an Ovine Model

Citeroni

Mauro

Ciardulli

et al. 2021

Front. Bioeng. Biotechnol.

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“…Also, the maintenance of skin integrity is a necessity for insuring the homeostasis of human body. However, major skin injuries from trauma or illness may dis-functionalize its primary defense capacity, resulting in serious infections or disabilities to endanger the quality of human life [ [4] , [5] , [6] ]. For instance, the failure in repair of chronic skin wounds such as diabetic foot ulcers may lead to amputation or other life-threatening consequences [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…Up to now, serious skin diseases remain a highly prevalent clinical problem with no satisfactory solution [ 8 ]. Therefore, it is meaning to develop biomaterials with high bioactivity for regeneration of skin diseases [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Incorporating redox-sensitive nanogels into bioabsorbable nanofibrous membrane to acquire ROS-balance capacity for skin regeneration

Zhang

Qiu

et al. 2021

Bioactive Materials

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Facing the high incidence of skin diseases, it is urgent to develop functional materials with high bioactivity for wound healing, where reactive oxygen species (ROS) play an important role in the wound healing process mainly via adjustment of immune response and neovasculation. In this study, we developed a kind of bioabsorbable materials with ROS-mediation capacity for skin disease therapy. Firstly, redox-sensitive poly(N-isopropylacrylamide-acrylic acid) (PNA) nanogels were synthesized by radical emulsion polymerization method using a disulfide molecule as crosslinker. The resulting nanogels were then incorporated into the nanofibrous membrane of poly( l -lactic acid) (PLLA) via airbrushing approach to offer bioabsorbable membrane with redox-sensitive ROS-balance capacity. In vitro biological evaluation indicated that the PNA-contained bioabsorbable membrane improved cell adhesion and proliferation compared to the native PLLA membrane. In vivo study using mouse wound skin model demonstrated that PNA-doped nanofibrous membranes could promote the wound healing process, where the disulfide bonds in them were able to adjust the ROS level in the wound skin for mediation of redox potential to achieve higher wound healing efficacy.

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“…It is important to consider that a particular design of a scaffold is appropriate for each specific type of wound. [53], [54]…”

Section: Recent Innovative Strategies In Wound Healing Regenerationmentioning

confidence: 99%

The Application of Mesenchymal Stem Cells on Wound Repair and Regeneration

Lopes¹,

Sousa²,

Alvites³

et al. 2021

Preprint

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In the past decades, regenerative medicine applied on skin lesions has been a field of constant improvement for both human and veterinary medicine. The process of healing cutaneous wound injuries implicates a well-organized cascade of molecular and biological processes. However, sometimes the normal process fails and can result in a chronic lesion. In addition, wounds are considered an increasing clinical impairment, due to the progressive ageing of the population, as well as the prevalence of concomitant diseases, such as diabetes and obesity, that represent risk aggravating factors for the development of chronic skin lesions. Stem cells regenerative potential has been recognized worldwide, including towards skin lesion repair, Tissue engineering techniques have long been successfully associated with stem cell therapies, namely the application of 3D bioprinted scaffolds. With this review we intend to explore several stem cell sources with promising aptitude towards skin regeneration, as well as different techniques used to deliver those cells and provide a supporting extracellular matrix environment, with effective outcomes. Furthermore, different studies are discussed, both in vitro and in vivo, towards their relevance in the skin regeneration field.

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Conditioned medium-electrospun fiber biomaterials for skin regeneration

Cited by 51 publications

References 65 publications

Amnion-Derived Teno-Inductive Secretomes: A Novel Approach to Foster Tendon Differentiation and Regeneration in an Ovine Model

Amnion-Derived Teno-Inductive Secretomes: A Novel Approach to Foster Tendon Differentiation and Regeneration in an Ovine Model

Incorporating redox-sensitive nanogels into bioabsorbable nanofibrous membrane to acquire ROS-balance capacity for skin regeneration

The Application of Mesenchymal Stem Cells on Wound Repair and Regeneration

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