Conditioned media derived from mesenchymal stem cells induces apoptosis and decreases cell viability and proliferation in squamous carcinoma cell lines

Bagheri, Rezvan; Bitazar, Razieh; Talebi, Saeed; Alaeddini, Mojgan; Etemad‐Moghadam, Shahroo; Eini, Leila

doi:10.1016/j.gene.2021.145542

Cited by 8 publications

(9 citation statements)

References 40 publications

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“…MSCs have been shown to have anti-tumor effects in in vitro and in vivo cancer models [40,41]. The therapeutic potential of MSCs is mediated by direct interaction with paracrine factors released from MSCs [42]. Recent studies have indicated that the anti-tumor properties of MSCs found in the different cancer cells are related to the various factors released by MSCs [43].…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Anti-Tumor Effect of Conditioned Media of Tonsil-Derived Mesenchymal Stem Cells on A549 and PANC-1 Cancer Cell Lines

Yuce

Albayrak

Gümüşkaptan

2022

Preprint

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Background Mesenchymal stem cells (MSCs) are the apple of the eye of cancer studies. It was indicated that the secreted factors, especially released by MSCs, have tumoral and anti-tumoral effects on tumor progression. MSCs obtained from different sources show different anti-tumoral effects. At the same time, MSCs originating from the same source also show different tumoral effects in different cancer cells. Here, we compared the anti-tumor effects of soluble factors secreted from MSCs derived from the palatine tonsil (T-MSC) as a new source of MSC on cancer cell lines A549 and PANC-1. Methods and Results Conditioned medium (CM) was obtained from T-MSCs that were successfully isolated from palatine tonsil tissue and characterized, and the cytotoxic effect of CM on the growth of A549 and PANC-1 at different concentrations was demonstrated by MTT analysis. In addition, the apoptotic effect of T-MSC-CM treatment was evaluated on cancer cells using Annexin-V/PI detection method by flow cytometry. Cell cycle arrest in cancer cells was analyzed by flow cytometry using propidium iodide (PI). As a result, we demonstrated that T-MSC-CM treatment significantly decreased the viability of A549 and PANC-1 cell lines in a dose dependent manner post 48 hours. Treatment of CM also differentially induced apoptosis on A549 and PANC-1 cells and caused G2/M arrest in cells. Conclusions In the light of these findings, our study is the first to show that T-MSC-CM has antitumor effect by stimulating apoptosis at different rates on A549 and PANC-1 cells, and has a cell-free cellular therapeutic potential.

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Section: Discussionmentioning

confidence: 99%

Comparison of the Anti-Tumor Effect of Conditioned Media of Tonsil-Derived Mesenchymal Stem Cells on A549 and PANC-1 Cancer Cell Lines

Yuce

Albayrak

Gümüşkaptan

2022

Preprint

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“…Subcutaneous injection of human BMSCs-microRNA-101-3p in nude mice revealed a significant reduction in tumor volume and weight, confirming the inhibitory effect of BMSCs derived microRNA-101-3p on tumor growth in vivo. Bagheri et al (2021) demonstrated that human BMSCs-CM exhibited a time-dependent inhibitory effect on TSCC cells CAL27, which showed the lowest cells viability after 72 h of co-culture with BMSCs-CM. The decrease of proliferation marker proliferating cell nuclear antigen, anti-apoptotic marker BCL-2 and Ki67 positive cells at 24 and 72 h of coculture indicated that BMSCs-CM decreased the proliferation of CAL27 cells and increased apoptosis.…”

Section: Inhibitory Effects Of Bmscs On Oral Cancermentioning

confidence: 97%

“… Bagheri et al (2021) demonstrated that human BMSCs-CM exhibited a time-dependent inhibitory effect on TSCC cells CAL27, which showed the lowest cells viability after 72 h of co-culture with BMSCs-CM. The decrease of proliferation marker proliferating cell nuclear antigen, anti-apoptotic marker BCL-2 and Ki67 positive cells at 24 and 72 h of co-culture indicated that BMSCs-CM decreased the proliferation of CAL27 cells and increased apoptosis.…”

Section: Effects Of Mesenchymal Stem Cells On Oral Cancermentioning

confidence: 97%

“…In a study of human amniotic membrane MSCs (HAMCSs)-derived CM, its promoting effect on TSCC cells CAL27 was demonstrated after a 24 h of co-culture with human HAMSCs-CM, manifested by an increase in CAL27 cell viability. After 72 h of co-culture, the increased expression of proliferating cell nuclear antigen indicated that HAMSCs-CM promoted the proliferation of CAL27, and the results of flow cytometry showed a decrease in the number of apoptotic CAL27 cells ( Bagheri et al, 2021 ).…”

Section: Effects Of Mesenchymal Stem Cells On Oral Cancermentioning

confidence: 99%

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The Effects of Mesenchymal Stem Cells on Oral Cancer and Possible Therapy Regime

et al. 2022

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Mesenchymal stem cells (MSCs) are characterized by self-renewal, rapid proliferation, multipotent differentiation, and low immunogenicity. In addition, the tropism of MSCs towards injured tissues and tumor lesions makes them attractive candidates as cell carriers for therapeutic agent delivery and genetic material transfer. The interaction between tumor cells and MSCs in the tumor microenvironment plays an important role in tumor progression. Oral cancer is one of the most common malignant diseases in the head and neck. Although considerable improvements in the treatment of oral cancer were achieved, more effective and safer novel agents and treatments are still needed, and deeper studies on the etiology, pathology, and treatment of the oral cancer are desirable. In the past decades, many studies have reported the beneficial effects of MSCs-based therapies in the treatment of various diseases, including oral cancers. Meanwhile, other studies demonstrated that MSCs may enhance the growth and metastasis of oral cancer. In this paper, we reviewed the research progress of the effects of MSCs on oral cancers, the underlying mechanisms, and their potential applications in the treatment of oral cancers.

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“…In a recent study, it has been demonstrated that colon cancer-derived CM repressed the monocyte proliferation and reduced the viability of THP-1 cells by arresting the cells at the G1-phase (Sawa-Wejksza et al, 2018). Another study revealed that squamous carcinoma cells viability and proliferation rate treated with mesenchymal stem cells CMs decreased significantly (Bagheri et al, 2021). Hence, CMs may have growth-limiting and anti-proliferative effects on target cells, as observed for SVG p12 cells treated with CMs obtained from LN229 cells.…”

Section: Cms Derived From U87 and Ln299 Cells Exhibited Diverse Effects On Svg P12 Viability And Clonogenicitymentioning

confidence: 98%

Condition Medium of Glioblastoma Cell Lines Decreases the Viability of Glioblastoma Cells by Modulating Gene Expression Profile

Yavuz

Akgül

Kaya

et al. 2021

Preprint

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Grade IV neoplasm of the central nervous system, GBM, is associated with poor prognosis and relatively short overall survival. Due to the current limitations in treatment methods, GBM is characterized as an incurable disease, and research to advance therapeutic options is required. Conditioned medium is commonly used in in-vitro studies complementary to animal experiments to simulate tumor microenvironment and has the potential to challenge and expand our current understanding of secretome effect on tumor characteristics. This study aimed to investigate the effects of conditioned mediums of GBM cell lines on each other. Conditioned mediums' cellular and molecular effects were evaluated using commonly employed techniques such as MTT assay, colony formation assay, wound healing assay, EdU labeling-based flow cytometry, and qRT-PCR. Our study demonstrated that conditioned medium harvested from U87 or LN229 cells at 48th h exhibited an anti-growth activity on each other by changing the gene expression pattern. Furthermore, the conditioned medium of LN229 decreased the migration capacity of U87 cells, and the conditioned medium of U87 cells significantly suppressed the LN229 proliferation. We believe that this initial work provides new insights for a better understanding of GBM cell lines' secretome roles and highlights the necessity of further studies to unveil the secretome content.

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Conditioned media derived from mesenchymal stem cells induces apoptosis and decreases cell viability and proliferation in squamous carcinoma cell lines

Cited by 8 publications

References 40 publications

Comparison of the Anti-Tumor Effect of Conditioned Media of Tonsil-Derived Mesenchymal Stem Cells on A549 and PANC-1 Cancer Cell Lines

Comparison of the Anti-Tumor Effect of Conditioned Media of Tonsil-Derived Mesenchymal Stem Cells on A549 and PANC-1 Cancer Cell Lines

The Effects of Mesenchymal Stem Cells on Oral Cancer and Possible Therapy Regime

Condition Medium of Glioblastoma Cell Lines Decreases the Viability of Glioblastoma Cells by Modulating Gene Expression Profile

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