Conditionally Redundant Bacteriocin Targeting by<i>Pseudomonas syringae</i>

Kl, Hockett; Clark, Meara; Scott, Samantha A.; Baltrus, David A.

doi:10.1101/167593

Cited by 18 publications

(22 citation statements)

References 75 publications

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“…This suggests that, in our strains, intact, correct length OSA is necessary for tailocin-mediated killing, likely as a receptor for tailocin binding. This concurs with, and expands on, recent work with Rp4 tailocins in P. syringae 21,37 .…”

Section: Genome-wide Mutant Fitness Assays Identify Gene Functions Insupporting

confidence: 90%

“…Phages are known to use both LPS moieties and membrane proteins as receptors 39 , but tailocins are only known to use LPS moieties as receptors 13,16,18,20,21,27,37,38 . Our data, which represents the most comprehensive genetic analysis so far of possible tailocin receptors, maintains this paradigm.…”

Section: Genome-wide Mutant Fitness Assays Identify Gene Functions Inmentioning

confidence: 99%

“…This was to perform quality filtering and trimming as well as remove Illumina PhiX174 spike-ins given in the file phix174_Illumina.fa. Assembly was performed using SPAdes version 3.13.0 73 with parameters -k 21,33,55,77,99,127.…”

Section: Genome Assemblymentioning

confidence: 99%

“…Despite their importance and potential, our knowledge of cellular elements with which tailocins interact remains limited, an issue exacerbated by the scarcity of genetic tools for undomesticated bacterial isolates. Biochemical [16][17][18] , and forward genetics studies 7,8,13,[19][20][21] in model strains suggest that tailocins bind to specific lipopolysaccharide (LPS) moieties, and that loss of these results in resistance. Nevertheless, it remains unclear whether LPS composition is the only determinant of tailocin sensitivity, and whether this differs between tailocin types.…”

Section: Introductionmentioning

confidence: 99%

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Systematic Discovery of Pseudomonad Genetic Factors Involved in Sensitivity to Tailocins

Carim

Azadeh

Kazakov

et al. 2020

Preprint

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Tailocins are bactericidal protein complexes produced by a wide variety of bacteria to compete against closely related strains. Like tailed bacteriophages, with whom they share an evolutionary and morphological relationship, tailocins bind and kill a narrow spectrum of target cells. Thanks to their high specificity, tailocins have garnered recent attention for their potential as precision antibacterial agents. Nevertheless, the field currently lacks a systematic investigation of genetic determinants of tailocin sensitivity. Here, we employed barcoded transposon-insertion mutant libraries and comparative genomics to assess genetic contributions to tailocin sensitivity in pseudomonads. Our mutant screens identified O-specific antigen (OSA) composition and display as most important in defining sensitivity to our tailocins. Additionally, the screens suggest lipopolysaccharide (LPS) thinning as a mechanism by which resistant strains can become more sensitive to tailocins. Our comparative genomics analyses show a loose relationship between OSA biosynthetic genes and tailocin sensitivity, as well as sensitivity nuances that require further investigation. Overall, our data reinforces the model that LPS molecules can act as either a receptor for, or shield against, tailocin binding and killing. This work offers insight into the specificity of tailocins and tailocin-mediated competition, informing the potential use of tailocins in microbiome manipulation and antibacterial therapy.

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Section: Genome-wide Mutant Fitness Assays Identify Gene Functions Insupporting

confidence: 90%

Section: Genome-wide Mutant Fitness Assays Identify Gene Functions Inmentioning

confidence: 99%

Section: Genome Assemblymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Systematic Discovery of Pseudomonad Genetic Factors Involved in Sensitivity to Tailocins

Carim

Azadeh

Kazakov

et al. 2020

Preprint

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“…Additionally, it was hypothesized that bacteria may be insensitive to phage infection in soil, because the phage receptor is not expressed in the metabolic state supported by the soil environment (59). Lipopolysaccharides (LPS) are commonly the receptor targeted by tailocin particles (17,18,21,22), and LPS biosynthesis is altered by growth phase and nutrient availability (60)(61)(62). In our study, a predominantly clay soil was used, and the extent to which this may have affected either tailocin activity or target sensitivity is unknown.…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas chlororaphis Produces Multiple R-Tailocin Particles That Broaden the Killing Spectrum and Contribute to Persistence in Rhizosphere Communities

Dorosky¹,

Pierson²,

Pierson

2018

Appl Environ Microbiol

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R-tailocins are high molecular weight bacteriocins resembling bacteriophage tails. 30-84 is a plant growth promoting rhizobacteria (PGPR) strain that produces two distinct R-tailocin particles with different killing spectra. The two R-tailocins have different evolutionary histories but are released by the same lysis cassette. A previous study showed that both tailocins are important for pairwise competition with susceptible rhizosphere colonizing strains, however, the broader role of tailocins in competition with the native rhizosphere microbiome was not tested. Genomic analysis of the 30-84 R-tailocin gene cluster uncovered the presence of three tail fiber genes in the tailocin 2 genetic module that could potentially result in tailocin 2 particles having different tail fibers and thus a wider killing spectrum. In this study, the tail fibers were found to incorporate onto different tailocin 2 particles, each with distinct killing spectra. Loss of production of one or both tailocins resulted in decreased 30-84 persistence within the wheat rhizosphere when in competition with the native microflora, but not bulk soil. The capacity to produce three different versions of a single tailocin, each having one of three different types of tail fibers is a previously unreported mechanism that leads to a broader R-tailocin killing spectrum. This study also provides evidence for the function of R-tailocins in competition with rhizosphere microbiome communities, but not in bulk soil. Although R-tailocin gene clusters typically encode one tail fiber protein, three tail fiber-resembling genes were identified in association with one of the two sets of R-tailocin genes within the tailocin cluster of 30-84 and other sequenced This study confirmed that 30-84 not only produces two distinct tailocins, but that one of them is produced with three different types of tail fibers. This is a previously unreported strategy to increase the breadth of strains targeted by an R-tailocin. Our finding that R-tailocins produced by a PGPR strain enhanced its persistence within the wheat rhizosphere microbiome confirms that R-tailocin production contributes to the population dynamics of rhizobacterial communities.

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Challenges of using protein antibiotics for pathogen control

Chai

Rooney

Milner

et al. 2021

Pest Management Science

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Bacterial phytopathogens represent a significant threat to many economically important crops. Current control measures often inflict harm on the environment and may ultimately impact on human health through the spread of antibiotic resistance. Antimicrobial proteins such as bacteriocins have been suggested as the next generation of disease control agents since they are able to specifically target the pathogen of interest with minimal impact on the wider microbial community and environment. However, substantial gaps in knowledge with regards to the efficacy and application of bacteriocins to combat phytopathogenic bacteria remain. Here we highlight the immediate challenges the community must address to ensure maximum exploitation of antimicrobial proteins in the field. © 2021 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Conditionally Redundant Bacteriocin Targeting byPseudomonas syringae

Abstract: 24The widespread use of antimicrobials under clinical and agricultural settings has 25 resulted in the evolution of resistance to these compounds.

Cited by 18 publications

References 75 publications

Systematic Discovery of Pseudomonad Genetic Factors Involved in Sensitivity to Tailocins

Systematic Discovery of Pseudomonad Genetic Factors Involved in Sensitivity to Tailocins

Pseudomonas chlororaphis Produces Multiple R-Tailocin Particles That Broaden the Killing Spectrum and Contribute to Persistence in Rhizosphere Communities

Challenges of using protein antibiotics for pathogen control

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