Conditional Reprogramming Modeling of Bladder Cancer for Clinical Translation

Daneshdoust, Danyal; Yin, Ming; Luo, Min; Sundi, Debasish; Dang, Yongjun; Lee, Cheryl; Li, Jenny; Liu, Xuefeng

doi:10.3390/cells12131714

Cited by 3 publications

(4 citation statements)

References 95 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CR’s induction of stem cell properties enhances cellular diversity representation within tumors, aiding in dissecting complex cell population interplay in cancer [ 28 ]. This versatility augments cell culture models, providing a dynamic platform to explore cellular behaviors in normal and cancerous tissues.…”

Section: Role Of Cr In Cell Culturementioning

confidence: 99%

Patient-Derived Conditionally Reprogrammed Cells in Prostate Cancer Research

Elbialy,

Kappala,

Desai

et al. 2024

Cells

Self Cite

View full text Add to dashboard Cite

Prostate cancer (PCa) remains a leading cause of mortality among American men, with metastatic and recurrent disease posing significant therapeutic challenges due to a limited comprehension of the underlying biological processes governing disease initiation, dormancy, and progression. The conventional use of PCa cell lines has proven inadequate in elucidating the intricate molecular mechanisms driving PCa carcinogenesis, hindering the development of effective treatments. To address this gap, patient-derived primary cell cultures have been developed and play a pivotal role in unraveling the pathophysiological intricacies unique to PCa in each individual, offering valuable insights for translational research. This review explores the applications of the conditional reprogramming (CR) cell culture approach, showcasing its capability to rapidly and effectively cultivate patient-derived normal and tumor cells. The CR strategy facilitates the acquisition of stem cell properties by primary cells, precisely recapitulating the human pathophysiology of PCa. This nuanced understanding enables the identification of novel therapeutics. Specifically, our discussion encompasses the utility of CR cells in elucidating PCa initiation and progression, unraveling the molecular pathogenesis of metastatic PCa, addressing health disparities, and advancing personalized medicine. Coupled with the tumor organoid approach and patient-derived xenografts (PDXs), CR cells present a promising avenue for comprehending cancer biology, exploring new treatment modalities, and advancing precision medicine in the context of PCa. These approaches have been used for two NCI initiatives (PDMR: patient-derived model repositories; HCMI: human cancer models initiatives).

show abstract

Section: Role Of Cr In Cell Culturementioning

confidence: 99%

Patient-Derived Conditionally Reprogrammed Cells in Prostate Cancer Research

Elbialy,

Kappala,

Desai

et al. 2024

Cells

Self Cite

View full text Add to dashboard Cite

show abstract

“…These properties qualified CR as an outstanding in vitro model compared to other models such as organoids and patient-derived xenografts [64]. Clinical research applications of CR technology have been investigated in breast cancer [75], lung cancer [76], prostate cancer [71], bladder cancer [77], gastric cancer [78], liver cancer [79], and salivary cancer (68).…”

Section: Conditionally Reprogrammed Cellsmentioning

confidence: 99%

“…It can be applied to many kinds of mammals such as mice, rats, horses, dogs, and cows [81]. Recent publications summarized applications of CR in cell biology, virology, and cancer research [24,64,66,77,78,[82][83][84][85]. In this article, we review studies that applied CR technology for breast cancer research (Figure 1).…”

Section: Conditionally Reprogrammed Cellsmentioning

confidence: 99%

“…Genetic analysis has recently revolutionized how different malignancies are classified and treated. Precision medicine is built on targeted therapy, a therapeutic approach that uses drugs to specifically target proteins and genes linked to the growth and survival of tumor cells [77]. Due to the lack of suitable in vitro models for breast cancer, this is a major concern in studying drug resistance and treatment response.…”

Section: Precision Medicine and Drug Discoverymentioning

confidence: 99%

See 1 more Smart Citation

Unlocking Translational Potential: Conditionally Reprogrammed Cells in Advancing Breast Cancer Research

Daneshdoust,

Luo,

et al. 2023

Cells

Self Cite

View full text Add to dashboard Cite

Preclinical in vitro models play an important role in studying cancer cell biology and facilitating translational research, especially in the identification of drug targets and drug discovery studies. This is particularly relevant in breast cancer, where the global burden of disease is quite high based on prevalence and a relatively high rate of lethality. Predictive tools to select patients who will be responsive to invasive or morbid therapies (radiotherapy, chemotherapy, immunotherapy, and/or surgery) are relatively lacking. To be clinically relevant, a model must accurately replicate the biology and cellular heterogeneity of the primary tumor. Addressing these requirements and overcoming the limitations of most existing cancer cell lines, which are typically derived from a single clone, we have recently developed conditional reprogramming (CR) technology. The CR technology refers to a co-culture system of primary human normal or tumor cells with irradiated murine fibroblasts in the presence of a Rho-associated kinase inhibitor to allow the primary cells to acquire stem cell properties and the ability to proliferate indefinitely in vitro without any exogenous gene or viral transfection. This innovative approach fulfills many of these needs and offers an alternative that surpasses the deficiencies associated with traditional cancer cell lines. These CR cells (CRCs) can be reprogrammed to maintain a highly proliferative state and reproduce the genomic and histological characteristics of the parental tissue. Therefore, CR technology may be a clinically relevant model to test and predict drug sensitivity, conduct gene profile analysis and xenograft research, and undertake personalized medicine. This review discusses studies that have applied CR technology to conduct breast cancer research.

show abstract