Conditional deletion of ELL2 induces murine prostate intraepithelial neoplasia

Pascal, Laura E.; Masoodi, Khalid Z.; Liu, June; Qiu, Xiaonan; Song, Qiong; Wang, Yujuan; Zang, Yu‐Feng; Yang, Tiejun; Wang, Yao; Rigatti, Lora H.; Chandran, Uma; Colli, Leandro M.; Vêncio, Ricardo Zorzetto Nicoliello; Lü, Yi; Zhang, Jian; Wang, Zhou

doi:10.1530/joe-17-0112

Cited by 12 publications

(37 citation statements)

References 53 publications

(72 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies suggested that ELL2 expression is regulated by androgens in prostate cancer cells . We confirmed ELL2 induction by androgens in two different prostate cancer cell lines LNCaP and C4‐2.…”

Section: Resultssupporting

confidence: 85%

“…Of these genes, 49 had a statistically significant tendency to co‐occur in tumor specimens with ELL2 alteration, while two were mutually exclusive (Table ). None of the identified AR response genes were identified previously as also being regulated by ELL2 or EAF2 . Interestingly, only SPDEF and TM4SF1 were identified as having a statistically significant tendency to co‐occur with EAF2 alteration in prostate cancer specimens (data not shown).…”

Section: Resultsmentioning

confidence: 86%

“…ELL2 immunostaining intensity was reduced in prostate cancer specimens, with more dramatic downregulation in high‐Gleason score prostate cancer specimens. ELL2 immunostaining were observed in all the low‐Gleason score prostate cancer specimens. However, there was a shift toward reduced ELL2 staining in low‐Gleason prostate specimens as compared to the benign prostate control ( P = 0.0004).…”

Section: Resultsmentioning

confidence: 93%

“…Our previous studies showed that the ELL2 mRNA was downregulated in prostate cancer specimens, particularly in high Gleason grade prostate cancer specimens . ELL2 mutation is not frequent in prostate cancer . However, the protein level of ELL2 was not evaluated previously in human prostate specimens.…”

Section: Resultsmentioning

confidence: 97%

“…ELL2 mRNA expression was downregulated in high Gleason grade prostate cancer specimens . Knockdown of ELL2 by siRNA resulted in increased proliferation, migration, and invasion in prostate cancer cell lines . Furthermore, prostate‐specific ELL2 knockout resulted in high‐grade murine prostatic intraepithelial neoplasia in the mouse model .…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Regulation of ELL2 stability and polyubiquitination by EAF2 in prostate cancer cells

Yang

Jing²,

Dong³

et al. 2018

The Prostate

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Resultssupporting

confidence: 85%

Section: Resultsmentioning

confidence: 86%

Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Regulation of ELL2 stability and polyubiquitination by EAF2 in prostate cancer cells

Yang

Jing²,

Dong³

et al. 2018

The Prostate

Self Cite

View full text Add to dashboard Cite

show abstract

Association of High miR-182 Levels with Low-Risk Prostate Cancer

Baumann

Acosta

Richards

et al. 2019

The American Journal of Pathology

View full text Add to dashboard Cite

A subset of men with prostate cancer develops aggressive disease. We sought to determine whether miR-182, an miRNA with reported oncogenic functions in the prostate, is associated with biochemical recurrence and aggressive disease. Prostate epithelial miR-182 expression was quantified via in situ hybridization of two prostate tissue microarrays and by laser-capture microdissection of prostate epithelium. miR-182 was significantly higher in cancer epithelium than adjacent benign epithelium ( P < 0.0001). The ratio of cancer to benign miR-182 expression per patient was inversely associated with recurrence in a multivariate logistic regression model (odds ratio = 0.18; 95% CI, 0.03–0.89; P = 0.044). Correlation of miR-182 with mRNA expression in laser-capture microdissected benign prostate epithelium was used to predict prostatic miR-182 targets. Genes that were negatively correlated with miR-182 were enriched for its predicted targets and for genes previously identified as up-regulated in prostate cancer metastases. miR-182 expression was also negatively correlated with genes previously identified as up-regulated in primary prostate tumors from African American patients, who are at an increased risk of developing aggressive prostate cancer. Taken together, these results suggest that although miR-182 is expressed at higher levels in localized prostate cancer, its levels are lower in aggressive cancers, suggesting a biphasic role for this miRNA that may be exploited for prognostic and/or therapeutic purposes to reduce prostate cancer progression.

show abstract

ELL2 regulates DNA non-homologous end joining (NHEJ) repair in prostate cancer cells

et al. 2018

Self Cite

View full text Add to dashboard Cite

ELL2 is an androgen-responsive gene that is expressed by prostate epithelial cells and is frequently down-regulated in prostate cancer. Deletion of Ell2 in the murine prostate induced murine prostatic intraepithelial neoplasia and ELL2 knockdown enhanced proliferation and migration in C4-2 prostate cancer cells. Here, knockdown of ELL2 sensitized prostate cancer cells to DNA damage and overexpression of ELL2 protected prostate cancer cells from DNA damage. Knockdown of ELL2 impaired non-homologous end joining repair but not homologous recombination repair. Transfected ELL2 co-immunoprecipitated with both Ku70 and Ku80 proteins. ELL2 could bind to and co-accumulate with Ku70/Ku80 proteins at sites of DNA damage. Knockdown of ELL2 dramatically inhibited Ku70 and Ku80 recruitment and retention at DNA double-strand break sites in prostate cancer cells. The impaired recruitment of Ku70 and Ku80 proteins to DNA damage sites upon ELL2 knockdown was rescued by re-expression of an ELL2 transgene insensitive to siELL2. This study suggests that ELL2 is required for efficient NHEJ repair via Ku70/Ku80 in prostate cancer cells.

show abstract

Conditional deletion of ELL2 induces murine prostate intraepithelial neoplasia

Cited by 12 publications

References 53 publications

Regulation of ELL2 stability and polyubiquitination by EAF2 in prostate cancer cells

Regulation of ELL2 stability and polyubiquitination by EAF2 in prostate cancer cells

Association of High miR-182 Levels with Low-Risk Prostate Cancer

ELL2 regulates DNA non-homologous end joining (NHEJ) repair in prostate cancer cells

Contact Info

Product

Resources

About