Condition Parameters of White-Tailed Deer in Texas

Kie, John G.; White, Marshall; Drawe, D. Lynn

doi:10.2307/3808596

Cited by 59 publications

(45 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The total protein and albumin levels of all serum samples used were determined a ; average values for the sera were within normal ranges for each species (using overall mean values for males and females of all age groups). 4,17,27,31 The positive control was Ig from 7 bovine sera (bovine Ig has previously been proven capable of binding to protein G). 20 The negative control was Ig from 8 chicken sera because Ig of avian species do not bind protein G. 3 Commercially purified bovine IgG b (92% purity via fractionation and ion-exchange chromatography) was included for a qualitative binding comparison.…”

Section: Samplesmentioning

confidence: 99%

Protein G Binding to Enriched Serum Immunoglobulin from Nondomestic Hoofstock Species

2003

View full text Add to dashboard Cite

Abstract. Quick and cost-effective serologic assays, such as those based on enzyme-linked immunosorbent assay (ELISA) technology, are useful for screening animal populations for infectious diseases. Recombinant protein G is described as an almost universal ELISA conjugate for the detection of antibodies from a wide range of animal species. However, there is limited data documenting the ability of protein G to bind immunoglobulin (Ig) from many captive and free-ranging nondomestic hoofstock (Order Artiodactyla, e.g., elk, antelope, bison). Protein G binding to Ig from 11 species within this taxonomic order (addax, antelope, bison, bontebok, elk, impala, kudu/nyala, muntjac, oryx, sheep, and white-tailed deer) and 2 control species (bovine and chicken) was assessed. A serum Ig enrichment protocol, using high-performance liquid chromatography (HPLC), was optimized in bovids (Bos taurus) and then applied to the other study species. Binding assays were performed by adding protein G to microtiter wells coated with titrated dilutions of enriched artiodactyl Ig. Optical densities were measured and binding curves generated. Differences in protein G binding were observed, both within and among species, as well as within taxonomic families. Significant intraspecies binding variation was observed for 7 species tested (antelope, oryx, sheep, muntjac, impala, bontebok, and addax). No statistically significant intraspecies differences in protein G binding were found for Ig from bison, elk, kudu/nyala, whitetailed deer, plus control species (cattle and chicken). Binding of protein G to Ig from impala, muntjac, and elk was statistically different from the positive control (cattle), with muntjac binding curves statistically comparable with the negative control (chicken). For the other 7 species tested, binding curves illustrated the ability of protein G to bind Ig as well as, or better than, the positive control. These findings expand the list of animal species whose Ig is capable of being detected using recombinant protein G, with the caveat that protein G does not bind Ig uniformly in closely related species. It is concluded that recombinant protein G conjugates may serve as useful reagents for serodiagnosis by ELISA in nondomestic hoofstock, although different assay interpretation algorithms and assay protocols may need to be developed on a per species basis for maximum diagnostic effectiveness.Detection, control, and management of infectious diseases in captive and free-ranging nondomestic ruminant hoofstock species (Order Artiodactyla) often prove challenging. These species, such as okapi, impala, and bontebok, are not often handled, and as a result, samples for diagnostic analysis are infrequently collected. An additional hindrance to disease surveillance is the lack of validated diagnostic assays for these valuable, and in some cases, endangered species.Reliable, cost-effective, and noninvasive diagnostics are needed to maximize the value of diagnostic samples when they can be collected. In particular, for diseases eliciti...

show abstract

Section: Samplesmentioning

confidence: 99%

Protein G Binding to Enriched Serum Immunoglobulin from Nondomestic Hoofstock Species

2003

View full text Add to dashboard Cite

show abstract

“…DeLiberto et al (1989) reported that K + values were highest in the summer, while several studies have shown no seasonal variation in K + (Seal et al, 1978;Kie et al, 1983;Waid and Warren, 1984;Jopson et al, 1997). DelGiudice et al (1992) investigated seasonal effects on blood parameters of white-tailed deer and reported the most probable cause of hyperkalemia during winter was a partial renal shutdown associated with reduced circulatory volume.…”

Section: Discussionmentioning

confidence: 99%

“…Many reference ranges have been reported for potassium (K + ) in freeranging cervids (Table 1; Wilber and Robinson, 1958;White and Cook, 1974;Seal et al, 1978;Kie et al, 1983;Waid and Warren, 1984;DeLiberto et al, 1989;DelGiudice et al, 1992). The International Species Information System (ISIS), widely used as a reference for captive populations, reports a mean K + of 4.360.9 milliequivalents (mEq)/l (n561; ISIS, 2002) for white-tailed deer (Odocoileus virginianus).…”

Section: Introductionmentioning

confidence: 99%

Hyperkalemia in Free-Ranging White-Tailed Deer (Odocoileus Virginianus)

Stringer

Kennedy‐Stoskopf

Chitwood

et al. 2011

Journal of Wildlife Diseases

View full text Add to dashboard Cite

ABSTRACT:Sixty adult and yearling female white-tailed deer (Odocoileus virginianus) were collected in July 2008 (n530) and March 2009 (n530) from eastern North Carolina as part of a population health assessment. During July 2008, standard serum analyses revealed hyperkalemia in all deer sampled. In March, the effect of processing time as a possible source of the hyperkalemia was investigated. For a subset of deer (n510), blood tubes were centrifuged and processed at four time points (0, 30, 60, and 120 min) postcollection. Delayed centrifugation and plasma separation did not affect potassium (K + ) concentration over time, indicating that a shift in intracellular K + did not occur and the hyperkalemia was not due to improper sample handling. Potassium levels were negatively correlated with age and varied across collection periods. Also, K + levels were positively correlated with glucose and not correlated with creatine kinase (CK). No single variable indicated a strong enough relationship to explain the hyperkalemia in the study.

show abstract

“…Physiological characteristics of white-tailed deer Odocoileus virginianus (Zimmermann, 1780) populations have been instrumental in assessing relative health of populations (Hesselton and Sauer 1973, Franzmann and LeResche 1978, Seal et al 1978, Kie et al 1983, Swihart et al 1998. Density, winter nutrition, and competitive interactions can negatively affect deer condition and reproduction and predispose deer to disease.…”

Section: Introductionmentioning

confidence: 99%

Effect of domestic cattle on the condition of female white-tailed deer in southern pine-bluestem forests, USA

Jenks

Leslie

2003

Acta Theriol

View full text Add to dashboard Cite

2003. Effect of domestic cattle on the condition of female white-tailed deer in southern pine-bluestem forests, USA. Acta Theriologica 48: 131-144.Effect of domestic cattle stocking on the nutritional condition of white-tailed deer Odocoileus virginianus (Zimmermann, 1780) was assessed using physiological indices of collected specimens. Three study areas were delineated in McCurtain County, Oklahoma (heavy cattle stocking), and Howard (moderate to light cattle stocking) and Pike (no cattle stocking) counties, Arkansas that were similar with respect to soils and vegetation but differed with respect to cattle stocking rate. Female white-tailed deer were collected from study areas in February and August 1987-1988 to assess nutritional condition. Deer collected from study areas exposed to cattle grazing in February had lower carcass weights, fat attributes (femur marrow and kidney fat), and reproductive rates (fetuses/doe) than deer that were not exposed to cattle grazing. In August, deer collected from the moderate cattle area had heavier eviscerated carcass weights, serum glucose, albumin, and albumin/globulin ratios than deer collected from the heavy cattle area. Results suggest that if cattle are removed from managed forests in winter, nutritional condition of deer would be improved because of reduced competition for food.

show abstract

Condition Parameters of White-Tailed Deer in Texas

Cited by 59 publications

References 17 publications

Protein G Binding to Enriched Serum Immunoglobulin from Nondomestic Hoofstock Species

Protein G Binding to Enriched Serum Immunoglobulin from Nondomestic Hoofstock Species

Hyperkalemia in Free-Ranging White-Tailed Deer (Odocoileus Virginianus)

Effect of domestic cattle on the condition of female white-tailed deer in southern pine-bluestem forests, USA

Contact Info

Product

Resources

About