CONDITION OPTIMIZATION PRODUCTION XYLANASE THERMOSTABLE BY Bacillus licheniformis TS10 USING SUBSTRATE OIL PALM EMPTY FRUIT BUNCHES

Abstract

ABSTRAKOptimasi produksi xilanase termostabil oleh Bacillus licheniformis TS10 menggunakan substrat tandan kosong kelapa sawit telah dilakukan di Laboratorium Genetika dan Bioteknologi, Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Sriwijaya, Indralaya. Penelitian dilakukan sejak November 2015 hingga Januari 2016. Penelitian ini bertujuan untuk mengetahui potensi tandan kosong kelapa sawit (TKKS) sebagai substrat untuk produksi xilanase termostabil oleh Bacillus licheniformis TS10 serta kondisi optimum suhu, pH dan konsentrasi substrat pada proses fermentasi. Metode penelitian adalah dengan membuat kurva pertumbuhan dan kurva produksi enzim dari Bacillus licheniformis TS10 pada substrat TKKS. Jumlah sel bakteri ditentukan dengan menggunakan spektrophotometer UV-VIS dan total plate count (TPC) melalui kurva standar. Optimasi dilakukan pada variasi pH (5, 6, 7, 8, 9), suhu (50°C, 60 °C, 70 °C, 80 °C) dan substrat (1%, 2%, 3%, 4%). Uji aktivitas pada masingmasing pH, suhu dan substrat menggunakan metode DNS dengan mengukur aktivitas enzim berdasarkan hasil gula reduksi yang dilepaskan oleh substrat dengan menggunakan dinitrosalicylic acid (DNS). Berdasarkan hasil penelitian dapat diketahui bahwa tandan kosong kelapa sawit (TKKS) memiliki potensi sebagai substrat untuk produksi xilanase termostabil oleh Bacillus licheniformis TS10, produksi xilanase termostabil dari Bacillus licheniformis TS10 pada substrat TKKS memiliki kondisi optimum pada pH 6, suhu 80 °C dan konsentrasi substrat sebesar 4%.Kata kunci : Optimasi, Xilanase Termostabil, Bacillus licheniformis TS10, Tandan Kosong Kelapa Sawit. ABSTRACTOptimization of thermostable xylanase production by Bacillus licheniformis TS10 using substrate oil palm empty fruit bunches has been conducted from November 2015 to January 2016 in the Laboratory of Genetics and Biotechnology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Indralaya. The aims to determine the potential of oil palm empty fruit bunches (EFB) as a substrate for the production of thermostable xylanase by Bacillus licheniformis TS10 and the optimum conditions of temperature, pH and substrate concentration in the fermentation process. The research method are to make the growth curve and the curve of Bacillus licheniformis TS10 enzyme production on EFB substrate. The number of bacterial cells was determined using a UV-VIS spectrophotometer and total plate count (TPC) through a standard curve. The optimization performed of pH (5, 6, 7, 8, 9), temperature (50 °C, 60 °C, 70 °C, 80 °C) and substrate (1%, 2%, 3%, 4%). The test activity in various pH, temperature and substrate using methods DNS by measuring enzyme activity based on the reducing sugar released by the substrate by using Dinitro Salicylic Acid (DNS). The results showed that oil palm empty fruit bunches (EFB) has a potential as a substrate for the production of thermostable xylanase by Bacillus licheniformis TS10, thermostable xylanase production of Bacillus licheniformis TS1...

Short Communication: The emergence and rise of indigenous thermophilic bacteria exploration from hot springs in Indonesia

“…In addition, it is important to evaluate enzyme production optimization strategies both on a lab and large scale (industry). Surprisingly, there is small number of publications in this aspect (Wibowo et al 2016;Indriati and Megahati 2017;Safitri et al 2017). However, the enzymes observed in Indonesia are imported, although industrial scale research is required to generate more indigenous applications to be produced.…”