Condensin I Interacts with the PARP-1-XRCC1 Complex and Functions in DNA Single-Strand Break Repair

Abstract

Condensins are essential protein complexes critical for mitotic chromosome organization. Little is known about the function of condensins during interphase, particularly in mammalian cells. Here we report the interphase-specific interaction between condensin I and the DNA nick-sensor poly(ADP-ribose) polymerase 1 (PARP-1). We show that the association between condensin I, PARP-1, and the base excision repair (BER) factor XRCC1 increases dramatically upon single-strand break damage (SSB) induction. Damage-specific association of condensin I with the BER factors flap endonuclease 1 (FEN-1) and DNA polymerase delta/epsilon was also observed, suggesting that condensin I is recruited to interact with BER factors at damage sites. Consistent with this, DNA damage rapidly stimulates the chromatin association of PARP-1, condensin I, and XRCC1. Furthermore, depletion of condensin in vivo compromises SSB but not double-strand break (DSB) repair. Our results identify a SSB-specific response of condensin I through PARP-1 and demonstrate a role for condensin in SSB repair.

Chromatin maintenance by a molecular motor protein

“…Given its role in interphase chromosome structure, however, it is possible that the aberrant interphase chromosome structure and replication defects also contribute to premature chromosome condensation, genomic instability and tumor formation. Interestingly, KIF4, PARP1 and condensin have all been shown to interact with each other 38 and interactions between condensin, KIF4 and PARP-1 10,18,38 may act in concert in chromosome structure maintenance and genome integrity. 9 Importantly, PARP-1 also interacts with condensins 38 and has recently been implicated in homologous recombination on damaged DNA.…”

“…Interestingly, KIF4, PARP1 and condensin have all been shown to interact with each other 38 and interactions between condensin, KIF4 and PARP-1 10,18,38 may act in concert in chromosome structure maintenance and genome integrity. 9 Importantly, PARP-1 also interacts with condensins 38 and has recently been implicated in homologous recombination on damaged DNA. 9,39 Furthermore, the interaction between condensin and PARP-1 is particularly strong during S phase.…”

Condensin II Alleviates DNA Damage and Is Essential for Tolerance of Boron Overload Stress in Arabidopsis

“…Animal condensins are localized to chromosomes during mitosis and are required for proper chromosomal condensation and segregation (Ono et al, 2003;Hirota et al, 2004;Ono et al, 2004). Additionally, it has been suggested that condensins have roles in the repair of DNA double-strand breaks (DSBs) and single-stranded DNA (ssDNA) (Heale et al, 2006;Wood et al, 2008).…”

Escherichia coli condensin MukB stimulates topoisomerase IV activity by a direct physical interaction

“…In eukaryotes, it is well established that the SMC complexes interact transiently with binding partners in addition to their stably associated, non-SMC accessory subunits, including the condensin partners Cti1 and PARP-1-XRCC1 and the cohesin partner Wap I (32)(33)(34). In contrast, apart from MukF/E, only one potential binding partner for MukB has been identified.…”