Condensation of vector DNA by the chromosomal protein HMG1 results in efficient transfection

Böttger, Michael; Vogel, Frank; Platzer, Matthias; Kießling, U.; Grade, K; Strauss, M

doi:10.1016/0167-4781(88)90014-0

Cited by 55 publications

(19 citation statements)

References 17 publications

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“…However, the mechanisms that enable HMGB1 to pass the cell membrane and the mitochondrial membranes still have to be clarified. Interestingly, HMGB1 has been used to potentiate the transfection of cells with compacted DNA, yielding greater transfection rates compared with conventional techniques (21). This might be due to the polybasic penetratin-like domain of HMGB1, common to proteins such as Antennapedia, lactoferrin, and the HIV protein Tat, which can transport DNA or RNA across cell and organelle membranes (1).…”

Section: Discussionmentioning

confidence: 99%

Danger Signaling Protein HMGB1 Induces a Distinct Form of Cell Death Accompanied by Formation of Giant Mitochondria

et al. 2010

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Cells dying by necrosis release the high-mobility group box 1 (HMGB1) protein, which has immunostimulatory effects. However, little is known about the direct actions of extracellular HMGB1 protein on cancer cells. Here, we show that recombinant human HMGB1 (rhHMGB1) exerts strong cytotoxic effects on malignant tumor cells. The rhHMGB1-induced cytotoxicity depends on the presence of mitochondria and leads to fast depletion of mitochondrial DNA, severe damage of the mitochondrial proteome by toxic malondialdehyde adducts, and formation of giant mitochondria. The formation of giant mitochondria is independent of direct nuclear signaling events, because giant mitochondria are also observed in cytoplasts lacking nuclei. Further, the reactive oxygen species scavenger N-acetylcysteine as well as c-Jun NH 2 -terminal kinase blockade inhibited the cytotoxic effect of rhHMGB1. Importantly, glioblastoma cells, but not normal astrocytes, were highly susceptible to rhHMGB1-induced cell death. Systemic treatment with rhHMGB1 results in significant growth inhibition of xenografted tumors in vivo. In summary, rhHMGB1 induces a distinct form of cell death in cancer cells, which differs from the known forms of apoptosis, autophagy, and senescence, possibly representing an important novel mechanism of specialized necrosis. Further, our findings suggest that rhHMGB1 may offer therapeutic applications in treatment of patients with malignant brain tumors.

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Section: Discussionmentioning

confidence: 99%

Danger Signaling Protein HMGB1 Induces a Distinct Form of Cell Death Accompanied by Formation of Giant Mitochondria

et al. 2010

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“…During the last 30 years non-viral gene transfer methods have been elaborated using nucleic acids associated with basic proteins [107,108], cationic polymers such as DEAE-dextran [109,110], polyornithine [111], polybrene [112], calcium phosphate precipitates [113][114][115], liposomes [116][117][118], cationic lipids [119,120], lipopolyamines [121,122], or electroporation [123,124] and microinjection [125]. General reviews are given by Felgner [126] and Cotten and Wagner [181].…”

Section: Viral and Non-viral Gene Transfer Methodsmentioning

confidence: 99%

Delivery of drugs, proteins and genes into cells using transferrin as a ligand for receptor-mediated endocytosis

Wagner

Curiel

Cotten

1994

Advanced Drug Delivery Reviews

259

128

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“…The use of HMG1 in gene-transfer complexes has been described in two different modes. First, HMG1-packaged plasmid DNA forms condensed complexes capable of transfecting cells with high efficiency (6). Second, HMG1 has been incorporated into complexes with liposomes, Sendai virus, erythrocyte membranes and plasmid DNA (17).…”

Section: Introductionmentioning

confidence: 99%

Recombinant HMG1 Protein Produced in Pichia pastoris : A Nonviral Gene Delivery Agent

Mistry¹,

Falciola²,

Monaco³

et al. 1997

BioTechniques

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Condensation of vector DNA by the chromosomal protein HMG1 results in efficient transfection

Cited by 55 publications

References 17 publications

Danger Signaling Protein HMGB1 Induces a Distinct Form of Cell Death Accompanied by Formation of Giant Mitochondria

Danger Signaling Protein HMGB1 Induces a Distinct Form of Cell Death Accompanied by Formation of Giant Mitochondria

Delivery of drugs, proteins and genes into cells using transferrin as a ligand for receptor-mediated endocytosis

Recombinant HMG1 Protein Produced in Pichia pastoris : A Nonviral Gene Delivery Agent

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