Concurrent quantification of tissue metabolism and blood flow via 2h/31P NMR in Viva iii. alterations of muscle blood flow and metabolism during sepsis

Song, Sheng‐Kwei; Hotchkiss, Richard S.; Karl, Irene E.; Ackerman, Joseph J. H.

doi:10.1002/mrm.1910250107

Cited by 25 publications

(3 citation statements)

References 29 publications

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“…The rate of tissue uptake of D,O following intravenous bolus injection is widely used to measure perfusion rate (in milliliters/100 g of tissue x minutes) because it is approximately fully extracted ( E = 1.0) from the capillary bed during a single passage (i.e., during the mean transit time). A large body of work (e.g., [11][12][13][14] validates the use of magnetic resonance detection of D,O uptake for accurate perfusion measurements. Kim and Ackerman et 01. measured perfusion rates in rodent kidney, liver, brain, muscle and tumor (11.…”

Section: Introductionmentioning

confidence: 98%

In vivo imaging of extraction fraction of low molecular weight mr contrast agents and perfusion rate in rodent tumors

Kovar

Lewis

River

et al. 1997

Magnetic Resonance in Med

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Tissue uptake of a fully extractable MR detectable tracer, deuterated water (D2O), was compared with that of a less extractable contrast agent, Gadolinium-DTPA-dimeglumine (Gd-DTPA), in rodent tumor and muscle tissue. This dual tracer method allowed calculation of relative (to muscle) tissue perfusion and extraction fraction of Gd-DTPA in each image pixel in vivo. Solutions of Gd-DTPA and D2O were injected intravenously into Fisher female rats (n = 9) with R3230 mammary adenocarcinomas implanted in the hind limb. Perfusion rate was approximately two times greater (P < 0.005 by paired t test) in tumor than in muscle. Gd-DTPA extraction fraction at the interface between tumor and muscle was 2.0 times the extraction fraction in normal muscle (P < 0.005 by paired t test). Extraction fraction at the tumor center was 1.6 times the extraction fraction in muscle (P < 0.01 by paired t test). High extraction fraction of Gd-DTPA correlated with high capillary permeability determined from Evans Blue staining. Low molecular weight Gd-DTPA derivatives are widely used in clinical practice, and their extraction fractions are crucial determinants of image contrast during the first few passes of the contrast agent bolus. Therefore spatially resolved measurements of contrast agent extraction fractions obtained in vivo have significant clinical utility. The data demonstrate that extraction of low molecular weight tracers is sensitive to increased permeability in tumor vasculature and that this increased permeability can be imaged.

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Section: Introductionmentioning

confidence: 98%

In vivo imaging of extraction fraction of low molecular weight mr contrast agents and perfusion rate in rodent tumors

Kovar

Lewis

River

et al. 1997

Magnetic Resonance in Med

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“…The relationship between intracellular sodium accumulation and high-energy phosphate depletion was demonstrated during rat leg ischemia by 23 Na/ 31 P spectroscopy (7). The possibility of concurrent quantification of tissue metabolism and blood flow via 2 H/ 31 P NMR was shown and validated in the rat and was applied to investigate an experimental model of sepsis (8). Surprisingly, and rather disappointingly, this did not develop into a plethora of applications.…”

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confidence: 99%

How to investigate oxygen supply, uptake, and utilization simultaneously by interleaved NMR imaging and spectroscopy of the skeletal muscle

Carlier

Brillault-Salvat

Giacomini

et al. 2005

Magnetic Resonance in Med

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Human skeletal muscle perfusion, oxygenation, and high-energy phosphate distribution were measured simultaneously by interleaved 1 H and 31 P NMR spectroscopy and 1 H NMR imaging in vivo. From these parameters, arterial oxygen supply (DO 2 ), muscle reoxygenation rate, mitochondrial ATP production, and O 2 consumption (VO 2 ) were deduced at the recovery phase of a short ischemic exercise bout. In addition, by using a reformulation of the mass conservation law, muscle maximum O 2 extraction was calculated from these parameters. Perhaps even more appealing than its non-invasiveness is the ability of NMR to investigate a great many facets of physiology and biochemistry in vivo. In peripheral organs, it is possible to explore by NMR techniques: muscle kinematics, mass and composition, vascularization, regional perfusion, blood and tissue oxygenation, high-energy phosphate distribution, lactate production and intracellular pH, Krebs cycle activity, and mitochondrial oxidative phosphorylation. Regrettably, NMR acquisition schemes rarely explore more than a limited number of parameters, often one at a time, essentially because of the quite specific spectrometer configuration required for each particular examination type. This has been a handicap in the study of tissue regulation in vivo, which ought to be a major target for NMR biomedical applications.This drawback is well illustrated in one study aimed at elucidating the relationship between skeletal muscle perfusion and oxidative phosphorylation (1). Due to technical constraints, post-ischemic reactive hyperemia and creatine rephosphorylation rate were measured during separate experimental sessions. As a consequence, the modest though highly significant correlation between the two parameters did not clarify the question under consideration. Did the observed, relatively weak, degree of dependence of oxidative phosphorylation on perfusion translate a biologic reality, or did it essentially reflect inter-study variability?One possible solution, instigated by a few groups, was the implementation of other techniques inside the magnet bore, to be run in parallel to the NMR data acquisition. Near infrared spectroscopy (NIRS) was combined to 31 P NMR spectroscopy as an elegant way to investigate further the oxygen dependence of high-energy phosphate production (2). If NIRS offers many practical advantages, such as high temporal resolution, clinical applicability, and favorable cost-effectiveness, it also suffers serious limitations, mainly related to ambiguous spatial and molecular origins of the re-emitted signal. Though more challenging, NMR seems to bear a greater potential in terms of discriminating capillary and cell oxygenation (3), and developing multiparametric NMR protocols is a valid, possibly preferable, option.Concurrent heteronuclear NMR was pioneered both in Oxford and in Philadelphia (4,5) and culminated with the demonstration of simultaneous in vivo 1 H, 19 F, 31 P, and 23 Na NMR acquisitions (6). The relationship between intracellular sodium accumulation an...

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“…From the 1980s until the present, applications of flow NMR have rapidly spread, including the biological sciences. − For example, in vivo 31 P flow NMR studies 14 of ATP metabolites in blood have been reported. Similar recycled-flow NMR experiments with other high-sensitivity nuclei (e.g., 2 H, 19 F, etc.)…”

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confidence: 99%

¹³C Dynamic Nuclear Polarization: an Alternative Detector for Recycled-Flow NMR Experiments

Stevenson

Glass

1998

Anal. Chem.

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Static NMR experiments on insensitive nuclei (e.g., (13)C) have been generally difficult because of low magnetogyric ratios and/or poor natural abundance, even at the highest magnetic fields available. One method of overcoming these sensitivity limitations is dynamic nuclear polarization (DNP), which can be utilized to enhance NMR signals relative to those obtained with static NMR detection. In this study, we report a recycled-flow DNP apparatus capable of monitoring (13)C scalar-dominated enhancements 1-2 orders of magnitude greater than those obtainable using the conventional recycled-flow (13)C NMR experiment. Specifically, a mixture of benzene and several chlorocarbons were continuously "recycled" through a (13)C DNP spectrometer. Results indicate successful detection with the (13)C DNP approach in scalar-dominated cases, where analytes could not be observed with conventional static or flow NMR detection in a reasonable length of time.

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Concurrent quantification of tissue metabolism and blood flow via ²h/³¹P NMR in Viva iii. alterations of muscle blood flow and metabolism during sepsis

Cited by 25 publications

References 29 publications

In vivo imaging of extraction fraction of low molecular weight mr contrast agents and perfusion rate in rodent tumors

In vivo imaging of extraction fraction of low molecular weight mr contrast agents and perfusion rate in rodent tumors

How to investigate oxygen supply, uptake, and utilization simultaneously by interleaved NMR imaging and spectroscopy of the skeletal muscle

¹³C Dynamic Nuclear Polarization: an Alternative Detector for Recycled-Flow NMR Experiments

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Concurrent quantification of tissue metabolism and blood flow via 2h/31P NMR in Viva iii. alterations of muscle blood flow and metabolism during sepsis

Cited by 25 publications

References 29 publications

In vivo imaging of extraction fraction of low molecular weight mr contrast agents and perfusion rate in rodent tumors

In vivo imaging of extraction fraction of low molecular weight mr contrast agents and perfusion rate in rodent tumors

How to investigate oxygen supply, uptake, and utilization simultaneously by interleaved NMR imaging and spectroscopy of the skeletal muscle

13C Dynamic Nuclear Polarization: an Alternative Detector for Recycled-Flow NMR Experiments

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Concurrent quantification of tissue metabolism and blood flow via ²h/³¹P NMR in Viva iii. alterations of muscle blood flow and metabolism during sepsis

¹³C Dynamic Nuclear Polarization: an Alternative Detector for Recycled-Flow NMR Experiments