Concurrent production and relative quantification of vasicinone from in vivo and in vitro plant parts of Malabar nut (Adhatoda vasica Nees)

Abstract

The present study documents a simultaneous production and comparative assessment of extracted vasicinone from in vivo (leaves and stems) and in vitro (leaves, stems and calli) plant parts of Adhatoda vasica Nees, a well-known medicinal plant. High-performance thin layer chromatography (HPTLC) analysis of the above-mentioned plant parts, collected at their 60-day-old growth stage, was performed via methanolic extraction and with the aid of toluene:butanol:butyl acetate (9:0.5:0.5; v/v/v) solvent system. The method was validated with the help of aluminium sheet precoated with silica gel 60 F254 TLC plates, following the ICH guidelines in order to maintain accuracy, precision and repeatability. Correlation coefficient, limit of detection and limit of quantification values were found to be reasonable. The outcome revealed a linearity that ranged between 2 and 6 µg/spot. During the comparison of estimated vasicinone quantity from in vivo and in vitro plant parts, it was evident that in vitro samples produced relatively higher vasicinone than that of the in vivo counterparts. Maximum vasicinone (6.402 ± 0.010% of dry weight) production was quantified from in vitro leaves followed by calli (5.222 ± 0.092% of dry weight) and in vitro stems (2.007 ± 0.041% of dry weight). On the other hand, in vivo leaves and stems produced comparatively lower quantities of vasicinone (2.412 ± 0.139 and 1.933 ± 0.046% of dry weight, respectively) suggesting the in vitro clonal propagation as a superior approach in comparison to in vivo propagation. Nonetheless, simultaneous production from both the sources (in vivo and in vitro plant parts) provides a new avenue for augmented production of vasicinone.

Ameliorated reserpine production via in vitro direct and indirect regeneration system in Rauvolfia serpentina (L.) Benth. ex Kurz.

“…On the other hand, calli have an unorganized constitution, wherein not all cells might be active and hence are oriented towards a defined development and secondary metabolite production. The obtained result (reserpine content of the mother plant) of the present experiment corresponds with the reports of Panigrahi et al (2017), wherein it was mentioned that in vitro cultured plant parts contain significantly higher secondary metabolites than that of the same ex vitro plant parts. For instance, a lower value of reserpine from ex vitro leaves (13.77 μg) was reported by Panda et al (2010).…”

Chyawanprash: A Traditional Indian Bioactive Health Supplement

“…The dominant ingredient is Amla , a citrus fruit that is a highly renowned and potent botanical in Ayurveda. The main ingredients of CP, along with their botanical identities, key active biomolecules and specific therapeutic roles, are detailed in Figure 1 and Table 1 [12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55].…”

In vitro biotechnological advancements in Malabar nut (Adhatoda vasica Nees): Achievements, status and prospects

“…Similar trends was observed by Madhukar et al [40], when they assessed the callus culture of A. vasica via ultra-performance liquid chromatography/quadrupole-time-of-flight mass-spectrometry (UPLC/Q‑TOF‑MS), wherein, 123.3% increase in vasicine content was observed, compared to control plantlets. However, only a single report was documented, concerning the production of vasicinone, both from in vivo and in vitro plant parts of A. vasica [59]. In that report, the maximum vasicinone content (6.402% of dry weight) was obtained from in vitro leaf, followed by in vitro shoot (2.007% of dry weight), making way for simultaneous production of vasicinone more efficiently.…”