Concurrent overexpression of amino acid permease

Grant, J. E.; Ninan, Annu Smitha; Cripps-Guazzone, Natalia; Shaw, Martin; Song, Jiancheng; Petřík, Ivan; Novák, Ondřej; Tegeder, Mechthild; Jameson, Paula E.

doi:10.1071/fp21011

Cited by 18 publications

(25 citation statements)

References 77 publications

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“…Amino acid transporters are triggered to a greater extent by the applied NH 4 + /NO 3 − ratios in the soil as compared to the hydroponic system. The amino acid profile were influenced by different N application forms, which showed a great impact on the contents of amino acids in different plant parts [ 80 , 81 ]. The most important function of amino acid transporters is the movement of amino acids from source to sink, i.e., plant leaves and seeds to fruits and the exertion of a regulatory mechanism for the uptake of N, the synthesis of amino acids and the allocation of amino acids in plants [ 79 , 81 ].…”

Section: Discussionmentioning

confidence: 99%

“…The amino acid profile were influenced by different N application forms, which showed a great impact on the contents of amino acids in different plant parts [ 80 , 81 ]. The most important function of amino acid transporters is the movement of amino acids from source to sink, i.e., plant leaves and seeds to fruits and the exertion of a regulatory mechanism for the uptake of N, the synthesis of amino acids and the allocation of amino acids in plants [ 79 , 81 ]. Positive feedback of N assimilation helped to increase the N uptake from the solution to roots and roots to shoots due to the applied NH 4 + /NO 3 − ratios and ultimately increased the various types of amino acids in the soil as compared to the hydroponic cultivation system.…”

Section: Discussionmentioning

confidence: 99%

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Differential Metabolic Responses of Lettuce Grown in Soil, Substrate and Hydroponic Cultivation Systems under NH4+/NO3− Application

et al. 2022

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Nitrogen (N) is an essential element for plant growth and development. The application of a balanced and optimal amount of N is required for sustainable plant yield. For this, different N sources and forms are used, that including ammonium (NH4+) and nitrate (NO3−). These are the main sources for N uptake by plants where NH4+/NO3− ratios have a significant effect on the biomass, quality and metabolites composition of lettuce grown in soil, substrate and hydroponic cultivation systems. A limited supply of N resulted in the reduction in the biomass, quality and overall yield of lettuce. Additionally, different types of metabolites were produced with varying concentrations of N sources and can be used as metabolic markers to improve the N use efficiency. To investigate the differential metabolic activity, we planted lettuce with different NH4+/NO3− ratios (100:0, 75:25, 50:50, 25:75 and 0:100%) and a control (no additional N applied) in soil, substrate and hydroponic cultivation systems. The results revealed that the 25% NH4+/75% NO3− ratio increased the relative chlorophyll contents as well as the biomass of lettuce in all cultivation systems. However, lettuce grown in the hydroponic cultivation system showed the best results. The concentration of essential amino acids including alanine, valine, leucine, lysine, proline and serine increased in soil and hydroponically grown lettuce treated with the 25% NH4+/75% NO3− ratio. The taste and quality-related compounds in lettuce showed maximum relative abundance with the 25% NH4+/75% NO3− ratio, except ascorbate (grown in soil) and lactupicrin (grown in substrate), which showed maximum relative abundance in the 50% NH4+/50% NO3− ratio and control treatments, respectively. Moreover, 1-O-caffeoylglucose, 1,3-dicaffeoylquinic acid, aesculetin and quercetin-3-galactoside were increased by the application of the 100% NH4+/0% NO3− ratio in soil-grown lettuce. The 25% NH4+/75% NO3− ratio was more suitable in the hydroponic cultivation system to obtain increased lettuce biomass. The metabolic profiling of lettuce showed different behaviors when applying different NH4+/NO3− ratios. Therefore, the majority of the parameters were largely influenced by the 25% NH4+/75% NO3− ratio, which resulted in the hyper-accumulation of health-promoting compounds in lettuce. In conclusion, the optimal N applications improve the quality of lettuce grown in soil, substrate and hydroponic cultivation systems which ultimately boost the nutritional value of lettuce.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Differential Metabolic Responses of Lettuce Grown in Soil, Substrate and Hydroponic Cultivation Systems under NH4+/NO3− Application

et al. 2022

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“…In a pea mutant overexpressing SUT1 sucrose transporter and AAP1 amino‐acid transporter, differential expression of PsCWINV1.2 was observed (Grant et al, 2021). Grant et al (2021) also reported co‐upregulated expression of PsCWINV1.2 with a suite of sucrose transporting clade III SWEETs in pea seed coat. Thus, they provided an insight into how PsCWINV1.2 expression in seed coats could be upregulated by a source‐driven influx of sugars and amino acids.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, sugar flux is partly controlled through the catalytic activity of CWINV localized to the seed coat, determining the magnitude of the transmembrane sucrose gradient. In a pea mutant overexpressing SUT1 sucrose transporter and AAP1 amino‐acid transporter, differential expression of PsCWINV1.2 was observed (Grant et al, 2021). Grant et al (2021) also reported co‐upregulated expression of PsCWINV1.2 with a suite of sucrose transporting clade III SWEETs in pea seed coat.…”

Section: Discussionmentioning

confidence: 99%

Genome‐wide identification of invertases in Fabaceae, focusing on transcriptional regulation of Pisum sativum invertases in seed subjected to drought

Morin

Kadi

Porcheron

et al. 2022

Physiologia Plantarum

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Invertases are key enzymes for carbon metabolism, cleaving sucrose into energy‐rich and signaling metabolites, glucose and fructose. Invertases play pivotal roles in development and stress response, determining yield and quality of seed production. In this context, the repertoire of invertase gene families is critically scarce in legumes. Here, we performed a systematic search for invertase families in 16 Fabaceae genomes. For instance, we identified 19 invertase genes in the model plant Medicago and 17 accessions in the agronomic crop Pisum sativum. Our comprehensive phylogenetic analysis sets a milestone for the scientific community as we propose a new nomenclature to correctly name plant invertases. Thus, neutral invertases were classified into four clades of cytosolic invertase (CINV). Acid invertases were classified into two cell wall invertase clades (CWINV) and two vacuolar invertase clades (VINV). Then, we explored transcriptional regulation of the pea invertase family, focusing on seed development and water stress. Invertase expression decreased sharply from embryogenesis to seed‐filling stages, consistent with higher sucrose and lower monosaccharide contents. The vacuolar invertase PsVINV1.1 clearly marked the transition between both developmental stages. We hypothesize that the predominantly expressed cell wall invertase, PsCWINV1.2, may drive sucrose unloading towards developing seeds. The same candidates, PsVINV1.1 and PsCWINV1.2, were also regulated by water deficit during embryonic stage. We suggest that PsVINV1.1 along with vacuolar sugar transporters maintain cellular osmotic pressure and PsCWINV1.2 control hexose provision, thereby ensuring embryo survival in drought conditions. Altogether, our findings provide novel insights into the regulation of plant carbon metabolism in a challenging environment.

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“…Similar to the study by Kaur et al [ 101 ], which investigated interactions between N and C metabolism, a study was conducted to identify interactions between N and C transport. For this purpose, they first obtained homozygous pea lines overexpressing a sucrose transporter ( PsSUT1 ) or an amino acid transporter ( PsAAP1(3a) ) gebes and then crossed them [ 144 ]. The transgenes contributed to transition of young leaves from sink to source state, and the protein content and seed yield in the double transformants were higher than in the single ones.…”

Section: Genetic Engineering Of Nitrogen Metabolismmentioning

confidence: 99%

Genetic Engineering and Genome Editing for Improving Nitrogen Use Efficiency in Plants

Lebedev

Popova

Shestibratov

2021

Cells

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Low nitrogen availability is one of the main limiting factors for plant growth and development, and high doses of N fertilizers are necessary to achieve high yields in agriculture. However, most N is not used by plants and pollutes the environment. This situation can be improved by enhancing the nitrogen use efficiency (NUE) in plants. NUE is a complex trait driven by multiple interactions between genetic and environmental factors, and its improvement requires a fundamental understanding of the key steps in plant N metabolism—uptake, assimilation, and remobilization. This review summarizes two decades of research into bioengineering modification of N metabolism to increase the biomass accumulation and yield in crops. The expression of structural and regulatory genes was most often altered using overexpression strategies, although RNAi and genome editing techniques were also used. Particular attention was paid to woody plants, which have great economic importance, play a crucial role in the ecosystems and have fundamental differences from herbaceous species. The review also considers the issue of unintended effects of transgenic plants with modified N metabolism, e.g., early flowering—a research topic which is currently receiving little attention. The future prospects of improving NUE in crops, essential for the development of sustainable agriculture, using various approaches and in the context of global climate change, are discussed.

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Concurrent overexpression of amino acid permease

Cited by 18 publications

References 77 publications

Differential Metabolic Responses of Lettuce Grown in Soil, Substrate and Hydroponic Cultivation Systems under NH4+/NO3− Application

Differential Metabolic Responses of Lettuce Grown in Soil, Substrate and Hydroponic Cultivation Systems under NH4+/NO3− Application

Genome‐wide identification of invertases in Fabaceae, focusing on transcriptional regulation of Pisum sativum invertases in seed subjected to drought

Genetic Engineering and Genome Editing for Improving Nitrogen Use Efficiency in Plants

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