Concurrent nicotine exposure to prenatal alcohol consumption alters the hippocampal and cortical neurotoxicity

Bhattacharya, Dwipayan; Fujihashi, Ayaka; Majrashi, Mohammed; Bloemer, Jenna; Bhattacharya, Subhrajit; Buabeid, Manal; Escobar, Martha; Moore, Timothy; Suppiramaniam, Vishnu; Dhanasekaran, Muralikrishnan

doi:10.1016/j.heliyon.2019.e03045

Cited by 13 publications

(11 citation statements)

References 46 publications

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“…The AchE activities in all treatment groups increased significantly, which could have a consequential decrease in cognitive function [24]. Ach is among the central neurotransmitters, which play a significant role in memory, learning and cognition [25].…”

Section: Discussionmentioning

confidence: 98%

Herbal snuff (AK-47 and HAM) induce oxidative stress and increase acetylcholinesterase enzyme activity in rat brain

Muhammad¹,

Barau²,

Zaruwa³

et al. 2021

GSC Biol. and Pharm. Sci.

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Snuff has resurfaced not only in western countries but in Africa including Nigeria. It is now almost generally acceptable, among young and old in Nigeria. This research was designed to investigate the Effect of Hajiya Aisha Manpower (HAM) and AK-47 on Antioxidant Status and Acetylcholinesterase enzyme activity (AchE) of Wister Albino Rats. Thirty (30) Wister rats (110-120 g) were arbitrarily divided into five groups. Group1 (control); received only distilled water. Groups 2 and 3 (received 6 mg and 3 mg/kg b.w.t of HAM respectively). Groups 4 and 5 (received 6 mg and 3 mg/kg b.w.t AK-47 of respectively). After two months of treatments, the rats were anesthetized, blood samples were taken through heart puncture and brains of all rats were isolated and homogenized. The Result revealed Non-significant decrease in Superoxide dismutase (SOD), glutathione peroxidase (GPx) activities and concomitant increase in GSH levels in treatment groups were observed in relation to the control. While a substantial increase (p˂ 0.5) in MDA was detected in treatment groups. Brain AchE activity increased significantly in all treatment groups in relation to the control. We conclude that Both AK-47 and HAM at high concentration induce oxidative stress, decreased antioxidant enzyme activities and promote degradation of acetylcholine in rat brain homogenate.

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Section: Discussionmentioning

confidence: 98%

Herbal snuff (AK-47 and HAM) induce oxidative stress and increase acetylcholinesterase enzyme activity in rat brain

Muhammad¹,

Barau²,

Zaruwa³

et al. 2021

GSC Biol. and Pharm. Sci.

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“…Another study also showed that alcohol toxification is lower in chronic smokers compared to non-smokers 87 . Recently, Bhattacharya et al 18 showed that prenatal ethanol exposure (10% v/v) could increase ROS production, LP, and caspases activities and also decrease spatial memory in F1 rats, while simultaneous exposure to ethanol and nicotine (6 mg/kg/day, by mini pump) could alleviate the adverse effects of ethanol on OS and hippocampal memory. However, they exposed pregnant rats to nicotine and ethanol at higher and lower doses, respectively, compared with the current study.…”

Section: Discussionmentioning

confidence: 99%

“…It is well-known that consumption of any of these substances increase the desire for the other 17 . To date, some studies have investigated the effect of prenatal co-exposure to these substances on various tissue, but they have yielded conflicting results 18 – 21 . However, no study has evaluated the effect of developmental co-exposure to alcohol and nicotine on the testicular properties and sperm parameters of male offspring.…”

Section: Introductionmentioning

confidence: 99%

Genetic and epigenetic modifications of F1 offspring’s sperm cells following in utero and lactational combined exposure to nicotine and ethanol

Pabarja

Hakemi

Musanejad

et al. 2021

Sci Rep

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It is well established that maternal lifestyle during pregnancy and lactation affects the intrauterine programming of F1 offspring. However, despite the co-use of alcohol and nicotine is a common habit, the effects of exposure to both substances on the reproductive system of F1 male offspring and the underlying mechanisms of developmental programming have not been investigated. The present study aimed to examine pre- and postnatal concurrent exposure to these substances on genetic and epigenetic alterations of sperm cells as well as testis properties of F1 offspring compared with exposure to each substance alone. Pregnant dams in the F0 generation randomly received normal saline, nicotine, ethanol, and combinations throughout full gestation and lactation periods. Sperm cells and testes of F1 male offspring were collected at postnatal day 90 for further experiments. High levels of sperm DNA fragmentation were observed in all exposed offspring. Regarding epigenetic alterations, there was a significant increase in the relative transcript abundance of histone deacetylase 1 and 2 in all exposed sperm cells. Moreover, despite a decrease in the expression level of DNA methyltransferase (DNMT) 3A, no marked differences were found in the expression levels of DNMT1 and 3B in any of the exposed sperm cells compared to non-exposed ones. Interestingly, combined exposure had less prominent effects relative to exposure to each substance alone. The changes in the testicular and sperm parameters were compatible with genetic and epigenetic alterations. However, MDA level as an oxidative stress indicator increased in all exposed pups, which may be responsible for such outputs. In conclusion, maternal co-exposure to these substances exhibited epigenotoxicity effects on germline cells of F1 male offspring, although these effects were less marked relative to exposure to each substance alone. These counteracting effects may be explained by cross-tolerance and probably less impairment of the antioxidant defense system.

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“…Another study also showed that alcohol toxi cation is lower in chronic smokers compared to that of the non-smokers 60 . Recently, Bhattacharya and colleagues 61 showed that prenatal ethanol exposure (10% v/v) could increase ROS production, LP, caspases activities as well as decrease spatial memory in F1 rats, while simultaneous exposure to ethanol and nicotine (6 mg /kg/day, by mini pump) could alleviate the adverse effects of ethanol on oxidative stress and hippocampal memory. However, they exposed pregnant rats to nicotine and ethanol at higher and lower doses, respectively compared to our study.…”

Section: Discussionmentioning

confidence: 99%

In Utero and Lactational Exposure of F1 Adult Pups to Ethanol and Nicotine: Alteration of The Reproductive System and Epigenetic Modifying Enzymes of Sperm Cells?

Pabarja¹,

Hakemi²,

Musanejad³

et al. 2021

Preprint

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Co-use of alcohol and nicotine is a common habit which could affect reproductive system. The present study was designed to investigate the effect of in-utero and lactational exposure of offspring to alcohol, nicotine, and combinations on the reproductive system and epigenetic alterations of male gamete. Pregnant mice and lactating NMRI pups randomly received nicotine, ethanol, and combinations during gestational days 1 until weaning. Sperm and testes were collected on postnatal day 90 for further experiments. Exposure to these substances, particularly, nicotine, highly affected testicular Johnsen’s score, sperm parameters including; number, motility, viability, DNA integrity and also serum MDA level. Interestingly, concurrent exposure to nicotine and alcohol somehow reversed the effects. Quantitative real-time PCR data showed an increase in the mRNA level of histone deacetylation 1 and 2 and a decrease in the level of DNA methyltransferase (DNMT) 3A, while no marked differences in the expression level of DNMT1 and 3B were observed between the exposed and non-exposed pups. Alleviating effects were observed in the epigenetic modifying enzymes transcripts of co-exposed pups compared with the exposure to nicotine or alcohol. Taken together, our findings determined that exposure of offspring in-utero and during lactational period to these substances could result in lasting epigenetic changes in sperm cells. However, unexpectedly co-exposure of pups to nicotine and alcohol lessened these negative effects.

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Concurrent nicotine exposure to prenatal alcohol consumption alters the hippocampal and cortical neurotoxicity

Cited by 13 publications

References 46 publications

Herbal snuff (AK-47 and HAM) induce oxidative stress and increase acetylcholinesterase enzyme activity in rat brain

Herbal snuff (AK-47 and HAM) induce oxidative stress and increase acetylcholinesterase enzyme activity in rat brain

Genetic and epigenetic modifications of F1 offspring’s sperm cells following in utero and lactational combined exposure to nicotine and ethanol

In Utero and Lactational Exposure of F1 Adult Pups to Ethanol and Nicotine: Alteration of The Reproductive System and Epigenetic Modifying Enzymes of Sperm Cells?

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