Concurrent Infection in a Dog and Colonization in a Child with a Human Enteropathogenic
            <i>Escherichia coli</i>
            Clone

Rodrigues, Josias; Thomazini, Cristiane Melissa; Lopes, C. A.; Dantas, Liliane O.

doi:10.1128/jcm.42.3.1388-1389.2004

Cited by 30 publications

(24 citation statements)

References 11 publications

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“…Dogs live in close contact with humans and direct transmission of E. coli strains is a probable occurrence. RODRIGUES et al (2004) demonstrated cross-infection between a dog and a child that lived in the same home in a city of the state of São Paulo, Brazil. Hemolytic E. coli are a common occurrence both in healthy and in dogs with intestinal and extraintestinal infections (BEUTIN, 1999).…”

Section: Discussionmentioning

confidence: 99%

“…Pets can be natural reservoirs of several organisms potentially able to cause disease to humans; children in special are central players in this crosstransfer game in view of their frequent nonobservance of proper hygiene habits (RODRIGUES et al, 2004). The eventual role of dogs as reservoirs of STEC strains has not been totally elucidated.…”

Section: Introductionmentioning

confidence: 99%

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Occurrence of non-O157 Shiga toxin-producing Escherichia coli in dogs with diarrhea

Paula

Marin

2008

Cienc. Rural

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Occurrence of non-O157 Shiga toxin-producing Escherichia coli in dogs with diarrhea

Paula

Marin

2008

Cienc. Rural

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“…Libraries are intended to be collections of known-host enteric bacteria which are presumably host specific. We are aware of no means to distinguish enteric bacteria, which have a special niche in the intestinal tracts of particular hosts (21), from those which are transient or cosmopolitan (existing in multiple hosts) in nature (20,23,25); thus, many fecal isolates may be misclassified in host source libraries.…”

mentioning

confidence: 99%

Specificity of a Bacteroides thetaiotaomicron Marker for Human Feces

Carson¹,

Christiansen²,

Yampara-Iquise³

et al. 2005

Appl Environ Microbiol

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“…Rodrigues et al 15 reported that DEPEC isolated from a pup with diarrhea were closely related to human EPEC. They also studied samples isolated from a child and noticed a phenotypic and genetic similarity to the same serotype, as well as identical EPEC markers.…”

Section: Discussionmentioning

confidence: 99%

Experimental infection with enteropathogenic Escherichia coli identified by PCR using enteric-coated capsules in boxer pups

Gouveia¹,

Silva²,

Nakazato

et al. 2011

Acta Cir. Bras.

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Purpose:To verify the possibility of an experimental infection with enteropathogenic Escherichia coli and to confirm by PCR that the symptoms manifested after infection were due to the virulence factors of the studied bacteria. Methods: Experimental units were 14 healthy pups of Boxer breed, aged 60 days. The animals were divided into three groups. One animal from each litter was included in a control group and the remaining animals were divided into two groups: one inoculated with strain 4083, and another one inoculated with strain SPA14. Gelatinous capsules coated with enteric-coating solution were used for the inoculation of strains. E. coli isolation from feces was performed for all tested animals, and the extracted DNA was subjected to Polymerase Chain Reaction (PCR). Results: All infected animals presented diarrhea and had the gene eae amplified by PCR. Conclusion: The efficiency of PCR for the studied strains indicates that this technique can be recommended for the diagnosis of enteropathogenic Escherichia coli as a differential from other pathogens causing diarrhea. It may also be used in the future to verify whether other virulence factors (bfpA gene and EAF plasmid) persist after infection and to assess the pathogenicity of these bacteria. Key words: Enteropathogenic Escherichia coli. Virulence Factors. Polymerase Chain Reaction. Dogs. RESUMOObjetivo: Verificar a possibilidade de uma infecção experimental com Escherichia coli enteropatogênicas e confirmar por PCR que os sintomas manifestados após a infecção foram decorrentes dos fatores de virulência da bactéria estudada. Métodos: As unidades experimentais foram 14 filhotes saudáveis com idade de 60 dias da raça Boxer. Os animais foram divididos em três grupos, sendo um controle de cada ninhada e o restante dividido em dois grupos, um de animais inoculados com a cepa 4083 e o outro de animais inoculados com a cepa SPA14. Para inoculação das cepas, utilizaram-se cápsulas gelatinosas revestidas com solução de revestimento entérico. O isolamento de E. coli das fezes foi realizado em todos os animais testados, e o DNA extraído foi submetido à técnica de PCR. Resultados: Todos os animais infectados apresentaram diarréia e tiveram a gene eae amplificado por meio de PCR. Conclusão: Através da eficiência da PCR das amostras, a técnica seria recomendada para diagnóstico da Escherichia coli enteropatogênicas como diferencial de outros patógenos que causam diarréia, e, no futuro, verificar se outros fatores de virulência (gene bfpA e plasmídeo EAF) permaneceriam após a infecção, podendo avaliar a patogenicidade das EPEC. Descritores: Escherichia coli Enteropatogênica. Fatores de Virulência. Reação em Cadeia da Polimerase. Cães.

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Concurrent Infection in a Dog and Colonization in a Child with a Human Enteropathogenic Escherichia coli Clone

Cited by 30 publications

References 11 publications

Occurrence of non-O157 Shiga toxin-producing Escherichia coli in dogs with diarrhea

Occurrence of non-O157 Shiga toxin-producing Escherichia coli in dogs with diarrhea

Specificity of a Bacteroides thetaiotaomicron Marker for Human Feces

Experimental infection with enteropathogenic Escherichia coli identified by PCR using enteric-coated capsules in boxer pups

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