Concurrent Fluorescence &lt;i&gt;in Situ &lt;/i&gt;Hybridization and Immunocytochemistry for the Detection of Chromosome Aberrations in Exfoliated Bronchial Epithelial Cells

Neft, Robin E.; Murphy, Minerva M.; Tierney, Lauren A.; Belinsky, Steven A.; Anderson, Marshall W.; Saccomanno, Geno; Michels, Ruth; Timm, Susan; Gilliland, Frank D.; Crowell, Richard E.; Lechner, John F.

doi:10.1159/000333183

Cited by 6 publications

(4 citation statements)

References 5 publications

(5 reference statements)

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“…The Megafunnel technique provided an alternative method of slide preparation for immunocytochemistry, 2 genetic analysis and fluorescence in situ hybridization (FISH) analysis. 16 Multiple Megafunnel slides can be made using cells removed from archival Saccomanno smear slides for use in molecular assays for several retrospective studies on the same specimen, preserving a valuable, irreplaceable resource for future studies. Slide-by-slide comparison of the cytologic diagnosis for sputum samples emphasized the heterogeneity of these specimens.…”

Section: Discussionmentioning

confidence: 99%

Saccomanno Smear Slides and Megafunnel Slides for Sputum Specimens

Michels¹,

Davidson²,

Timm³

et al. 1997

Acta Cytologica

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Section: Discussionmentioning

confidence: 99%

Saccomanno Smear Slides and Megafunnel Slides for Sputum Specimens

Michels¹,

Davidson²,

Timm³

et al. 1997

Acta Cytologica

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“…Chromosome copy changes have been reported in the normal bronchial epithelium of lung cancer patients [136][137][138], but most fluorescence in situ hybridisation (FISH) studies have used centromeric probes, which give good signals but may not be sufficiently informative for tumour-specific changes [139][140][141]. A more specific set of probes is required to provide better discrimination between general epithelial disturbance and actual tumour development.…”

Section: Molecular Cytogenetic Analysis Of Lung Tumoursmentioning

confidence: 99%

The Liverpool Lung Project: a molecular epidemiological study of early lung cancer detection

Field¹,

Youngson²

2002

Eur Respir J

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Lung cancer is the major cause of death from neoplastic disease in the world, and even with politically-motivated smoking cessation campaigns throughout Europe, the disease remains the major cause of death. The development of molecular epidemiological population-based research into early lung cancer detection, such as the Liverpool Lung Project (LLP), may provide a way forward. This is the first major molecular epidemiological study of detection of early lung cancer.The use of molecular epidemiological risk assessments prior to clinical diagnosis and markers of preclinical carcinogenesis in patients with a high risk of developing lung cancer will reduce the incidence of clinically-detectable lung cancer, given the appropriate intervention strategies.The aims are as follows: 1) to prepare a molecular genetic and epidemiological risk assessment model based on environmental exposures and genetic predisposition; 2) to develop an archive of specimens relating to at-risk individuals and those with lung cancer; 3) to redefine lung cancer based on molecular pathology using the fields of expression profiling, genetic instability and molecular cytogenetics; 4) to identify and assess novel markers of precarcinogenesis in high-risk populations; and 5) to facilitate the development of new treatment strategies (e.g. chemoprevention programmes and targeted drug therapies).The LLP has two components: 1) a case-controlled study of newly-diagnosed cases of lung cancer that will provide a baseline, risk assessment; and 2) a prospective cohort study to be carried out over a 10-yr period that will identify markers of preclinical carcinogenesis. In-depth interviews are carried out using structured and semi-structured questionnaires. Sputum, blood and tumour specimens are collected and will be assessed for specific molecular markers (e.g. genetic instability, mutation and expression profiling, and methylation status).Conclusions from The Liverpool Lung Project will be based around molecularepidemiological and genotyping risk assessment models, as well as redefining the disease, and ultimately contributing to the development of new early lung cancer detection and treatment strategies.

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“…1997;41:529-532 Thomas S (see Ashfaq et al). 1997;41:1058-1064 Tierney LA (see Neft et al). 1997;41:1769-1773 Tiesi C (see Hawthorne et al).…”

mentioning

confidence: 99%

“…1997;41:1769-1773 Tiesi C (see Hawthorne et al). 1997;41:160-165 Timm S (see Neft et al). 1997;41:1769-1773 Timm SS (see Michels et al).…”

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1997

Acta Cytologica

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Concurrent Fluorescence <i>in Situ </i>Hybridization and Immunocytochemistry for the Detection of Chromosome Aberrations in Exfoliated Bronchial Epithelial Cells

Cited by 6 publications

References 5 publications

Saccomanno Smear Slides and Megafunnel Slides for Sputum Specimens

Saccomanno Smear Slides and Megafunnel Slides for Sputum Specimens

The Liverpool Lung Project: a molecular epidemiological study of early lung cancer detection

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