Concurrent changes in thermal tolerance thresholds and cellular heat stress response reveals novel molecular signatures and markers of high temperature acclimation in rainbow trout

Pandey, Anupam; Rajesh, Manchi; Baral, Pratibha; Sarma, Debajit; Tripathi, Priyanka H.; Akhtar, M. S.; Ciji, Alexander; Dubey, Maneesh Kumar; Pande, Veena; Sharma, Prakash; Kamalam, Biju Sam

doi:10.1016/j.jtherbio.2021.103124

Cited by 16 publications

(12 citation statements)

References 68 publications

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“…Unlike other molecular chaperones, HSP47 recognizes the folded state of its target, the collagen triple helix ( Widmer et al 2012 ). Expression of serpinh1b has been associated with thermal stress response in the rainbow trout ( Wang et al 2016 ; Pandey et al 2021 ). Although most of these genes do not show coding changes under selection, mutations over nearby noncoding sites might be driving the evolution of HSR regulation.…”

Section: Discussionmentioning

confidence: 99%

Genomics of Secondarily Temperate Adaptation in the Only Non-Antarctic Icefish

Rivera‐Colón

Rayamajhi

Minhas

et al. 2023

Molecular Biology and Evolution

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White-blooded Antarctic icefishes, a family within the adaptive radiation of Antarctic notothenioid fishes, are an example of extreme biological specialization to both the chronic cold of the Southern Ocean and life without hemoglobin. As a result, icefishes display derived physiology that limits them to the cold and highly oxygenated Antarctic waters. Against these constraints, remarkably one species, the pike icefish Champsocephalus esox, successfully colonized temperate South American waters. To study the genetic mechanisms underlying secondarily temperate adaptation in icefishes, we generated chromosome-level genome assemblies of both C. esox and its Antarctic sister species, Champsocephalus gunnari. The C. esox genome is similar in structure and organization to that of its Antarctic congener; however, we observe evidence of chromosomal rearrangements coinciding with regions of elevated genetic divergence in pike icefish populations. We also find several key biological pathways under selection, including genes related to mitochondria and vision, highlighting candidates behind temperate adaptation in C. esox. Substantial antifreeze glycoprotein (AFGP) pseudogenization has occurred in the pike icefish, likely due to relaxed selection following ancestral escape from Antarctica. The canonical AFGP locus organization is conserved in C. esox and C. gunnari, but both show a translocation of two AFGP copies to a separate locus, previously unobserved in cryonotothenioids. Altogether, the study of this secondarily temperate species provides an insight into the mechanisms underlying adaptation to ecologically disparate environments in this otherwise highly specialized group.

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Section: Discussionmentioning

confidence: 99%

Genomics of Secondarily Temperate Adaptation in the Only Non-Antarctic Icefish

Rivera‐Colón

Rayamajhi

Minhas

et al. 2023

Molecular Biology and Evolution

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“…2021; Pandey et al. 2021). Nevertheless, the gene expression and enzyme activity of CAT decreased with rising temperature.…”

Section: Discussionmentioning

confidence: 99%

Effect of temperature on growth, survival, and chronic stress responses of Arctic Grayling juveniles

Carrillo‐Longoria,

Gaylord,

Andrews

et al. 2023

Trans Am Fish Soc

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ObjectiveArctic Grayling Thymallus arcticus are Holarctically distributed, with a single native population in the conterminous United States occurring in the Big Hole River, Montana, where water temperatures can fluctuate throughout the year from 8°C to 18°C. A gradual increase in mean water temperature has been reported in this river over the past 20 years due to riparian habitat changes and climate change effects. We hypothesized that exposing Arctic Grayling to higher temperatures would result in lower survival, decreased growth, and increased stress responses.MethodsOver a 144‐day trial, Arctic Grayling juveniles were subjected to water temperatures ranging from 8°C to 26°C to measure the effects on growth, survival, gene expression, and antioxidant enzyme activity.ResultFish growth increased with increasing water temperature up to 18°C, beyond which survival was reduced. Fish did not survive at temperatures above 22°C. In response to temperatures above 16°C, 3.0‐fold and 1.5‐fold increases in gene expression were observed for superoxide dismutase (SOD) and glutathione peroxidase (GPx), respectively, but no changes were seen in the gene expression ratio of heat shock protein 70 to heat shock protein 90. Activities of the SOD and GPx enzymes also rose at temperatures above 16°C, indicating heightened oxidative stress. Catalase gene expression and enzyme activity decreased with rising temperatures, suggesting a preference for the GPx pathway, as GPx could also be providing help with lipid peroxidation. An increase in thiobarbituric acid reactive substances was also recorded, which corresponded with rising temperatures.ConclusionOur findings thus underscore the vulnerability of Arctic Grayling to minor changes in water temperature. Further increases in mean water temperature could significantly compromise the survival of Arctic Grayling in the Big Hole River.

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“…SerpinH1 (also known as Hsp47 ) is a chaperone involved in the biosynthesis of collagen at the ER [ 76 ], with a key role in the restoration of homeostasis during high-temperature stress and oxidative stress [ 22 ]. It was shown to be a good biomarker for thermal stress in several salmonid species, including rainbow trout ( Oncorhynchus mykiss ), sockeye salmon ( Oncorhynchus nerka ), and Chinook salmon ( Oncorhynchus tshawytscha ) [ 3 , 77 ], as well as zebrafish [ 78 ], evidencing that sherpinh1 is one of the most suitable transcriptional biomarkers of high-temperature stress in fish. This was also the case for red cusk-eel in this study, representing a useful tool to evaluate thermal stress status in this species under a climate change scenario.…”

Section: Discussionmentioning

confidence: 99%

High-Temperature Stress Effect on the Red Cusk-Eel (Geypterus chilensis) Liver: Transcriptional Modulation and Oxidative Stress Damage

Dettleff

Zuloaga

Fuentes

et al. 2022

Biology

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Environmental stressors, such as temperature, are relevant factors that could generate a negative effect on several tissues in fish. A key fish species for Chilean aquaculture diversification is the red cusk-eel (Genypterus chilensis), a native fish for which knowledge on environmental stressors effects is limited. This study evaluated the effects of high-temperature stress on the liver of red cusk-eel in control (14 °C) and high-temperature (19 °C) groups using multiple approaches: determination of plasmatic hepatic enzymes (ALT, AST, and AP), oxidative damage evaluation (AP sites, lipid peroxidation, and carbonylated proteins), and RNA-seq analysis. High-temperature stress generated a significant increase in hepatic enzyme activity in plasma. In the liver, a transcriptional regulation was observed, with 1239 down-regulated and 1339 up-regulated transcripts. Additionally, high-temperature stress generated oxidative stress in the liver, with oxidative damage and transcriptional modulation of the antioxidant response. Furthermore, an unfolded protein response was observed, with several pathways enriched, as well as a heat shock response, with several heat shock proteins up regulated, suggesting candidate biomarkers (i.e., serpinh1) for thermal stress evaluation in this species. The present study shows that high-temperature stress generated a major effect on the liver of red cusk-eel, knowledge to consider for the aquaculture and fisheries of this species.

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Concurrent changes in thermal tolerance thresholds and cellular heat stress response reveals novel molecular signatures and markers of high temperature acclimation in rainbow trout

Cited by 16 publications

References 68 publications

Genomics of Secondarily Temperate Adaptation in the Only Non-Antarctic Icefish

Genomics of Secondarily Temperate Adaptation in the Only Non-Antarctic Icefish

Effect of temperature on growth, survival, and chronic stress responses of Arctic Grayling juveniles

High-Temperature Stress Effect on the Red Cusk-Eel (Geypterus chilensis) Liver: Transcriptional Modulation and Oxidative Stress Damage

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