Concordant Mitochondrial and Nuclear DNA Partitions Define Evolutionarily Significant Units in the Imperiled Pinewoods Darter, Etheostoma mariae (Pisces: Percidae)

Krabbenhoft, Trevor J.; Rohde, Fred C.; Leibman, Adena; Quattro, Joseph M.

doi:10.1643/cg-07-200

Cited by 8 publications

(4 citation statements)

References 31 publications

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“…This is reflected in our combined analyses of all three genes for both the maximum parsimony and the Bayesian inference approaches. The lack of phylogenetic resolution is attributable to the small number of nucleotide base-pair changes found among the Canadian sequences, the different mutation rates between mitochondrial (1.4%-2.6%/million years) and nuclear (0.14%-0.26%/million years) DNA genes (Glor et al 2004;Godinho et al 2008;Krabbenhoft et al 2008;Timpe et al 2009), and the very different effective population sizes of mitochondrial versus nuclear markers. The observed genetic uniformity of Canadian lizards may be indicative of insufficient time since colonization for neutral mutations to have accumulated in the mitochondrial and nuclear DNA genes examined (Bos et al 2008;Godinho et al 2008;Moore et al 2008;Hansen et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Genetic structure of the endangered Greater Short-horned Lizard (Phrynosomahernandesi) in Canada: evidence from mitochondrial and nuclear genes

2014

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The northern edge of the range of the Greater Short-horned Lizard (Phrynosoma hernandesi Girard, 1858) occurs in western Canada, where the species has "endangered" status and exhibits a patchy distribution. Phylogenetic inference and genetic analyses were employed to investigate the genetic structure of P. hernandesi throughout its Canadian range. One nuclear and two mitochondrial DNA genes were sequenced from 94 lizard tail tips. Overall, sequences from lizards from both Alberta and Saskatchewan displayed very little variability, and the consistent clustering of all the P. hernandesi mitochondrial and nuclear DNA sequences from Canada in both phylogenetic and population genetic analyses is consistent with the lizards from all sampled localities having originated from a single glacial refugium, and with being, until recently (or currently) interconnected genetically. The genetic data obtained so far furnish no information useful for interpreting the species' present-day patchy distribution patterns or for formulating conservation strategies.

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Section: Discussionmentioning

confidence: 99%

Genetic structure of the endangered Greater Short-horned Lizard (Phrynosomahernandesi) in Canada: evidence from mitochondrial and nuclear genes

2014

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“…Several lineages of fishes have diversified within, as opposed to between, major river systems in the highlands of eastern North America (Page et al. 1992; Ceas & Page 1997; Hardman 2004; Keck & Near 2008; Krabbenhoft et al. 2008; Hollingsworth & Near 2009).…”

Section: Introductionmentioning

confidence: 99%

A young clade repeating an old pattern: diversity inNothonotusdarters (Teleostei: Percidae) endemic to the Cumberland River

Keck

Near

2010

Molecular Ecology

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Hypotheses of diversification in eastern North American freshwater fishes have focused primarily on allopatric distributions of species between disjunct highland areas and major river systems. However, these hypotheses do not fully explain the rich diversity of species within highland regions and river systems. Relatively old diversification events at small geographic scales have been observed in the Barcheek Darter subclade that occurs in the Cumberland River drainage (CRD) in Kentucky and Tennessee, United States of America, but it is unknown if this pattern is consistent in other darter subclades. We explored phylogeographic diversity in two species of Nothonotus darters, N. microlepidus and N. sanguifluus, endemic to the CRD to compare phylogenetic patterns between Barcheek Darters and species of Nothonotus. We collected sequence data for a mitochondrial gene (cytb) and three nuclear genes (MLL, S7 and RAG1) from 19 N. microlepidus and 35 N. sanguifluus specimens. Gene trees were estimated using maximum likelihood and Bayesian methods, and a 'species tree' was inferred using a Bayesian method. These trees indicate that species diversity in Nothonotus is underestimated. Five distinct lineages were evident, despite retained ancestral polymorphism and unsampled extirpated populations. Comparison of chronograms for Barcheek Darters and Nothonotus revealed that microendemism resulting from species diversification at small geographic scales in the CRD is a consistent pattern in both old and young darter subclades. Our analyses reveal that geographic isolating mechanisms that result in similar phylogeographic patterns in the CRD are persistent through long expanses of evolutionary time.

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“…within drainage) genetic structure for the collis group. Interestingly, intradrainage genetic structure was recently reported for the pinewoods darter Etheostoma mariae, a codistributed congener of the collis group (Krabbenhoft, Rohde & Quattro, 2008). If present, such fine-scale genetic structure could result in recognition of multiple collis group ESUs per drainage.…”

Section: Discussionmentioning

confidence: 94%

Evolutionarily independent genes and genomes and insights on genetic structure, evolution and conservation of the collis group of darters

Oswald

Rohde

Arndt

et al. 2009

Animal Conservation

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Comparing variation across evolutionarily independent characters, notably nuclear and mitochondrial genes, yields a more robust estimate of diversification than is generally recovered from individual characters. Patterns of variation across multiple molecular markers from the mitochondrial (16SrRNA, cytochrome b) and nuclear (ldhA6 and aldB) genomes were examined from six populations of Etheostoma collis and two populations of Etheostoma saludae, species aligned in the collis groups. Phylogenetic analyses revealed that sequence variation among individuals from the Roanoke, Tar and Neuse Rivers and the Catawba and Pee Dee Rivers, respectively, form highly supported, deeply divergent clades. Relationships of alleles sampled from Saluda River E. saludae and Cape Fear River E. collis to these lineages are unresolved, but all groups are reciprocally monophyletic for both nuclear and mtDNA loci. Phylogenetic analyses suggest that historical factors have had a strong influence on the distribution of genetic variation among populations. Genetic variation within the collis group is consistent with all previously proposed taxonomic hypotheses for the collis group, providing no taxonomic insights. From a conservation standpoint, each population of the collis group is an ESU, thereby warranting a drainage-specific management strategy.

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Concordant Mitochondrial and Nuclear DNA Partitions Define Evolutionarily Significant Units in the Imperiled Pinewoods Darter, Etheostoma mariae (Pisces: Percidae)

Cited by 8 publications

References 31 publications

Genetic structure of the endangered Greater Short-horned Lizard (Phrynosomahernandesi) in Canada: evidence from mitochondrial and nuclear genes

Genetic structure of the endangered Greater Short-horned Lizard (Phrynosomahernandesi) in Canada: evidence from mitochondrial and nuclear genes

A young clade repeating an old pattern: diversity inNothonotusdarters (Teleostei: Percidae) endemic to the Cumberland River

Evolutionarily independent genes and genomes and insights on genetic structure, evolution and conservation of the collis group of darters

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