Concordance Study Between the AmpFℓSTR^® MiniFiler^TM PCR Amplification Kit and Conventional STR Typing Kits*

Abstract

Table 1 and encompass the loci D13S317 (n = 14) and D16S539 (n = 10) as well as D18S51 (n = 1), D7S820 (n = 1), and CSF1PO (n = 1). Genotyping discrepancies between the Identifiler and MiniFiler kits were confirmed by reamplification of the samples and further testing using the PowerPlex Ò 16 kit in many cases. DNA sequence analysis was also performed in order to understand the nature of the genetic variations causing the allele dropout or apparent repeat unit shift.

Concordance study between the ParaDNA ® Intelligence Test, a Rapid DNA profiling assay, and a conventional STR typing kit (AmpFlSTR ® SGM Plus ® )

“…Identifiler and AmpFlSTR Minifiler [10], 99.77% between AmpFlSTR Identifiler and a custom Mini STR assay [11] and 99.97% between PowerPlex ESI 16 and AmpFlSTR SGM Plus [13]. The ParaDNA Intelligence Assay can therefore be considered to have high concordance of allele calling with a UK standard STR typing kit.…”

“…Concordance studies are typically conducted on a number (typically several hundred or more) of samples from individuals in a reference population or population subgroup using one profiling kit for which profiling data has been obtained using another product, thus establishing concordance between the profiles generated by the two products [4,10,11]. In addition, population studies can provide data on allele frequencies anticipated in the reference population or populations [5,6,12].…”

Internal validation of the AmplFlSTR MiniFiler kit

“…At 15:1 most of the minor female alleles could not be www.elsevier.com/locate/FSIGSS Available online at www.sciencedirect.com Known samples and simulated case samples from the German DNA Profiling group-Stain Commission (GED-NAP) were tested using the MiniFiler kit. DNA profiles obtained from the known and questioned items (GEDNAP 28, 29, 32 and 33) were compared with those already obtained for the SGM Plus kit [1]. The typing results were consistent between the two kits and with those of the other participant laboratories.…”

Extração de DNA para análise da amelogenina em amostras fixadas em formalina, incluídas em parafina e armazenadas por 1 e 5 anos no Departamento de Patologia da Universidade Federal de São Paulo

“…O kit de STR Identifi ler® é rotineiramente utilizado para estudos de genética forense e, mais recentemente, os mini-STR têm sido adotados para análise de perfi s genéticos para identifi cação humana 14 . Nestes kits, o primer da amelogenina (106-112pb) está incluído em uma reação única e multiplex de PCR.…”