Concordance between the In Vivo Content of Neurospecific Proteins (BDNF, NSE, VILIP-1, S100B) in the Hippocampus and Blood in Patients with Epilepsy

Tikhonova, Maria A.; Shvaikovskaya, Anna A.; Zhanaeva, Svetlana Y.; Moysak, Galina I.; Akopyan, Anna A.; Rzaev, Jamil A.; Danilenko, Konstantin V.; Aftanas, Lyubomir I.

doi:10.3390/ijms25010502

Cited by 1 publication

(1 citation statement)

References 49 publications

(57 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…IHC analysis for a marker of proliferating cells Ki67 was performed in 30 μm thick frozen slices of the tumor tissue as described in detail earlier [ 55 ]. Primary antibodies against Ki67 (1:200; ab16667, Abcam, Cambridge, UK) and secondary goat-anti-rabbit Alexa Flur 488 antibodies (1:600; ab150077, Abcam, Cambridge, UK) were used to evaluate the Ki67 expression.…”

Section: Methodsmentioning

confidence: 99%

Antitumor Effects of an Anthocyanin-Rich Grain Diet in a Mouse Model of Lewis Lung Carcinoma

Tikhonova,

Shoeva,

Tenditnik

et al. 2024

IJMS

Self Cite

View full text Add to dashboard Cite

Functional foods enriched with plant polyphenol anthocyanins attract particular attention due to their health-promoting properties, including antitumor activity. We evaluated the effects of a grain diet rich in anthocyanins in a mouse model of Lewis lung carcinoma. Mice of the C57BL/6 strain were fed with wheat of near-isogenic lines differing in the anthocyanin content for four months prior to tumor transplantation. Although a significant decrease in the size of the tumor and the number of metastases in the lungs was revealed in the groups with both types of grain diet, the highest percentage of animals without metastases and with attenuated cell proliferation in the primary tumor were observed in the mice with the anthocyanin-rich diet. Both grain diets reduced the body weight gain and spleen weight index. The antitumor effects of the grain diets were associated with the activation of different mechanisms: immune response of the allergic type with augmented interleukin(IL)-9 and eotaxin serum levels in mice fed with control grain vs. inhibition of the IL-6/LIF system accompanied by a decrease in the tumor-associated M2 macrophage marker arginase 1 gene mRNA levels and enhanced autophagy in the tumor evaluated by the mRNA levels of Beclin 1 gene. Thus, anthocyanin-rich wheat is suggested as a promising source of functional nutrition with confirmed in vivo antitumor activity.

show abstract

Section: Methodsmentioning

confidence: 99%