Conclusion

Comparative studies on trifluoroethanol (TFE) state of a thermophilic α-amylase and its mesophilic counterpart: limited proteolysis, conformational analysis, aggregation and reactivation of the enzymes

“…A correlation between sites of proteolysis and segmental mobility in thermostable proteins has also been previously established [30,31]. Our comparative studies indicate that the BLA has shown a greater resistance to digestion by the trypsin, thermolysin, and proteinase K [31,and Khajeh,unpublished data].…”

“…Previous studies have shown that the specific or preferential fission of peptide bond(s) by limited proteolysis [30,31] is a useful way to probe structural properties of globular proteins [32,33]. Limited proteolysis has been performed in the presence of different amounts of TFE by incubating BLA and BAA with the thermolysin under various experimental conditions of enzyme to substrate ratio, temperature and duration of incubation.…”