Concise Synthesis of Glycerophospholipids

Mukhopadhyay, Tufan K.; Trauner, Dirk

doi:10.1021/acs.joc.2c02096

Cited by 13 publications

(5 citation statements)

References 34 publications

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“…In animals, phospholipids are hydrolyzed into glycerol, phosphate, choline, and ethanolamine by a series of phospholipases. Glycerophospholipids are major lipids in cell membranes and play important roles in cell signaling, G protein-coupled receptors, and ion transport ( 71 ). In the present study, serum levels of glycerophosphocholine, 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine, and 1-palmitoyl-sn-glycero-3-phosphocholine were decreased in Dazu black goats in response to HS ( Figure 8 ).…”

Section: Discussionmentioning

confidence: 99%

Effect of heat stress on blood biochemistry and energy metabolite of the Dazu black goats

Wang,

Zhang,

et al. 2024

Front. Vet. Sci.

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The objective of this study was to determine the effects of heat stress (HS) on physiological, blood biochemical, and energy metabolism in Dazu black goats. Six wether adult Dazu black goats were subjected to 3 experimental periods: high HS (group H, temperature-humidity index [THI] > 88) for 15 d, moderate HS (group M, THI was 79-88) for 15 d, and no HS (group L, THI < 72) for 15 d. Rectal temperature (RT) and respiratory rate (RR) were determined on d 7 and 15 of each period, and blood samples were collected on d 15 of each period. All goats received glucose (GLU) tolerance test (GTT) and insulin (INS) tolerance test on d 7 and d 10 of each period. The results showed that HS decreased dry matter intake (DMI) and INS concentrations (p < 0.05), and increased RT, RR, non-esterified fatty acid (NEFA), cortisol (COR), and total protein (TP) concentrations (p < 0.05). Compared to group L, the urea nitrogen (BUN) concentration increased and GLU concentration decreased in group H (p < 0.05).During the GTT, the area under the curve (AUC) of GLU concentrations increased by 12.26% (p > 0.05) and 40.78% (p < 0.05), and AUC of INS concentrations decreased by 26.04% and 14.41% (p < 0.05) in groups H and M compared to group L, respectively. The INS concentrations were not significant among the three groups (p > 0.05) during the ITT. A total of 60 differentially expressed metabolites were identified in response to groups H and M. In HS, changes in metabolites related to carbohydrate metabolism and glycolysis were identified (p < 0.05). The metabolites related to fatty acid βoxidation accumulated, glycogenic and ketogenic amino acids were significantly increased, while glycerophospholipid metabolites were decreased in HS (p < 0.05). HS significantly increased 1-methylhistidine, creatinine, betaine, taurine, taurolithocholic acid, inosine, and hypoxanthine, while decreasing vitamin E in blood metabolites (p < 0.05). In summary, HS changed the metabolism of fat, protein, and energy, impaired GLU tolerance, and mainly increased amino acid metabolism to provide energy in Dazu black goats.

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Section: Discussionmentioning

confidence: 99%

Effect of heat stress on blood biochemistry and energy metabolite of the Dazu black goats

Wang,

Zhang,

et al. 2024

Front. Vet. Sci.

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“…These two pathways were driven by deoxyguanosine and ethanolamine, respectively. The relative concentration of ethanolamine was lower in STC, compared to SUF sheep, and its associated glycerophospholipid metabolism was found to be a strong indicator of copper toxicity in pig kidney (Qiao et al, 2021;Bi et al, 2022;Mukhopadhyay and Trauner, 2023). Reduced relative concentration of ethanolamine in STC may suggest non-toxic liver tissue.…”

Section: Pathway Name Fdr Associated Metabolitesmentioning

confidence: 93%

Lipopolysaccharide-induced alterations in the liver metabolome of St. Croix and Suffolk sheep

Johnson,

Bentley,

Bowdridge

et al. 2024

Front. Anim. Sci.

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The development of resistance in parasites due to overuse of anthelmintics has resulted in a marked decrease in the efficacy of these drug classes. Recent research efforts have focused on exploring alternatives such as selection for parasite-resistant breeds with the implication that immunocompetence may align with parasite resistance. Two breeds that are often investigated are the St. Croix (STC), a resistant hair breed, and Suffolk (SUF), a susceptible wool breed sheep. The liver plays a vital role in metabolism in the body and metabolizes lipopolysaccharide (LPS), which triggers whole body response through the production of appropriate metabolites, cytokines and immune cells. The objective of this study was to investigate the breed differences in liver metabolome of sheep, with divergent resistance to parasites, in response to LPS. Both STC and SUF sheep (n = 9/breed) were challenged with LPS intravenously. Rectal temperatures and sheep grimace score (SGS) were recorded hourly, for each animal, and averaged across the study for both breeds. The average rectal temperature throughout the study was similar for STC and SUF sheep (40.4°C and 40.2°C respectively), but the pattern of response was different. STC had an average SGS of 0.8 while SUF had an average of 3.3. Liver biopsies were collected from 3 sheep that were not challenged with LPS (HR0; n = 3/breed), two hours post-challenge (HR2; n = 3/breed), and six hours post-challenge (HR6; n = 3/breed). Liver tissue samples were subjected to quantitative untargeted metabolome analysis using chemical isotope labeling/liquid chromatography-mass spectrometry. Pathway analysis of the HR0 metabolome data revealed that 8 pathways (and their associated metabolites) including beta-alanine metabolism, arginine and proline metabolism and glutathione metabolism were altered (false discovery rate-adjusted P-value (FDR) ≤ 0.05) between STC and SUF sheep. At HR2, 10 altered pathways such as folate biosynthesis, taurine and hypotaurine metabolism, and glutathione metabolism. At HR6, only 2 pathways (glycerophospholipid metabolism and purine metabolism) were altered (FDR ≤ 0.05) between STC and SUF sheep. Results highlight the differences in hepatic metabolome and physiological response to LPS challenge that exist between SUF and STC. These findings suggest breed-specific differences in metabolic response to immune challenge, potentially influencing the divergent resistance of the two breeds to parasitic infections.

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“…Bio-membranes contain a significant amount of glycerophospholipids, which are known to control several cellular signaling events involving ion channels or G-protein coupled receptors. Protein translocation, apoptosis, inflammation, and neurogenesis are crucial in calcium homeostasis [80]. These studies suggested that active metabolic pathways and high abundance of metabolite contents may be important mechanisms for the resistance to PBD by the HR cultivars.…”

Section: Metabolomic Profiling Of the Stems Of Hr And Hs Sugarcane Cu...mentioning

confidence: 95%

Analysis of metabolome and microbiome revealed the resistance mechanisms in sugarcane cultivars with high resistance to pokkah boeng disease

Xiao,

Chen,

Liang

et al. 2024

Chem. Biol. Technol. Agric.

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Background Endophytes are reported to play a role in resistance against plant pathogens. Understanding the metabolite-mediated endophytic microbiota composition in plants provides insights to improve plant stress resistance. In this study, via metabolome and microbiome analyses, we aimed to elucidate the resistance mechanism of sugarcane cultivars with high resistance to sugarcane pokkah boeng disease (PBD). The endophytic microbial composition and metabolites in the stems of various sugarcane cultivars with high resistance (HR) or high susceptibility (HS) to PBD were analyzed. Results The results revealed that the endophytic fungi with biocontrol effects such as Shinella, Dechloromonas, and Microbacter were significantly enriched, and the abundance of pathogenic fungi such as Fusarium, Ramichloridium, Scleroramularia, Phaeosphaeriopsis, Sarocladium, Zygophiala, Gibberella, Pseudocercospora, Cyphellophora, Monocillium, Apiotrichum, Microsphaeropsis, and Scleroramularia significantly reduced in the stems of HR cultivars. Additionally, six metabolites [citric acid, isocitrate, malic acid, PC(16:0/0:0), phosphocholine, and lysoPC(16:0)] were significantly related to the endophytes in the stems of HR cultivars. Conclusions These results suggested that more abundance of antagonistic microbes and highly active metabolic functions of endophytes in the HR cultivars were the important mechanisms underlying their higher resistance to PBD. Graphical abstract

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Concise Synthesis of Glycerophospholipids

Cited by 13 publications

References 34 publications

Effect of heat stress on blood biochemistry and energy metabolite of the Dazu black goats

Effect of heat stress on blood biochemistry and energy metabolite of the Dazu black goats

Lipopolysaccharide-induced alterations in the liver metabolome of St. Croix and Suffolk sheep

Analysis of metabolome and microbiome revealed the resistance mechanisms in sugarcane cultivars with high resistance to pokkah boeng disease

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