Concise Review: Tissue Engineering of Urinary Bladder; We Still Have a Long Way to Go?

Adamowicz, Jan; Pokrywczyńska, Marta; Breda, Shane Vontelin van; Kloskowski, Tomasz

doi:10.1002/sctm.17-0101

Cited by 56 publications

(68 citation statements)

References 105 publications

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“…The translation of increasing amounts of basic research data to clinical practice remains problematic. Researchers have identified vascularization, innervation, and fibrosis issues within the bladder related to an unfavorable environment . Graft‐related vascular issues are the main reason for delays in the development of viable bladder regeneration projects.…”

Section: Bladder Disorders: Several Decades and Animal Models Latermentioning

confidence: 99%

“…Because spontaneous angiogenesis after with proangiogenic factors such as vascular endothelial growth factor (VEGF), matrix metalloproteases, basic fibroblast growth biomaterial implantation may not be fast enough, thereby causing loss of neobladder function, graft prevascularization has been proposed. Stimulating vascularization by enriching implants factor, platelet‐derived growth factor, angiotensin, and hepatocyte growth factor has been tried with limited success . The de novo design and generation of vascular networks using customizable three dimensional (3D) bioprinting technology shows promise for the future …”

Section: Bladder Disorders: Several Decades and Animal Models Latermentioning

confidence: 99%

“…The use of selective and nonselective pharmacological agents with antifibrotic activity (pirfenidone, steroids) has limited success. As most efforts to‐date target selective fibrotic pathways, commonly involving transforming growth factor‐β (TGF‐β), researchers now propose to investigate coordinated suppression of more than one profibrotic cascade . Innervation issues include re‐establishing neuronal transmission and autonomic neobladder activity via the development of neuronal network substitutes.…”

Section: Bladder Disorders: Several Decades and Animal Models Latermentioning

confidence: 99%

“…SCs differentiating into neurons could be an alternative . In this context, researchers have proposed to identify appropriate sites within the central nervous system with preserved neurogenesis from where biopsies could provide neuronal precursor cells without causing damage …”

Section: Bladder Disorders: Several Decades and Animal Models Latermentioning

confidence: 99%

“…Regenerative bladder replacement has involved the use of scaffolds that are either acellular or seeded with SCs. These scaffolds are degradable, biocompatible materials that promote tissue development . Research into the use of SCs for female SUI is close to reaching clinical maturity with autologous muscle–derived cells (AMDCs) used to augment urethral sphincter function in women with an intrinsic sphincteric deficiency (ISD).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Stem cells and lower urinary tract dysfunction: Has its potential finally reached clinical maturity? ICI‐RS2018

Chermansky

Mitsogiannis

Abrams

et al. 2019

Neurourology and Urodynamics

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Aims: Efforts to engineer and repair genitourinary tissue to treat lower urinary tract dysfunction (LUTD) have recently increased thanks in part to advances in stem cell (SC) research. At the International Consultation on Incontinence-Research Society meeting in Bristol in 2018 a proposal was convened to address the question: has the potential of SCs in treating LUTD reached clinical maturity? Methods: The proposal conducted a literature review and an expert consensus meeting focusing on available data from animal models and clinical trials involving the use of SCs for LUTD. Results: To date, there are only small studies investigating bladder replacement using scaffolds with or without SC. Results have been conflicting because of the variability in cell numbers, biomaterials types, and graft surface differences. Similarly, preclinical results suggest a possible role of SC in bladder outlet obstruction (BOO); however, SC clinical trials for BOO are lacking. Research into the use of SC for female stress urinary incontinence (SUI) is close to reaching clinical maturity. In the Canadian phase 3 randomized controlled trial (RCT), a beneficial effect of adult muscle-derived cells (AMDC) over placebo was detected in reducing the frequency of incontinence episodes, especially after prior anti-incontinence surgery. Only two small studies have been published on male SUI.Conclusions: Questions remain regarding the mechanism of action of SC injected into the LUT and the viability of cells seeded onto grafts placed into the LUT. Also, the optimal time for intervention with SC therapy in the LUT remains to be elucidated. K E Y W O R D Sbladder, bladder outlet obstruction, incontinence, neurogenic, regenerative, stem cells

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Section: Bladder Disorders: Several Decades and Animal Models Latermentioning

confidence: 99%

Section: Bladder Disorders: Several Decades and Animal Models Latermentioning

confidence: 99%

Section: Bladder Disorders: Several Decades and Animal Models Latermentioning

confidence: 99%

Section: Bladder Disorders: Several Decades and Animal Models Latermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Stem cells and lower urinary tract dysfunction: Has its potential finally reached clinical maturity? ICI‐RS2018

Chermansky

Mitsogiannis

Abrams

et al. 2019

Neurourology and Urodynamics

View full text Add to dashboard Cite

show abstract

Engineering pedicled vascularized bladder tissue for functional bladder defect repair

Chen

Wang

et al. 2022

Bioengineering & Transla Med

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An engineered bladder construct that mimics the structural and functional characteristics of native bladder is a promising therapeutic option for bladder substitution. We previously showed that pedicled vascularized smooth muscle tissue fabricated by grafting smooth muscle cell (SMC) sheets onto an axial capsule vascular bed had the potential for reliable bladder reconstruction. In this study, we investigated the feasibility of buccal mucosa graft (BMG) integration with the pedicled vascularized smooth muscle tissue to generate a full‐layer pedicled vascularized bladder construct. BMG transplanted onto vascularized smooth muscle tissue showed good survival and developed into a pedicled vascularized bladder construct with full‐layer structures, appropriate thickness, abundant vascularization, and effective barrier function. Then the full‐thickness bladder defects were, respectively, reconstructed by pedicled capsule tissue (pedicled capsule group), nonpedicled vascularized bladder construct (nonpedicled construct group), and pedicled vascularized bladder construct (pedicled construct group). The picrosirius red (PSR) staining and immunohistochemistry results showed minimal fibrosis, maximal smooth muscle proportion, and high vascular density in the pedicled construct group. A continuous mucosal layer was observed only in the pedicled construct group. Moreover, morphological and functional studies revealed better bladder compliance and good ductility in the pedicled construct group. Overall, these results suggested that the BMG could be well integrated with vascularized smooth muscle tissue and generated a pedicled, fully vascularized, and structurally organized bladder construct, which facilitated structural remodeling and functional recovery and could become an alternative to bladder reconstruction.

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Biodegradable polyurethane scaffolds in regenerative medicine: Clinical translation review

Pedersen

Kim

Wagner

2022

J Biomedical Materials Res

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Early explorations of tissue engineering and regenerative medicine concepts commonly utilized simple polyesters such as polyglycolide, polylactide, and their copolymers as scaffolds. These biomaterials were deemed clinically acceptable, readily accessible, and provided processability and a generally known biological response. With experience and refinement of approaches, greater control of material properties and integrated bioactivity has received emphasis and a broadened palette of synthetic biomaterials has been employed. Biodegradable polyurethanes (PUs) have emerged as an attractive option for synthetic scaffolds in a variety of tissue applications because of their flexibility in molecular design and ability to fulfill mechanical property objectives, particularly in soft tissue applications. Biodegradable PUs are highly customizable based on their composition and processability to impart tailored mechanical and degradation behavior. Additionally, bioactive agents can be readily incorporated into these scaffolds to drive a desired biological response. Enthusiasm for biodegradable PU scaffolds has soared in recent years, leading to rapid growth in the literature documenting novel PU chemistries, scaffold designs, mechanical properties, and aspects of biocompatibility. Despite the enthusiasm in the field, there are still few examples of biodegradable PU scaffolds that have achieved regulatory approval and routine clinical use. However, there is a growing literature where biodegradable PU scaffolds are being specifically developed for a wide range of pathologies and where relevant pre‐clinical models are being employed. The purpose of this review is first to highlight examples of clinically used biodegradable PU scaffolds, and then to summarize the growing body of reports on pre‐clinical applications of biodegradable PU scaffolds.

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Concise Review: Tissue Engineering of Urinary Bladder; We Still Have a Long Way to Go?

Cited by 56 publications

References 105 publications

Stem cells and lower urinary tract dysfunction: Has its potential finally reached clinical maturity? ICI‐RS2018

Stem cells and lower urinary tract dysfunction: Has its potential finally reached clinical maturity? ICI‐RS2018

Engineering pedicled vascularized bladder tissue for functional bladder defect repair

Biodegradable polyurethane scaffolds in regenerative medicine: Clinical translation review

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