Concise Review: Bioprinting of Stem Cells for Transplantable Tissue Fabrication

Leberfinger, Ashley N.; Ravnic, Dino J.; Dhawan, Aman; Özbolat, İbrahim T.

doi:10.1002/sctm.17-0148

Cited by 143 publications

(98 citation statements)

References 82 publications

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“…Perhaps due to the urgent nature of this clinical condition, the application of 3D bioprinting in the cardiovascular domain has seen rapid progress in recent years. Several animal studies have already shown that 3D bioprinted cardiac patches have the ability to reduce fibrosis, hypertrophy, and infarct extent [70][71][72][73][74][75]. The impact will extend beyond transplantation to include disease modeling and drug studies, as we have seen for other tissues such as skin.…”

Section: Cardiovascular Tissuementioning

confidence: 95%

Clinical Perspectives on 3D Bioprinting Paradigms for Regenerative Medicine

Loai¹,

Kingston²,

Wang³

et al. 2019

Regen Med Front.

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Three-dimensional (3D) bioprinting is an emerging manufacturing technology that layers living cells and biocompatible natural or synthetic materials to build complex, functional living tissue with the requisite 3D geometries. This technology holds tremendous promise across a plethora of applications as diverse as regenerative medicine, pathophysiological studies, and drug testing. Despite some success demonstrated in early attempts to recreate complex tissue structures, however, the field of bioprinting is very much in its infancy. There are a variety of challenges to building viable, functional, and lasting 3D structures, not the least of which is translation from a research to a clinical setting. In this review, the current translational status of 3D bioprinting is assessed for several major tissue types in the body (skin, bone/cartilage, cardiovascular, central/peripheral nervous systems, skeletal muscle, kidney, and liver), recent breakthroughs and current challenges are highlighted, and future prospects for this exciting research field are discussed. We begin with an overview of the technology itself, followed by a detailed discussion of the current approaches relevant for bioprinting different tissues for regenerative medicine.

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Section: Cardiovascular Tissuementioning

confidence: 95%

Clinical Perspectives on 3D Bioprinting Paradigms for Regenerative Medicine

Loai¹,

Kingston²,

Wang³

et al. 2019

Regen Med Front.

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“…However, many challenges must be overcome before bioprinting technology is used routinely in a clinical setting (Leberfinger et al, 2017).…”

Section: Advanced Scaffold Production Techniques Electrospinning Of Nmentioning

confidence: 99%

Building an Artificial Stem Cell Niche: Prerequisites for Future 3D‐Formation of Inner Ear Structures—Toward 3D Inner Ear Biotechnology

Groot

Sliedregt

Benthem

et al. 2019

The Anatomical Record

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In recent years, there has been an increased interest in stem cells for the purpose of regenerative medicine to deliver a wide range of therapies to treat many diseases. However, two‐dimensional cultures of stem cells are of limited use when studying the mechanism of pathogenesis of diseases and the feasibility of a treatment. Therefore, research is focusing on the strengths of stem cells in the three‐dimensional (3D) structures mimicking organs, that is, organoids, or organ‐on‐chip, for modeling human biology and disease. As 3D technology advances, it is necessary to know which signals stem cells need to multiply and differentiate into complex structures. This holds especially true for the complex 3D structure of the inner ear. Recent work suggests that although other factors play a role, the extracellular matrix (ECM), including its topography, is crucial to mimic a stem cell niche in vitro and to drive stem cells toward the formation of the tissue of interest. Technological developments have led to the investigation of biomaterials that closely resemble the native ECM. In the fast forward moving research of organoids and organs‐on‐chip, the inner ear has hardly received attention. This review aims to provide an overview, by describing the general context in which cells, matrix and morphogens cooperate in order to build a tissue, to facilitate research in 3D inner ear technology. Anat Rec, 303:408–426, 2020. © 2019 The Authors. The Anatomical Record published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists.

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“…Many exciting advances in the use of bioactive components for precision medicine have already been implemented in clinical settings for non‐3D printing applications, such as personalized gene therapies or matrix‐induced autologous chondrocyte implantation . Further, advances in gene therapy, cell culture, and cell manufacturing offer an array of exciting new components to incorporate into bioinks for precision medicine . While not the focus of this progress report, design of these inks, which can be broken into further discrete unit operators, has been detailed in previous reviews …”

Section: Introductionmentioning

confidence: 99%

“…While ease of analysis and access to patient data has improved for clinicians, many limitations still exist for the translation of precision medicine to patient treatment, including prohibitive costs, poor or nonexistent manufacturing www.advmat.de www.advancedsciencenews.com in gene therapy, cell culture, and cell manufacturing offer an array of exciting new components to incorporate into bioinks for precision medicine. [16,[23][24][25][26][27] While not the focus of this progress report, design of these inks, which can be broken into further discrete unit operators, has been detailed in previous reviews. [2,3,5,28] The next step in the process uses these inks to fabricate the constructs designed in the first step ( Figure 1C).…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Enabling Technologies in 3D Printing for Precision Medicine

2019

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Advances in areas such as data analytics, genomics, and imaging have revealed individual patient complexities and exposed the inherent limitations of generic therapies for patient treatment. These observations have also fueled the development of precision medicine approaches, where therapies are tailored for the individual rather than the broad patient population. 3D printing is a field that intersects with precision medicine through the design of precision implants with patient‐directed shapes, structures, and materials or for the development of patient‐specific in vitro models that can be used for screening precision therapeutics. Toward their success, advances in 3D printing and biofabrication technologies are needed with enhanced resolution, complexity, reproducibility, and speed and that encompass a broad range of cells and materials. The overall goal of this progress report is to highlight recent advances in 3D printing technologies that are helping to enable advances important in precision medicine.

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Concise Review: Bioprinting of Stem Cells for Transplantable Tissue Fabrication

Cited by 143 publications

References 82 publications

Clinical Perspectives on 3D Bioprinting Paradigms for Regenerative Medicine

Clinical Perspectives on 3D Bioprinting Paradigms for Regenerative Medicine

Building an Artificial Stem Cell Niche: Prerequisites for Future 3D‐Formation of Inner Ear Structures—Toward 3D Inner Ear Biotechnology

Recent Advances in Enabling Technologies in 3D Printing for Precision Medicine

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